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Bacteria Collection: Staphylococcus aureus

NCTC Number: NCTC 8532
Current Name: Staphylococcus aureus
Original Strain Reference: S33 R4
Other Collection No: ATCC 12600; CCM 885; DSM 20231; NCDO 949
Previous Catalogue Name: Staphylococcus aureus
Type Strain: Yes
Family: Staphylococcaceae
Hazard Group (ACDP): 2
Release Restrictions: Terms & Conditions of Supply of Microbial Pathogens: Safety
Antigenic Properties: serovar serotype 3
Conditions for growth on solid media: Nutrient agar, 24-48 hours, 37°C, aerobic
Conditions for growth on liquid media: nutrient broth,37, facultative anaerobe
Isolated From: human, pleural fluid containing acid-fast bact.
Whole Genome Sequence: https://www.ebi.ac.uk/ena/data/view/ERS445052
Annotated Genome: ftp://ftp.sanger.ac.uk/pub/project/pathogens/NCTC3000/d...
16S rRNA Gene Sequence: >gb|D83357|ATCC12600T|Staphylococcus aureus (strain ATCC12600T) gene for 16S rRNA,partial sequence.| aggatgaacgctggc... >gb|X68417|ATCC 12600|S.aureus gene for 16S rRNA.| ttttatggagagttt... >gb|X70648|NCDO 949|S.aureus 16S rRNA (partial).| tgaacgctggcggcg... >gb|AY688035|DSM 20231|Staphylococcus aureus subsp. aureus strain DSM 20231 16S ribosomalRNA gene, partial sequence.| catgcaagtcgagcg...
23S rRNA Gene Sequence: >gb|X68425|ATCC 12600|S.aureus gene for 23S rRNA.| gattaagttattaag...
Miscellaneous Sequence Data: >gb|AB084060|ATCC12600|Staphylococcus aureus gyrB gene for gyrase B, partial cds,strain:ATCC12600.| cggtggcggatacaa...
Bibliography: HOBBS B C 1942 J HYG CAMB 46 222;COWAN S T 1954 J PATH BACT 48 169; COWAN
Extended Bibliography: showhide Show bibliography
Ref #: 80967
Author(s): Bentley,R.W.;Harland,N.M.;Leigh,J.A.;Collins,M.D.
Journal: Lett Appl Microbiol
Title: A Staphylococcus aureus-specific oligonucleotide probe derived from 16S rRNA gene sequences
Volume: 16
Page(s): 203-6
Year: 1993
Keyword(s): Base Composition Base Sequence Blotting, Northern Genes, Bacterial Molecular Sequence Data Nucleic Acid Hybridization *Oligonucleotide Probes/chemistry RNA, Bacterial/chemistry/*genetics RNA, Ribosomal, 16S/*genetics/isolation & purification Sequence Alignment Sequence Homology, Nucleic Acid Species Specificity Staphylococcus/genetics Staphylococcus aureus/*genetics/isolation & purification
Remarks: Genes encoding 16S rRNA were sequenced from 16 species of Staphylococcus. Sequence analysis highlighted a potential Staph. aureus-specific region and a complementary oligonucleotide probe was synthesized and its specificity tested. Northern blotting indicated molecular specificity, and dot blots to RNA from Staph. aureus, Staph. capitis, Staph. caprae, Staph. carnosus, Staph. caseolyticus, Staph. cohnii, Staph. epidermidis, Staph. gallinarum, Staph. haemolyticus, Staph. hominis, Staph. hyicus, Staph. saprophyticus, Staph. sciuri, Staph. simulans, Staph. warneri and Staph. xylosus indicated species-specificity.
URL: 7683195
Ref #: 95528
Author(s): Hopewell,R.;Oram,M.;Briesewitz,R.;Fisher,L.M.
