HLA Typed Detail

ECACC HLA-Typed Collection: WT49


Supplied by:	European Collection of Authenticated Cell Cultures (ECACC)
Culture Type:	Cell line
Collection:	ECACC HLA-Typed Collection

Catalogue No.:	86052108
Cell Line Name:	WT49
IHW Number:	9285

Citation Guidance:	If use of this culture results in a scientific publication, it should be cited in the publication as: WT49 (ECACC 86052108)
Keywords:	Human HLA-Typed Lymphoblastoid Cell Line

Cell Line Description:	An Epstein-Barr virus (EBV) transformed lymphoblastoid cell line with Human Leukocyte Antigen (HLA)-typing data available.

Species:	Human
Tissue of Origin:	Blood

Ethnic Origin*:	Italian
Homozygous:	Y
WS Region:	USA
Lab Code:	DUQ

CellType:	Lymphoblastoid
Morphology:	Spherical
Growth Mode:	Suspension
GMO Status:	Genetically Modified Organism Class 1 (GMO1)
Genetic Modification:	This is an Epstein-Barr Virus (EBV) transformed cell line
Hazard Group (ACDP):	1
Biosafety Information:	Unless specified otherwise, at the European Collection of Authenticated Cell Cultures (ECACC) we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines. ACDP = Advisory Committee on Dangerous Pathogens (UK) All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises. Hyperlinks to MSDS documents: Frozen cell cultures Material Safety Data Sheet Growing cell cultures Material Safety Data Sheet Nucleic acids derived from cell cultures Material Safety Data Sheet
Subculture Routine:	When resuscitating from frozen, cells should be seeded at 0.5 x 10⁶ cells/ml in the culture medium. The culture should establish log phase growth after 3-5 days. When growing well dilute every 1-3 days to 3 x 10⁵ cells/ml. Maintain cultures between 3-9 x 10⁵ cells/ml for maximum growth; 5% CO₂; 37°C. Cells form floating aggregates of varying size. Protocol: resuscitation of frozen cells
Culture Medium:	RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS). To help establish a culture the concentration of FBS can be increased to 20%.
Release Conditions:	Frozen and growing culture can only be supplied to academic and not for profit organisations. Nucleic acids can be supplied to all researchers including those from commercial organisations.
*Information as provided at time of deposit of the cell line into the ECACC HLA-Typed Collection.
Serological Profile: HLA Class I & Class II Antigens
HLA A:	2
HLA B:	58
Bw4/bw6:	4
HLA Dw:	3
HLA DR:	17
HLA Dw2:	25
HLA DR52:	52
HLA DQ:	2
HLA DP:	4
DNA Profile: HLA Class I & Class II Antigens
A*:	205
Cw*:	701
B*:	5801
HLA DRB1*:	301
HLA DRB3*:	202
HLA DQA1*:	501
HLA DQB1*:	201
HLA DPB1*:	401

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.