Culture Collections

General Cell Collection Detail





ECACC General Cell Collection: GP5d

Supplied by: European Collection of Authenticated Cell Cultures (ECACC)
Culture Type: Cell line
Collection: ECACC General Collection
Catalogue No.: 95090715
Cell Line Name: GP5d
Citation Guidance: If use of this culture results in a scientific publication, it should be cited in the publication as: GP5d (ECACC 95090715)
Keywords: Human Caucasian colon adenocarcinoma
Cell Line Description: GP5d has been established from the same adenocarcinoma as GP2d (ECACC catalogue no. 95090714). This has been confirmed by STR profiling at ECACC. The cells were derived from a local recurrence of Duke's grade B, poorly differentiated carcinoma of the colon from a 71 year old female at surgical resection. The patient received no preoperative chemo- or radio therapy and had a strong family history of colon cancer with two first degree relatives dying of the disease below the age of 55 years. Both clones possess changes consistent with the usual pattern of tumour progression in colon cancer but are morphologically distinct. An interstitial deletion on chromosome 5 (region 14q to 22q) and an inverted duplication of bands 10q11 and 10q21 has been reported. Both cell lines contain a normal copy of the ki-ras gene and a copy with a transition in codon 12. GP5d cells express relatively more TGF alpha mRNA than GP2d cells. GP5d cells undergo a marked epithelial-mesemchymal transition when treated with EGF.
Species: Human
Tissue of Origin: colon
CellType: Epithelial
Growth Mode: Adherent
Karyotype: Diploid
Biosafety Information: Unless specified otherwise, at the European Collection of Authenticated Cell Cultures (ECACC) we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines. ACDP = Advisory Committee on Dangerous Pathogens (UK)
All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country.

ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

Hyperlinks to MSDS documents:
Frozen cell cultures Material Safety Data Sheet
Growing cell cultures Material Safety Data Sheet
Nucleic acids derived from cell cultures Material Safety Data Sheet
Subculture Routine: Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 5x10,000 to 1x100,000 cells/cm² using 0.05% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Culture Medium: DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Depositor: N Solic, Dr D E Davies, Dr J E Collins, CRC Wessex Medical Oncology Unit, University of Southampton
Originator: Yes
Country: UK
References: Solic N, Collins JE, Richter A, Holt SJ, Campbell I, Alexander P, Davies DE 1995 Two newly established cell lines derived from the same colonic adenocarcinoma exhibit differences in EGF-receptor ligand and adhesion molecule expression. Int J Cancer. 62(1):48-57 PMID: 7601566.
Additional Bibliography: Not specified
Patents: None specified by Depositor
Release Conditions: No

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The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

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