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Bacteria Collection: Streptococcus dysgalactiae subsp. equisimilis

NCTC Number: NCTC 8543
Current Name: Streptococcus dysgalactiae subsp. equisimilis
Original Strain Reference: 655 Chestle
Other Collection No: ATCC 35666; CHESTLE; DSM 6176; 655
Previous Catalogue Name: streptococcus equisimilis
Type Strain: No
Family: Streptococcaceae
Hazard Group (ACDP): 2
Release Restrictions: Terms & Conditions of Supply of Microbial Pathogens: Safety
Antigenic Properties: serovar group c
Conditions for growth on solid media: Columbia blood agar, 24-48 hours, 37°C, aerobic
Conditions for growth on liquid media: nutrient broth,37, facultative anaerobe
Isolated From: human, human vagina
Whole Genome Sequence: http://www.ebi.ac.uk/ena/data/view/ERS1143232
16S rRNA Gene Sequence: >gb|AB096755|ATCC 35666|Streptococcus dysgalactiae subsp. equisimilis gene for 16S rRNA,partial sequence, strain:ATCC 35666.| gctggcggcgtgcct... >gb|AY121360|ATCC 35666|Streptococcus dysgalactiae subsp. equisimilis strain ATCC 35666 16Sribosomal RNA gene, partial sequence.| tggaaacgatagcta... >gb|AJ319643|ATCC 35666|Streptococcus dysgalactiae subsp. equisimilis partial 16S rRNAgene, strain ATCC 35666.| gaacgctggcggcgt... >gb|AB258407|ATCC 35666|Streptococcus dysgalactiae subsp. equisimilis DNA, 16S-23S rRNAintergenic spacer, partial sequence.| gagaggtcttgtggg...
23S rRNA Gene Sequence: >gb|AB096742|ATCC 35666|Streptococcus dysgalactiae subsp. equisimilis gene for 23S rRNA,complete sequence, strain:ATCC 35666.| ggttaagttaataag... >gb|AB258407|ATCC 35666|Streptococcus dysgalactiae subsp. equisimilis DNA, 16S-23S rRNAintergenic spacer, partial sequence.| gagaggtcttgtggg...
Bibliography: TODD E W 1939 J.HYG.,CAMB. 39 1
Extended Bibliography: showhide Show bibliography
Ref #: 83026
Author(s): Woo,P.C.;Teng,J.L.;Lau,S.K.;Lum,P.N.;Leung,K.W.;Wong,K.L.;Li,K.W.;Lam,K.C.;Yuen,K.Y.
Journal: J Clin Microbiol
Title: Analysis of a viridans group strain reveals a case of bacteremia due to lancefield group G alpha-hemolytic Streptococcus dysgalactiae subsp equisimilis in a patient with pyomyositis and reactive arthritis
Volume: 41
Page(s): 613-8
Year: 2003
Keyword(s): Aged *Antigens, Bacterial Arthritis, Reactive/*complications/microbiology Bacteremia/*microbiology Bacterial Outer Membrane Proteins/genetics Bacterial Proteins/classification/genetics Carrier Proteins/genetics Chaperonins Escherichia coli Proteins Female Heat-Shock Proteins/classification/genetics Humans Phenotype RNA, Ribosomal, 16S/analysis/classification/genetics Streptococcus/classification/*isolation & purification
Remarks: Streptococcus dysgalactiae is classified by a combination of phenotypic and genotypic characteristics into Lancefield group C alpha-hemolytic Streptococcus dysgalactiae subsp. dysgalactiae and Lancefield group C, group G, and group L beta-hemolytic Streptococcus dysgalactiae subsp. equisimilis. In this study, we report the isolation of a catalase-negative, alpha-hemolytic, optochin- and bacitracin-resistant viridans group strain, which does not grow in 10 or 40% bile, on MacConkey agar or bile esculin agar, or in 6% NaCl, from the blood culture of a 73-year-old woman with pyomyositis and poststreptococcal reactive arthritis. Lancefield grouping revealed that the strain was a group G streptococcus. The Vitek system (GPI) showed that it was unidentified, and the API system (20 STREP) showed that it was 95.7% S. dysgalactiae subsp. dysgalactiae. 