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UK Health Security Agency

THP 1

THP 1

Catalogue No.

88081201

Cell Line Name

THP 1

Cell Line Description

Derived from the peripheral blood of a 1 year old male with acute monocytic leukaemia. THP-1 cells have Fc and C3b receptors and lack surface and cytoplasmic immunoglobulins. These cells also stain positive for alpha-napthhyl butyrate esterase, produce lysozymes and are phagocytic (both latex beads and sensitised erythrocytes). THP-1 cells can also restore the response of purified T lymphocytes to Concanavlin A, show increased CO₂ production on phagocytosis and can be differentiated into macrophage-like cells using for example DMSO.

 

Please note: Our ECACC cell culturists routinely resuscitate and culture the THP 1 cell line using the protocol given in the Subculture routine section of this catalogue entry. If you require further assistance, please contact our Technical Support team.

Characteristics

Receptors

Fc, C3b

Tissue of Origin

Blood

DNA profile (STR Profile)

Amelogenin: X,Y
CSF1PO: 11,13
D5S818: 11,12
D7S820: 10
D13S317: 13
D16S539: 11,12
TH01: 8,9.3
TPOX: 8,11
vWA: 16

Karyotype

Not specified

Disease

Monocytic Leukaemia

Culture Conditions

Cell Type

Monocyte

Subculture Routine

If starting from a frozen ampoule the cryoprotectant should be removed. Add thawed cells to a conical based centrifuge tube e.g. 15ml tube, slowly add 4 ml of culture medium to the tube. Take a sample of the cell suspension, e.g. 100μl, to count cells. Centrifuge the cell suspension at low speed i.e. 100 - 150 x g for a maximum of 5 minutes. Remove medium and resuspend the cell pellet at a density of 3 - 5 x 100,000 cells/ml in fresh medium containing 20% serum. Incubate flask at 37°C; 5 - 7% CO2. Check daily. Keep flask in a vertical position until the cells reach the exponential phase of growth. This can take up to 7 days. Once the culture is established the serum concentration can be reduced to 10%. To keep the cells in exponential growth, maintain cultures between 3-8x100,000 cells/ml. Requires 5% DMSO and 95% foetal bovine serum (FBS) as cryoprotectant.

Culture Medium

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Growth Mode

Suspension

Additional Info

Depositor

Dr J Clarke, AVRI, Pirbright

Country of Origin

United Kingdom

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

Int J Cancer 1980;26:171; Cancer Res 1982;42:1530; J Immunol 1983;131:1882

Available Formats

  • Frozen

If use of this culture results in a scientific publication, it should be cited in the publication as: THP 1 (ECACC 88081201).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.