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NIMP-R14

NIMP-R14

Catalogue No.

18071701

Cell Line Name

NIMP-R14

Cell Line Description

The cell line is a rat hybridoma (immunogenized by purified BALB/c mouse neutrophils) which produces Anti-RTN4IP1 antibodies, subclass IgG2b.

General Info

Fusion Species

Rat x Rat Hybridoma

Target (Reactivity)

Restricted to haemopoietic cells: neutrophils, eosinophils and macrophages

Unique to ECACC

Yes

Release Conditions

Restricted - commercial organisations are required to complete the 'Cell Line Release Authorisation for Research Use in Commercial Organisations' release conditions form in the supporting documents section.

Characteristics

Myeloma

210RCY3-Ag123

Immunogen

Purified BALB/c mouse neutrophils

Immunological Donor

Rat spleen

Antibody Isotype

IgG2b

Morphology

Spherical

Tissue of Origin

Hybrid

Culture Conditions

Cell Type

Hybridoma

Subculture Routine

Maintain cultures between 3-9 x 10⁵ cells/ml for optimal growth in 5% CO₂/95% air at 37°C.

Culture Medium

DMEM + 20% FBS + L-glutamine

Growth Mode

Suspension

Additional Info

Depositor

Hugh Spotswood on behalf of MRCT Life Arc Address: MRCT/LifeArc, Lynton House, 7-12 Tavistock Square, London, WC1H 9LT, UK Tel +44 (0)20 7391 2801

Country of Origin

United Kingdom

Applications

Flow Cytometry, Immunocytochemistry, Immunohistochemistry

Assays

Neutrophils

Hazard Group (ACDP)

2

Available Formats

  • Frozen

If use of this culture results in a scientific publication, it should be cited in the publication as NIMP-R14 (ECACC 1807101)

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.