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UK Health Security Agency

Rev-Erb alpha (NR1D1)

Rev-Erb alpha (NR1D1)

Catalogue No.

19061801

Cell Line Name

Rev-Erb alpha (NR1D1)

Cell Line Description

Murine hybridoma generated against human Rev-Erb alpha ligand binding domain (281-614). [NR1D1 is an alias for the Rev-Erb alpha protein. It stands for Nuclear Receptor family, group D, member 1]. The antibody subclass is IgG1.

Characteristics

Myeloma

P3x63/Ag 8.653/GFP/Bcl-2.11

Immunological Donor

His-tagged Rev-Erb alpha ligand-binding domain (aa281-614) protein.

Antibody Isotype

IgG1

Morphology

Spherical

Tissue of Origin

Hybrid

Culture Conditions

Cell Type

Hybridoma

Subculture Routine

Resuscitation: Remove cryopreservation medium by dilution in fresh growth medium and pellet cells at 250g and re-suspend cells at 2.5 x10⁵ cells/ml.Subculture the cells between 1-3 x 10⁵ cells/ml. Keep the cell density to less than 1 x 10⁶ cells/ml for optimal growth. Change medium every 2-3 days. Hybridoma growth should double every 24 hours. Loosely attached cells can be pipetted gently from off the plastic.Cryopreserve in either Bambanker (Fisher Scientific, NC9582225), or 90% Foetal Bovine Serum (FBS) + 10% Dimethyl Sulfoxide (DMSO). Growth Conditions: Temperature: 37°C, 5% CO₂: none.

Culture Medium

Excell 610 HSF (Sigma 14610C) + 10% FBS (Gibco 10099141), source-Australia + 1% Glutamax (Gibco 35050).

Growth Mode

Suspension

Additional Info

Depositor

Dr. Dion Daniels, GlaxoSmithKline, (BPMD 5F111), Gunnels Wood Road, Stevenage, Hertfordshire, SG1 2NY.

Country of Origin

United Kingdom

Applications

Produces a specific mouse IgG1 mAb to a protein central to the study of Chronobiology. mAb (6F05-2) detects recombinant and native Rev-Erb alpha protein in ELISA, Western Blot & IP.

Hazard Group (ACDP)

2

References

References

Circadian clock component REV-ERBa controls homeostatic regulation of pulmonary inflammation. J Clin Invest. 2018 Jun 1;128(6):2281-2296. PMID: 29533925REV-ERBa couples the circadian clock to hepatic glucocorticoid action. J Clin Invest. 2018 Oct 1;128(10):4454-4471. PMID: 30179226

Available Formats

  • Frozen

If use of this culture results in a scientific publication, it should be cited in the publication as: Rev-Erb alpha (NR1D1) (ECACC 19061801).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.