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Catalogue No.


Cell Line Name


Cell Line Description

Aag2-AF5 is a single cell clone derived from the Aag2 cell line. Aag2-AF5 is immunocompetent for RNAi (siRNA and piRNA pathways) and NF-kB signalling.

General Info


Mosquito (Aedes aegypti - Yellow Fever Mosquito)

Unique to ECACC




These cells have a proven virus susceptibility for Dengue virus, Zika virus, Sindbis virus, Semliki Forest virus and Vesicular Stomatitis virus.

Tissue of Origin



Cell clusters and floating rounded cells


Propagation of viruses. Elucidation of molecular mechanisms of cellular response to viral infection.


None Stated

Culture Conditions

Cell Type


Subculture Routine

Subculture sub-confluent cultures (70-80%) as required (1-2 times a week) seeding cells between 2-5 x 10⁴ cells/cm². Remove culture medium and replace with fresh medium. Scrape monolayer of cells using cell scraper into the fresh media. Resuspend cells well by pipetting. Replace growth medium twice per week. Note: Cells may be slow to recover upon resuscitation and may have reduced viability. Cells may take up to 10 days to fully recover before subculturing. Cryopreservation medium: Complete growth medium containing 20% Foetal Bovine Serum and 10% (v/v) Dimethyl Sulphoxide (DMSO).

Culture Medium

L15 medium supplemented with 1% Non-Essential amino acids (NEAA), 1mM L-Glutamine, 10% Tryptose phosphate broth (TPB) and 10% Foetal Bovine Serum (FBS). Temperature: 28°C. Gassing: None.

Growth Mode


Additional Info


Kevin Maringer, University of Surrey, UK

Country of Origin


Hazard Group (ACDP)

Hazard Group (ACDP) 2



Varjak M, Maringer K, Watson M, Sreenu VB, Fredericks AC, Pondeville E, Donald CL, Sterk J, Kean J, Vazeille M, Failloux AB, Kohl A, Schnettler E. Aedes aegypti Piwi4 Is a Noncanonical PIWI Protein Involved in Antiviral Responses. mSphere. 2017 May 3;2(3). pii: e00144-17. doi: 10.1128/mSphere.00144-17. eCollection 2017 May-Jun.


Peleg J. Growth of arboviruses in primary tissue culture of Aedes aegypti embryos. Am. J. Trop. Med. Hyg. 17:219-223(1968). PubMed=4869110; DOI=10.4269/ajtmh.1968.17.219 Lan Q., Fallon A.M. Small heat shock proteins distinguish between two mosquito species and confirm identity of their cell lines.Am. J. Trop. Med. Hyg. 43:669-676(1990). PubMed=2267971; DOI=10.4269/ajtmh.1990.43.669 Barletta A.B.F., Silva M.C.L.N., Sorgine M.H.F. Validation of Aedes aegypti Aag- 2 cells as a model for insect immune studies. Parasites Vectors 5: 148 (2012). PubMed=22827926; DOI=10.1186/1756-3305-5-148

Available Formats

  • Frozen

If use of this culture results in a scientific publication, it should be cited in the publication as: Aag2-AF5 (ECACC 19022601).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.