| Extended Bibliography: |
Show bibliography
| Ref #: |
43856 |
| Author(s): |
Naimi,A.;Beck,G.;Branlant,C. |
| Journal: |
Microbiology |
| Title: |
Primary and secondary structures of rRNA spacer regions in enterococci |
| Volume: |
143 ( Pt 3) |
| Page(s): |
823-34 |
| Year: |
1997 |
| Keyword(s): |
GENBANK/X87177
GENBANK/X87178
GENBANK/X87179
GENBANK/X87180
GENBANK/X87181
GENBANK/X87182
GENBANK/X87183
GENBANK/X87184
GENBANK/X87185
GENBANK/X87186
GENBANK/X87187
GENBANK/X87188
GENBANK/X87189
GENBANK/X87190
GENBANK/X87191
Base Sequence
Enterococcus/*genetics
Molecular Sequence Data
Nucleic Acid Conformation
RNA, Bacterial/chemistry/*genetics
RNA, Ribosomal/chemistry/*genetics
RNA, Transfer/genetics
Sequence Alignment
|
| Remarks: |
The 16S-23S and 23S-5S rRNA spacer DNA regions (spacer regions 1 and 2, respectively) from Enterococcus faecalis, Enterococcus faecium, Enterococcus hirae, Enterococcus durans and Enterococcus mundtii were amplified by PCR. Their nucleotide sequences were established and a secondary structure model showing the interaction between the two spacer regions was built. Whereas lactococci and Streptococcus sensu stricto are characterized by a single type of spacer region 1, the enterococci show a high degree of variability in this region; thus the spacer regions 1 with and without tRNA(Ala) were characterized. However, as shown for lactococci and Streptococcus sensu stricto, the tRNA(Ala) gene does not encode the 3'-terminal CCA trinucleotide. A putative antitermination signal is found downstream from the tRNA(Ala) gene. Based on comparison with Lactococcus lactis and Streptococcus thermophilus, a double-stranded processing stem is proposed. In E, hirae, one of the three different types of spacer region 1 contains no tRNA(Ala), but displays a 107 nt insertion that forms a long stem-loop structure. A similar insertion (115 nt in length) was found in E. faecium and base compensatory mutations preserve the ability to form the long stem-loop structure. Such insertions may correspond to mobile intervening sequences, as found in the 23S rRNA coding sequences of some Gram-negative bacteria. The spacer regions 1 and 2 from the three subgroups of streptococci were compared, and except for the tRNA(Ala) gene and the double-stranded processing sites, little similarity was found, which opens large possibilities for future development of DNA-based typing methods. |
| URL: |
9084166 |
|
| Ref #: |
43380 |
| Author(s): |
Tsiodras,S.;Gold,H.S.;Coakley,E.P.;Wennersten,C.;Moellering RC,J.r.;Eliopoulos,G.M. |
| Journal: |
J Clin Microbiol |
| Title: |
Diversity of domain V of 23S rRNA gene sequence in different Enterococcus species |
| Volume: |
38 |
| Page(s): |
3991-3 |
| Year: |
2000 |
| Keyword(s): |
Animals
Enterococcus/*genetics/isolation & purification
Genes, Bacterial
Genes, rRNA/*genetics
Gram-Positive Bacterial Infections/*microbiology
Humans
Molecular Sequence Data
Phylogeny
Polymerase Chain Reaction
RNA, Ribosomal, 23S/chemistry/*genetics
Sequence Analysis, DNA
*Variation (Genetics)
|
| Remarks: |
The highly conserved central loop of domain V of 23S RNA (nucleotides 2042 to 2628; Escherichia coli numbering) is implicated in peptidyltransferase activity and represents one of the target sites for macrolide, lincosamide, and streptogramin B antibiotics. DNA encoding domain V (590 bp) of several species of Enterococcus was amplified by PCR. Twenty enterococcal isolates were tested, including Enterococcus faecium (six isolates), Enterococcus faecalis, Enterococcus avium, Enterococcus durans, Enterococcus gallinarum, Enterococcus casseliflavus (two isolates of each), and Enterococcus raffinosus, Enterococcus mundtii, Enterococcus malodoratus, and Enterococcus hirae (one isolate of each). For all isolates, species identification by biochemical testing was corroborated by 16S rRNA gene sequencing. The sequence of domain V of the 23S rRNA gene from E. faecium and E. faecalis differed from those of all other enterococci. The domain V sequences of E. durans and E. hirae were identical. This was also true for E. gallinarum and E. casseliflavus. E. avium differed from E. casseliflavus by 23 bases, from E. durans by 16 bases, and from E. malodoratus by 2 bases. E. avium differed from E. raffinosus by one base. Despite the fact that domain V is considered to be highly conserved, substantial differences were identified between several enterococcal species. |
| URL: |
11060057 |
|
| Ref #: |
13719 |
| Author(s): |
Naimi,A.;Beck,G.;Branlant,C. |
| Journal: |
Microbiology |
| Title: |
Primary and secondary structures of rRNA spacer regions in enterococci |
| Volume: |
143 ( Pt 3) |
| Page(s): |
823-34 |
| Year: |
1997 |
| Keyword(s): |
GENBANK/X87177
GENBANK/X87178
GENBANK/X87179
GENBANK/X87180
GENBANK/X87181
GENBANK/X87182
GENBANK/X87183
GENBANK/X87184
GENBANK/X87185
GENBANK/X87186
GENBANK/X87187
GENBANK/X87188
GENBANK/X87189
GENBANK/X87190
GENBANK/X87191
Base Sequence
Enterococcus/*genetics
Molecular Sequence Data