Journal: J Bacteriol
Title: DNA cloning and organization of the Staphylococcus aureus gyrA and gyrB genes: close homology among gyrase proteins and implications for 4-quinolone action and resistance
Volume: 172
Page(s): 3481-4
Year: 1990
Keyword(s): GENBANK/M37915 Amino Acid Sequence *Cloning, Molecular DNA Topoisomerases, Type II/*genetics DNA, Bacterial/*analysis Drug Resistance, Microbial/genetics *Genes, Bacterial Molecular Sequence Data Quinolones/*pharmacology Staphylococcus aureus/drug effects/*genetics
Remarks: Staphylococcus aureus gyrA and gyrB genes, which encode the DNA gyrase A and B proteins, have been isolated and found to map contiguously. DNA sequence analysis revealed close homology between the S. aureus gyrase subunits and their counterparts in Bacillus subtilis and Escherichia coli, including several conserved amino acid residues whose substitution in E. coli confers resistance to 4-quinolones. These results are discussed in regard to quinolone resistance mechanisms in S. aureus.
URL: 2160946
Ref #: 95529
Author(s): Ito,H.;Yoshida,H.;Bogaki-Shonai,M.;Niga,T.;Hattori,H.;Nakamura,S.
Journal: Antimicrob Agents Chemother
Title: Quinolone resistance mutations in the DNA gyrase gyrA and gyrB genes of Staphylococcus aureus
Volume: 38
Page(s): 2014-23
Year: 1995
Keyword(s): GENBANK/D10489 Amino Acid Sequence Anti-Infective Agents/*pharmacology Bacterial Proteins/*genetics Base Sequence Cloning, Molecular DNA Gyrase DNA Topoisomerases, Type II/*genetics DNA, Bacterial/*genetics Drug Resistance, Microbial/genetics Genes, Bacterial Genetic Complementation Test Molecular Sequence Data *Mutation Oxolinic Acid/pharmacology Sequence Homology, Amino Acid Staphylococcus aureus/enzymology/*genetics Transformation, Bacterial
Remarks: A 6.4-kb DNA fragment containing the DNA gyrase gyrA and gyrB genes was cloned and sequenced from the quinolone-susceptible Staphylococcus aureus type strain ATCC 12600. An expression plasmid was constructed by inserting the cloned genes into the Escherichia coli-S. aureus shuttle vector pAT19, and deletion plasmids carrying only functional gyrA and gyrB genes were derived from this plasmid. An efficient transformation system for S. aureus RN4220 was established by using these plasmids. Quinolone-resistant mutants of S. aureus RN4220 were isolated by three-step selection with quinolones. The first- and second-step mutants were considered to be transport mutants, and the third-step mutants were divided into five groups with respect to their resistance patterns and transformation results with gyrA and gyrB genes. Sequencing analysis of the resulting mutant gyrase genes showed that they had the following point mutations: group 1, Ser-84 (TCA) to Leu (TTA) in GyrA; group 2, Ser-84 (TCA) to Ala (GCA), Ser-85 (TCT) to Pro (CCT), or Glu-88 (GAA) to Lys (AAA) in GyrA; group 3, Asp-437 (GAC) to Asn (AAC) in GyrB; group 4, Arg-458 (CGA) to Gln (CAA) in GyrB; and group 5, Ser-85 (TCT) to Pro (CCT) in GyrA and Asp-437 (GAC) to Asn (AAC) in GyrB. When the gyrA and/or gyrB mutants were transformed with the wild-type gyrA and/or gyrB plasmids, they became quinolone susceptible, but transformants with the plasmids having the same mutations on the gyrA and/or gyrB genes did not confer susceptibility. These results indicate that mutations in both gyrA and gyrB can be responsible for quinolone resistance in S. aureus.
URL: 7811012
Ref #: 81199
Author(s): Becker,K.;Harmsen,D.;Mellmann,A.;Meier,C.;Schumann,P.;Peters,G.;von Eiff,C.
