|Supplied by:||European Collection of Authenticated Cell Cultures (ECACC)|
|Culture Type:||Cell line|
|Collection:||ECACC General Collection|
|Cell Line Name:||FTC-133|
|Citation Guidance:||If use of this culture results in a scientific publication, it should be cited in the publication as: FTC-133 (ECACC 94060901)|
|Keywords:||Human follicular thyroid carcinoma, lymph node metastasis|
|Cell Line Description:||
FTC-133 was obtained from a lymph node metastasis of a follicular thyroid carcinoma not the primary tumour as originally reported (Rao AS, Goretzki PE, Köhrle J, Brabant G 2005 Letter Re: Id1 gene expression in hyperplastic and neoplastic thyroid tissues. J Clin Endocrinol Metab. 90(10):5906 PMID: 16207897) from a 42-year-old male. The morphology differs from flat polygonal to spindle formed cells. They retained differentiated thyrocyte function and thyrocyte responsiveness to thyrotropin and local active growth factors. Thyroglobulin immunoreactivity could be shown in the cytoplasm and EGF receptor immunoreactivity could be visualised on the cell membranes. Complex chromosomal changes were detected as well as a p53 mutation.
FTC-133 (ECACC Catalogue number 94060901), FTC-236 (ECACC Catalogue number 06030202) and FTC-238 (ECACC Catalogue number 94060902) were all derived from the same individual, a 42 year old male with follicular thyroid cancer. This has been confirmed by short tandem repeat STR-PCR analysis at ECACC The Y chromosome could not be detected in these cell line by (STR)-PCR analysis. It is a known phenomenon that due to the increased genetic instability of cancer cell lines the Y chromosome can be rearranged or lost resulting in lack of detection. The cell lines are identical to the source provided by the depositor based on the STR-PCR analysis. >
|Tissue of Origin:||Thyroid (lymph node metastasis)|
|Karyotype:||Complex chromosomal changes|
Unless specified otherwise, at the European Collection of Authenticated Cell Cultures (ECACC) we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines. ACDP = Advisory Committee on Dangerous Pathogens (UK)
All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country.
ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.
Hyperlinks to MSDS documents:
Frozen cell cultures Material Safety Data Sheet
Growing cell cultures Material Safety Data Sheet
Nucleic acids derived from cell cultures Material Safety Data Sheet
|Subculture Routine:||Split sub-confluent cultures (70-80%) 1:8 to 1:12 i.e. seeding at 1-5x10,000 cells/cm² using 0.05% trypsin or trypsin/EDTA; 5% CO2; 37°C.|
|Culture Medium:||DMEM:Ham's F12 (1:1) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).|
|Depositor:||Dr J Köhrle, Klinische Forschergruppe, Medizinische Poliklinik, Würzburg|
|References:||Goretzki PE et al., Front Horm Res 1989;18:56-80|
|Additional Bibliography:||Hölting T, Zielke A, Siperstein AE, Clark OH, Duh QY 1994 Transforming growth factor-beta 1 is a negative regulator for differentiated thyroid cancer: studies of growth, migration, invasion, and adhesion of cultured follicular and papillary thyroid cancer cell lines. J Clin Endocrinol Metab. 79(3):806-13 PMID: 8077365. Kebebew E, Peng M, Treseler PA, Clark OH, Duh QY, Ginzinger D, Miner R 2004 Id1 gene expression is up-regulated in hyperplastic and neoplastic thyroid tissue and regulates growth and differentiation in thyroid cancer cells. J Clin Endocrinol Metab. 89(12):6105-11.PMID: 15579766. Barretina J, et al., 2012 The Cancer Cell Line Encyclopedia enables predictive modelling of anticancer drug sensitivity. Nature. 483(7391):603-7. PMID: 22460905.|
|Patents:||None specified by Depositor|
The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.
Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.
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