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Jurkat E6.1

Jurkat E6.1

Catalogue No.

88042803

Cell Line Name

Jurkat E6.1

Cell Line Description

Derived from Jurkat FHCRC. An IL-2 producing cell line, derived by incubating the cells at 41°C for 48 hours followed by a limiting dilution cloning over macrophages.

Characteristics

Products

Interleukin 2 (IL-2), human alpha interferon

Tissue of Origin

Blood

DNA profile (STR Profile)

Amelogenin: X,Y
CSF1PO: 11,12
D5S818: 9
D7S820: 8,10
D13S317: 8,12
D16S539: 11
TH01: 6,9.3
TPOX: 8,10
vWA: 18

Karyotype

Pseudodiploid, modal No 46

Applications

IL-2 assay and cloning studies

Disease

Lymphoblastic Leukaemia

Culture Conditions

Cell Type

Lymphoblastoid

Subculture Routine

Maintain cultures between 3-9x100,000 cells/ml; 5% CO₂; 37°C. On resuscitation single cells can be observed, during culture most cells will grow as aggregates.

Culture Medium

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Growth Mode

Suspension

Additional Info

Depositor

Prof H Harris/Dr C Madin, Sir William Dunn School of Pathology, Oxford

Country of Origin

United Kingdom

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

J Immunol 1984;133:123; J Immunol Meth 1993;157:203-207

Bibliography

Not specified

Available Formats

  • Frozen

If use of this culture results in a scientific publication, it should be cited in the publication as: Jurkat E6.1 (ECACC 88042803).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.