Journal: J Clin Microbiol
Title: Development and evaluation of a quality-controlled ribosomal sequence database for 16S ribosomal DNA-based identification of Staphylococcus species
Volume: 42
Page(s): 4988-95
Year: 2004
Keyword(s): GENBANK/AY688029 GENBANK/AY688030 GENBANK/AY688031 GENBANK/AY688032 GENBANK/AY688033 GENBANK/AY688034 GENBANK/AY688035 GENBANK/AY688036 GENBANK/AY688037 GENBANK/AY688038 GENBANK/AY688039 GENBANK/AY688040 GENBANK/AY688041 GENBANK/AY688042 GENBANK/AY688043 GENBANK/AY688044 GENBANK/AY688045 GENBANK/AY688046 GENBANK/AY688047 GENBANK/AY688048 GENBANK/AY688049 GENBANK/AY688050 GENBANK/AY688051 GENBANK/AY688052 GENBANK/AY688053 GENBANK/AY688054 GENBANK/AY688055 GENBANK/AY688056 GENBANK/AY688057 GENBANK/AY688058 GENBANK/AY688059 GENBANK/AY688060 GENBANK/AY688061 GENBANK/AY688062 GENBANK/AY688063 GENBANK/AY688064 GENBANK/AY688065 GENBANK/AY688066 GENBANK/AY688067 GENBANK/AY688068 GENBANK/AY688069 GENBANK/AY688070 GENBANK/AY688071 GENBANK/AY688072 GENBANK/AY688073 GENBANK/AY688074 GENBANK/AY688075 GENBANK/AY688076 GENBANK/AY688077 GENBANK/AY688078 GENBANK/AY688079 GENBANK/AY688080 GENBANK/AY688081 GENBANK/AY688082 GENBANK/AY688083 GENBANK/AY688084 GENBANK/AY688085 GENBANK/AY688086 GENBANK/AY688087 GENBANK/AY688088 GENBANK/AY688089 GENBANK/AY688090 GENBANK/AY688091 GENBANK/AY688092 GENBANK/AY688093 GENBANK/AY688094 GENBANK/AY688095 GENBANK/AY688096 GENBANK/AY688097 GENBANK/AY688098 GENBANK/AY688099 GENBANK/AY688100 GENBANK/AY688101 GENBANK/AY688102 GENBANK/AY688103 GENBANK/AY688104 GENBANK/AY688105 GENBANK/AY688106 GENBANK/AY688107 GENBANK/AY688108 GENBANK/AY688109 *Bacterial Typing Techniques DNA, Bacterial/analysis DNA, Ribosomal/analysis *Databases, Nucleic Acid Genes, rRNA Humans Molecular Sequence Data RNA, Ribosomal, 16S/*genetics Sequence Analysis, DNA Species Specificity Staphylococcal Infections/*microbiology Staphylococcus/*classification/genetics/metabolism
Remarks: To establish an improved ribosomal gene sequence database as part of the Ribosomal Differentiation of Microorganisms (RIDOM) project and to overcome the drawbacks of phenotypic identification systems and publicly accessible sequence databases, both strands of the 5' end of the 16S ribosomal DNA (rDNA) of 81 type and reference strains comprising all validly described staphylococcal (sub)species were sequenced. Assuming a normal distribution for pairwise distances of all unique staphylococcal sequences and choosing a reporting criterion of > or =98.7% similarity for a "distinct species," a statistical error probability of 1.0% was calculated. To evaluate this database, a 16S rDNA fragment (corresponding to Escherichia coli positions 54 to 510) of 55 clinical Staphylococcus isolates (including those of the small-colony variant phenotype) were sequenced and analyzed by the RIDOM approach. Of these isolates, 54 (98.2%) had a similarity score above the proposed threshold using RIDOM; 48 (87.3%) of the sequences gave a perfect match, whereas 83.6% were found by searching National Center for Biotechnology Information (NCBI) database entries. In contrast to RIDOM, which showed four ambiguities at the species level (mainly concerning Staphylococcus intermedius versus Staphylococcus delphini), the NCBI database search yielded 18 taxon-related ambiguities and showed numerous matches exhibiting redundant or unspecified entries. Comparing molecular results with those of biochemical procedures, ID 32 Staph (bioMerieux, Marcy I'Etoile, France) and VITEK 2 (bioMerieux) failed to identify 13 (23.6%) and 19 (34.5%) isolates, respectively, due to incorrect identification and/or categorization below acceptable values. In contrast to phenotypic methods and the NCBI database, the novel high-quality RIDOM sequence database provides excellent identification of staphylococci, including rarely isolated species and phenotypic variants.
URL: 15528685
Ref #: 13732
Author(s): Bentley,R.W.;Harland,N.M.;Leigh,J.A.;Collins,M.D.