16S rRNA gene sequencing showed that it was a strain of S. dysgalactiae. Based on phylogenetic affiliation with 16S rRNA gene or GroEL amino acid (another bacterial gene, in addition to 16S rRNA gene, that is highly conserved) sequences, the strain is most closely related to Lancefield group C beta-hemolytic S. dysgalactiae subsp. equisimilis. PCR amplification and sequencing of the streptolysin S structural gene (sagA) and M protein gene (emm) hypervariable region showed the presence of these suspected primary virulence factors. Further studies would delineate whether the isolate is just a hemolysin-deficient variant of group G beta-hemolytic S. dysgalactiae subsp. equisimilis or a novel type of S. dysgalactiae. The present case showed that group G alpha-hemolytic S. dysgalactiae subsp. equisimilis can be associated with serious invasive infection and poststreptococcal sequelae.
URL: 12574255
Ref #: 83019
Author(s): Kawata,K.;Minakami,T.;Mori,Y.;Katsumi,M.;Kataoka,Y.;Ezawa,A.;Kikuchi,N.;Takahashi,T.
Journal: Int J Syst Evol Microbiol
Title: rDNA sequence analyses of Streptococcus dysgalactiae subsp. equisimilis isolates from pigs
Volume: 53
Page(s): 1941-6
Year: 2003
Keyword(s): Animals Base Sequence DNA Primers DNA, Bacterial/genetics DNA, Ribosomal/*genetics *Phylogeny RNA, Bacterial/genetics RNA, Ribosomal, 16S/genetics RNA, Ribosomal, 23S/genetics Sequence Alignment Sequence Homology, Nucleic Acid Streptococcus/*classification/*genetics/isolation & purification Swine/*microbiology
Remarks: The nucleotide sequences of 16S and 23S rRNA genes (rDNA) were determined for 11 isolates of Streptococcus dysgalactiae subsp. equisimilis from slaughtered pigs with endocarditis, arthritis or lymphadenitis and strain ATCC 35666, designated as a strain of subspecies equisimilis. The sequences of each of the genes were compared phylogenetically with the corresponding sequences of S. dysgalactiae subsp. dysgalactiae ATCC 43078(T) and ATCC 27957, which were also determined in this study. Based on the 16S rDNA analysis, the isolates of S. dysgalactiae subsp. equisimilis were divided into two distinct groups, designated groups 1 and 2. S. dysgalactiae subsp. equisimilis ATCC 35666 was closely related to the group 2 strains. The S. dysgalactiae subsp. dysgalactiae strains seemed to be associated with the group 1 strains, which was not consistent with the conventional subspecific classification of S. dysgalactiae. In contrast, the 23S rDNA analysis distinguished S. dysgalactiae subsp. dysgalactiae strains from subsp. equisimilis strains. This inconsistency between phylogenies based on 16S and 23S rDNA indicates that 23S rDNA is a more rigid marker for determining the phylogenetic relationships and taxonomic position of these organisms than is 16S rDNA.
URL: 14657127
Data: R. M. Fry, PHLS Cambridge in 1953 / Vagina / Preparation of group C antiserum / Todd, E. W. (1939) J. Hyg., Camb. 39, 1
Accession Date: 01/01/1953
History: RECEIVED FRY R M ,P.H.L.S. CAMBRIDGE
Depositor: FRY R M
Taxonomy: TaxLink: ? (NCTC 8543) - Date of change: 04/02/2003
Biosafety Responsibility: It is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country

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The Culture Collections hold cell cultures, bacteria, fungi and virus strains from worldwide sources. Our scientists ensure that the identification of the cultures is correct and they remain unchanged from when they are first deposited with the Collection. Nevertheless, some of the data we provide about the cultures is supplied by the person depositing the strains and, although we have multiple checking procedures in place, we cannot always verify all their data. Please note that the Culture Collections cannot be held responsible for any inaccuracies in the data provided by the depositors.

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