CHO-K1.Cl-0204
CHO-K1.Cl-0204
Cell Line Name
Cell Line Description
Clone of CHO-K1 cells expressing human FcγRIIa of the 131R allotype asa transmembrane protein with its native intracellular domain
General Info
Species
Hamster
Unique to ECACC
Links
Cellosaurus: CVCL_DE93
Release Conditions
Characteristics
Receptors
Products
Tissue of Origin
Morphology
Karyotype
Applications
Measurement of IgG binding
Disease
Culture Conditions
Cell Type
Subculture Routine
Split sub-confluent cultures (70-80%) 1:3 to 1:6. Seeding at 2-5 x 10,000 cells/cm² using 0.05% trypsin/EDTA; culture conditions 5% CO₂/95% air; 37°C
Culture Medium
EMEM (EBSS) + 2mM Glutamine + 1% Non- Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS) with 600ug/ml hygromycin B
Growth Mode
Additional Info
Depositor
Country of Origin
GMO Status
Genetic Modification
Stably transfected with a pcDNA3.1/Hygro(+) based vector with a human FCγRIIIa 131R cDNA insert that is expressed as a transmembrane protein with its native intracellular domain
Hazard Group (ACDP)
Applications
References
Armour KL, Smith CS, Turner CP, Kirton CM, Wilkes AM, Hadley AG, Ghevaert C, Williamson LM, Clark MR. Low-affinity Fc gamma R interactions can decide the fate of novel human IgG-sensitised red blood cells and platelets. Eur J Immunol. 2014Mar; 44 (3):905-14. doi: 10.1002/eji.201343825. Epub 2014 Feb 16. PubMed
Bibliography
Armour KL, Smith CS, Ip NC, Ellison CJ, Kirton CM, Wilkes AM, Williamson LM, Clark MR. Clearance of human IgG1-sensitised red blood cells in vivo in humans relates to the in vitro properties of antibodies from alternative cell lines. PLoS One. 2014 Oct 10;9(10):e109463. doi: 10.1371/journal.pone.0109463. eCollection 2014. PubMed PMID: 25302805; PubMed Central PMCID: PMC4193810. PMID:24285214; PubMed Central PMCID: PMC4209800.
Available Formats
- Frozen
- DNA-5µg (100ng/µl)
Citation Guidance
If use of this culture results in a scientific publication, it should be cited in the publication as: CHO-K1.Cl-0204 (ECACC 15042903).
Biosafety Information
Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country.
ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.
Further Information
The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.
Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.