Nucleic Acid Conformation
RNA, Bacterial/chemistry/*genetics
RNA, Ribosomal/chemistry/*genetics
RNA, Transfer/genetics
Sequence Alignment
Support, Non-U.S. Gov't
|
| Remarks: |
The 16S-23S and 23S-5S rRNA spacer DNA regions (spacer regions 1 and 2, respectively) from Enterococcus faecalis, Enterococcus faecium, Enterococcus hirae, Enterococcus durans and Enterococcus mundtii were amplified by PCR. Their nucleotide sequences were established and a secondary structure model showing the interaction between the two spacer regions was built. Whereas lactococci and Streptococcus sensu stricto are characterized by a single type of spacer region 1, the enterococci show a high degree of variability in this region; thus the spacer regions 1 with and without tRNA(Ala) were characterized. However, as shown for lactococci and Streptococcus sensu stricto, the tRNA(Ala) gene does not encode the 3'-terminal CCA trinucleotide. A putative antitermination signal is found downstream from the tRNA(Ala) gene. Based on comparison with Lactococcus lactis and Streptococcus thermophilus, a double-stranded processing stem is proposed. In E, hirae, one of the three different types of spacer region 1 contains no tRNA(Ala), but displays a 107 nt insertion that forms a long stem-loop structure. A similar insertion (115 nt in length) was found in E. faecium and base compensatory mutations preserve the ability to form the long stem-loop structure. Such insertions may correspond to mobile intervening sequences, as found in the 23S rRNA coding sequences of some Gram-negative bacteria. The spacer regions 1 and 2 from the three subgroups of streptococci were compared, and except for the tRNA(Ala) gene and the double-stranded processing sites, little similarity was found, which opens large possibilities for future development of DNA-based typing methods. |
| URL: |
97237711 |
|
| Ref #: |
13705 |
| Author(s): |
Tsiodras,S.;Gold,H.S.;Coakley,E.P.;Wennersten,C.;Moellering RC,J.r.;Eliopoulos,G.M. |
| Journal: |
J Clin Microbiol |
| Title: |
Diversity of domain V of 23S rRNA gene sequence in different Enterococcus species |
| Volume: |
38 |
| Page(s): |
3991-3 |
| Year: |
2000 |
| Keyword(s): |
Animal
Enterococcus/*genetics/isolation & purification
Genes, Bacterial
Genes, rRNA/*genetics
Gram-Positive Bacterial Infections/*microbiology
Human
Molecular Sequence Data
Phylogeny
Polymerase Chain Reaction
RNA, Ribosomal, 23S/chemistry/*genetics
Sequence Analysis, DNA
*Variation (Genetics)
|
| Remarks: |
The highly conserved central loop of domain V of 23S RNA (nucleotides 2042 to 2628; Escherichia coli numbering) is implicated in peptidyltransferase activity and represents one of the target sites for macrolide, lincosamide, and streptogramin B antibiotics. DNA encoding domain V (590 bp) of several species of Enterococcus was amplified by PCR. Twenty enterococcal isolates were tested, including Enterococcus faecium (six isolates), Enterococcus faecalis, Enterococcus avium, Enterococcus durans, Enterococcus gallinarum, Enterococcus casseliflavus (two isolates of each), and Enterococcus raffinosus, Enterococcus mundtii, Enterococcus malodoratus, and Enterococcus hirae (one isolate of each). For all isolates, species identification by biochemical testing was corroborated by 16S rRNA gene sequencing. The sequence of domain V of the 23S rRNA gene from E. faecium and E. faecalis differed from those of all other enterococci. The domain V sequences of E. durans and E. hirae were identical. This was also true for E. gallinarum and E. casseliflavus. E. avium differed from E. casseliflavus by 23 bases, from E. durans by 16 bases, and from E. malodoratus by 2 bases. E. avium differed from E. raffinosus by one base. Despite the fact that domain V is considered to be highly conserved, substantial differences were identified between several enterococcal species. |
| URL: |
20514229 |
|
| Ref #: |
2848 |
| Author(s): |
Collins,M.D.;Jones,D.;Farrow,J.A.E.;Kilpper-Bälz,R.;Schleifer,K.H. |
| Journal: |
Int. J. Syst. Bacteriol. |
| Title: |
Enterococcus avium nom. rev., comb. nov.; E. casseliflavus nom. rev., comb. nov.; E. durans nom. rev., comb. nov.; E. gallinarum comb. nov.; and E. malodoratus sp. nov. |
| Volume: |
34 |
| Page(s): |
220-223 |
| Year: |
1984 |
|
| Ref #: |
2851 |
| Author(s): |
Farrow,J.A.E.;Jones,D.;Phillips,B.A.;Collins,M.D. |
| Journal: |
J. Gen. Microbiol. |
| Title: |
Taxonomic studies on some group D streptococci. |
| Volume: |
129 |
| Page(s): |
1423-1432 |
| Year: |
1983 |
|
| Ref #: |
2852 |
| Author(s): |
Kilpper-Bälz,R.;Fischer,G.;Schleifer,K.H. |
| Journal: |
Curr. Microbiol. |
| Title: |
Nucleic acid hybridization of group N and group D streptococci. |
| Volume: |
7 |
| Page(s): |
245-250 |
| Year: |
1982 |
|
| Ref #: |
2853 |
| Author(s): |
Sherman,J.M.;Wing,H.U. |
| Journal: |
J. Dairy Sci. |
| Title: |
Streptococcus durans nov. sp. |
| Volume: |
20 |
| Page(s): |
165-167 |
| Year: |
1937 |
|
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