|Supplied by:||European Collection of Authenticated Cell Cultures (ECACC)|
|Culture Type:||Cell line|
|Collection:||ECACC General Collection|
|Cell Line Name:||ES-E14TG2a|
|Citation Guidance:||If use of this culture results in a scientific publication, it should be cited in the publication as: ES-E14TG2a (ECACC 08021401)|
|Keywords:||Mouse (strain 129/Ola) embryonic stem cell|
|Cell Line Description:||Pluripotent mouse embryonic stem cell line. ES-E14TG2a cells are deficient in in HPRT and are resistant to 0.06mM 6-thioguanine.|
|Tissue of Origin:||Embryo blastocyst|
|CellType:||Adherent monolayer of spheroidal cells on feeder layer of mouse primary embryonic fibroblasts or STO cells ECACC Catalogue 86032003 (irradiated or treated with mitomycin C to prevent cell division. The cells spontaneously differentiate and form embryonal structures in the absence of a feeder layer.|
Unless specified otherwise, at the European Collection of Authenticated Cell Cultures (ECACC) we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines. ACDP = Advisory Committee on Dangerous Pathogens (UK)
All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country.
ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.
Hyperlinks to MSDS documents:
Frozen cell cultures Material Safety Data Sheet
Growing cell cultures Material Safety Data Sheet
Nucleic acids derived from cell cultures Material Safety Data Sheet
|Culture Medium:||DMEM + 2mM Glutamine + 1% NEAA + 0.1mM 2-Mercaptoethanol + 1000U/ml mouse inhibitory factor (LIF) + 1.5% sodium bicarbonate + 10% Foetal Bovine Serum. Renewal of medium every 2-3 days is required.|
|Depositor:||Professor T Doetschman, Institute for Collaborative Research, University of Arizona, Tucson, Arizona 85721-0036. Originator: Professor M Hooper, University of Edinburgh EH4 2XU, UK.|
|References:||Smith & Hooper (1987) Buffalo rat liver cells produce a diffusible activity which inhibits the differentiation of murine embryonal carcinoma and embryonic stem cells. Dev. Biol. 121: 1-9. PMID: 3569655.|
|Additional Bibliography:||Kuehn et al., (1987) A potential animal model for Lesch-Nyhan syndrome through introduction of HPRT mutations into mice. Nature 326: 295-8. PMID: 3029599.Doetschman et al., (1987) Targetted correction of a mutant HPRT gene in mouse embryonic stem cells. Nature 330: 576-578. PMID: 3683574|
|Patents:||None specified by depositor|
The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.
Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.
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