Journal: Lett Appl Microbiol
Title: A Staphylococcus aureus-specific oligonucleotide probe derived from 16S rRNA gene sequences
Volume: 16
Page(s): 203-6
Year: 1993
Keyword(s): Base Composition Base Sequence Blotting, Northern Comparative Study Genes, Bacterial Molecular Sequence Data Nucleic Acid Hybridization *Oligonucleotide Probes/chemistry RNA, Bacterial/chemistry/*genetics RNA, Ribosomal, 16S/*genetics/isolation & purification Sequence Alignment Sequence Homology, Nucleic Acid Species Specificity Staphylococcus/genetics Staphylococcus aureus/*genetics/isolation & purification Support, Non-U.S. Gov't
Remarks: Genes encoding 16S rRNA were sequenced from 16 species of Staphylococcus. Sequence analysis highlighted a potential Staph. aureus-specific region and a complementary oligonucleotide probe was synthesized and its specificity tested. Northern blotting indicated molecular specificity, and dot blots to RNA from Staph. aureus, Staph. capitis, Staph. caprae, Staph. carnosus, Staph. caseolyticus, Staph. cohnii, Staph. epidermidis, Staph. gallinarum, Staph. haemolyticus, Staph. hominis, Staph. hyicus, Staph. saprophyticus, Staph. sciuri, Staph. simulans, Staph. warneri and Staph. xylosus indicated species-specificity.
URL: 93244084
Ref #: 1277
Author(s): Meyer,S.A.;Schleifer,K.H.
Journal: Arch. Microbiol.
Title: Deoxyribonucleic acid reassociation in the classification of coagulase-positive staphylococci.
Volume: 117
Page(s): 183-188
Year: 1978
Ref #: 1300
Author(s): Skerman,V.B.D.;McGowan,V.;Sneath,P.H.A.(ed)
Journal: Int. J. Syst. Bacteriol.
Title: Approved Lists of Bacterial Names.
Volume: 30
Page(s): 225-420
Year: 1980
Ref #: 1332
Author(s): Kilpper-Bälz,R.;Schleifer,K.H.
Journal: FEMS Microbiol. Lett.
Title: DNA-rRNA hybridization studies among staphylococci and some other Gram-positive bacteria.
Volume: 10
Page(s): 357-362
Year: 1981
Ref #: 1372
Author(s): Ludwig,W.;Schleifer,K.H.;Fox,G.E.;Seewaldt,E.;Stackebrandt,E.
Journal: J. Gen. Microbiol.
Title: A phylogenetic analysis of staphylococci, Peptococcus saccharolyticus and Micrococcus mucilaginosus.
Volume: 125
Page(s): 357-366
Year: 1981
Ref #: 493
Author(s): Schleifer,K.H.;Kocur,M.
Journal: Arch. Mikrobiol.
Title: Classification of staphylococci based on chemical and biochemical properties.
Volume: 93
Page(s): 65-85
Year: 1973
Ref #: 499
Author(s): Silvestri,L.G.;Hill,L.R.
Journal: J. Bacteriol.
Title: Agreement between deoxyribonucleic acid base composition and taxometric classification of Gram-positive cocci.
Volume: 90
Page(s): 136-140
Year: 1965
Data: (ATCC 12600, CCM 885) Type strain / S. T. Cowan, NCTC Colindale in 1953 / Pleural fluid in 1935 / Cowans type III / Serotype 3 / Cowan, S. T. (1938) J. path. Bact. 46, 31; (1939) 48, 169 / Cowan, S. T. (1954) J. gen. Microbiol. 10, 174 / Opinion 17 (1958) Int. Bull. bact. Nomencl. Taxon. 8, 153
Accession Date: 01/01/1953
History: ISOLATED BY COWAN S T ,NCTC,COLINDALE LONDON,1935
Authority: Rosenbach 1884 (AL)
Depositor: COWAN S T
Taxonomy: TaxLink: S2775 (Staphylococcus aureus subsp. aureus Rosenbach 1884) - Date of change: 5/02/2003
Other: SerotypesReferences: Cowan, S. T. (1938) J. path. Bact. 46, 31; (1939) 48, 169, 545; Christie, R. & Keogh, E. V. (1940) J. path. Bact. 51, 189; Hobbs, B. C. (1948) J. Hyg., Camb. 46, 222
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