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UK Health Security Agency

AtT20

AtT20

Catalogue No.

87021902

Cell Line Name

AtT20

Cell Line Description

Derived after alternate passage of LAF1 mouse pituitary tumour cells as tumours in animals and in cell culture. Cells produce adrenocorticotropic hormone (ACTH) at approximately the rate of 1000mU/mg of cell protein per week.

Characteristics

Products

Adrenocorticotropic hormone (ACTH)

Tissue of Origin

Pituitary gland

Morphology

Small round cells growing as large aggregates

Karyotype

Diploid

Applications

Hormone studies

Disease

Neoplasms

Culture Conditions

Cell Type

Epithelial

Subculture Routine

This cell line loses viability when frozen and hence the expected viable cell count when resuscitated from frozen stock may be only 40 -50%. To sub-culture allow clusters to settle and remove all but 5 to 10 ml of medium. Gently transfer the clusters to new flasks and add fresh medium. A subcultivation ratio of 1:2 is recommended. Cells grow in large viable clusters making counting difficult. A seeding density of approximately 3 x 10⁵/ml is recommended. 5% CO₂; 37°C. Proliferation is enhanced by incubation on a shaking platform.

Culture Medium

Ham's F10 + 2mM Glutamine + 15% Horse Serum (HS) + 2.5% Foetal Bovine Serum (FBS). Cells grow in large aggregates.

Growth Mode

Aggregates in suspension

Additional Info

Depositor

Obtained from ATCC

Country of Origin

United States

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

FURTH J, GADSEN EL, UPTON AC 1953 ACTH secreting transplantable pituitary tumors. Proc Soc Exp Biol Med. 84(1):253-4 PMID: 13121002.

Bibliography

BUONASSISI V, SATO G, COHEN AI.1962 Hormone-producing cultures of adrenal and pituitary tumor origin. Proc Natl Acad Sci U S A. 48:1184-90 PMID: 13874682.

Available Formats

  • Frozen

If use of this culture results in a scientific publication, it should be cited in the publication as: AtT20 (ECACC 87021902).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.