Culture Collections

General Cell Collection Detail





ECACC General Cell Collection: HCT 116 p300 KO Rescue

Supplied by: European Collection of Authenticated Cell Cultures (ECACC)
Culture Type: Cell line
Collection: ECACC General Collection
Catalogue No.: 17011211
Cell Line Name: HCT 116 p300 KO Rescue
Organisation deposited by: Ximbio
Other Collection No.: No
Unique to ECACC: Yes
Citation Guidance: If use of this culture results in a scientific publication, it should be cited in the publication as: HCT 116 p300 KO Rescue (ECACC 17011211)
Keywords: p300 Knock-Out, colon carcinoma model, HCT 116, p53-dependent apoptosis, cellular adhesion and migration.
Cell Line Description: The cell line HCT 116 is hemizygous for p300 and is expressed from a single allele. p300 knockout cells were obtained by targeting exon 2 of the EP300 gene using the targeting construct pIRESbeta-Geo-p300KO. Rescue clones were obtained by co-transfecting pcDEF Flag-p300 expression vector with pPGK-Puromycin construct, as described by Iyer et al. 2004.
Species: Human
Tissue of Origin: Colon
Disease Status: Cancer
Donor Sex: Male
CellType: Epithelial
Morphology: Polygonal epithelium
Growth Mode: Adherent
DNA Profile: DNA Profile (STR Analysis):

Amelogenin: X X

CSF1PO: 7 10

D13S317: 10 13

D16S539: 11 13.

D18S51: 16 17

D21S11: 29 30

D3S1358: 12 17

D5S818: 10 11

D7S820: 11 12

D8S1179: 11 14

FGA: 18 23

Penta D: 9 13

Penta E: 13 14

TH01: 8 9

TPOX: 8 8

vWA: 18 22

The Y chromosome could not be detected in this cell line by short tandem repeat (STR)-PCR analysis when tested at ECACC. It is a known phenomenon that due to the increased genetic instability of cancer cell lines the Y chromosome can be rearranged or lost resulting in lack of detection. The cell line is identical to the source provided by the depositor based on the STR-PCR analysis.

GMO Status: GMO1
Genetic Modification: p300 knockout cells were obtained by targeting exon 2 of the EP300 gene using the targeting construct pIRESbeta-Geo-p300KO. Rescue clones were obtained by cotransfecting pcDEFFlag-p300 expression vector with pPGK-Puromycin construct.
Hazard Group (ACDP): 2
Biosafety Information: Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK)
All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country.

ACDP Guidance

Hyperlinks to Material Safety Data Sheets (MSDS) documents:
Frozen cell cultures MSDS
Growing cell cultures MSDS
Nucleic acids derived from cell cultures MSDS
Subculture Routine: Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x104 cells/cm² using 0.05% trypsin/EDTA solution. Cells are cultured at 37°C, in 95% air and 5% CO2, and passaged every 3 to 4 days

Culture Medium: McCoy's 5a medium modified, with 10% foetal bovine serum and 2mM L-Glutamine
Depositor: Ximbio (formerly known as Cancer Research Technologies Limited)
Originator: Yes
Country: UK
References: Iyer et al. 2004. Proc Natl Acad Sci U S A. 101(19):7386-91. PMID: 15123817
Krubasik et al. 2006. Br J Cancer. 94(9):1326-32. PMID: 16622451
Bundy et al. 2006. Cancer Res. 66(15):7606-14. PMID: 16885360.
Iyer et al. 2007. Oncogene. 26(1):21-9. PMID: 16878158.
Additional Bibliography: Wallace et al. 2015. PLoS Pathog. 11(3):e1004687. PMID: 25803638.
Patents: None
Release Conditions: Restricted - commercial organisations are required to complete the 'Cell Line Release Authorisation for Research Use in Commercial Organisations' release conditions form in the supporting documents section.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

Available Formats

Frozen
DNA-5ug (100ng/ul)

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