Culture Collections

Bacteria and Mycoplasmas detail

Conditions of Supply of Microbial Pathogens: Safety

Bacteria Collection: Streptococcus pyogenes

NCTC Number: NCTC 8198
Current Name: Streptococcus pyogenes
Original Strain Reference: SF 130
Other Collection No: ATCC 12344; DSM 20565
Previous Catalogue Name: Streptococcus pyogenes
Type Strain: Yes
Family: Streptococcaceae
Hazard Group (ACDP): 2
Release Restrictions: Terms & Conditions of Supply of Microbial Pathogens: Safety
Antigenic Properties: serovar group a
Conditions for growth on solid media: Columbia blood agar, 24-48 hours, 37°C, aerobic
Conditions for growth on liquid media: nutrient broth,37, facultative anaerobe
Isolated From: human, from a throat swab of scarlet fever case
Whole Genome Sequence:
Annotated Genome:
16S rRNA Gene Sequence: >gb|AB002521|ATCC 12344| NCIB 11841| MAFF 910217|Streptococcus pyogenes DNA for 16S rRNA, strain ATCC 12344, NCIB11841, MAFF 910217.| agagtttgatcctgg... >gb|AY347559|ATCC 12344|Streptococcus pyogenes strain ATCC 12344 16S-23S ribosomal RNAintergenic spacer, complete sequence.| aggaaatggaacacg...
23S rRNA Gene Sequence: >gb|AY347559|ATCC 12344|Streptococcus pyogenes strain ATCC 12344 16S-23S ribosomal RNAintergenic spacer, complete sequence.| aggaaatggaacacg... >gb|AB168125|ATCC 12344|Streptococcus pyogenes gene for 23S rRNA, complete sequence,strain:ATCC 12344.| ggttaagttaataag...
Bibliography: W.H.O. TECHN.REP.SER. 1959 172 24;ELLIOT S D 1943 BRIT.J.EXP.PATH. 24
Extended Bibliography: showhide Show bibliography
Ref #: 17178
Author(s): Kawata,K.;Anzai,T.;Senna,K.;Kikuchi,N.;Ezawa,A.;Takahashi,T.
Journal: FEMS Microbiol Lett
Title: Simple and rapid PCR method for identification of streptococcal species relevant to animal infections based on 23S rDNA sequence
Volume: 237
Page(s): 57-64
Year: 2004
Keyword(s): GENBANK/AB168118 GENBANK/AB168119 GENBANK/AB168120 GENBANK/AB168121 GENBANK/AB168122 GENBANK/AB168123 GENBANK/AB168124 GENBANK/AB168125 GENBANK/AB168126 GENBANK/AB168127 GENBANK/AB168128 Animals DNA, Bacterial/*analysis/chemistry DNA, Ribosomal/*analysis/chemistry Electrophoresis, Agar Gel Genes, rRNA Molecular Sequence Data Phylogeny Polymerase Chain Reaction/*methods RNA, Bacterial/genetics RNA, Ribosomal, 23S/genetics Sequence Analysis, DNA Streptococcal Infections/microbiology/*veterinary Streptococcus/*classification/genetics/*isolation & purification
Remarks: A PCR identification system targeting 23S rDNA sequences for the identification of eight streptococcal species relevant to animal infections (Streptococcus agalactiae, S. bovis, S. canis, S. dysgalactiae, S. equi, S. porcinus, S. suis and S. uberis) was developed. This system consists of two PCR reactions, A and B, in which seven and eight primers, respectively, are used simultaneously, and was designed so that each amplification product indicates a species by its size. A total of 111 cultures, including the type strain of eight species, could be successfully identified and differentiated as individual species, except for the cross reactivity between S. bovis and S. equinus. The developed PCR system can complete the identification procedure for eight streptococcal species through two tube reactions per isolate, and, therefore, might provide a rapid, simple and accurate diagnostic tool for veterinary laboratories.
URL: 15268938
Ref #: 82554
Author(s): Chen,C.C.;Teng,L.J.;Chang,T.C.
Journal: J Clin Microbiol
Title: Identification of clinically relevant viridans group streptococci by sequence analysis of the 16S-23S ribosomal DNA spacer region
Volume: 42
Page(s): 2651-7
Year: 2004
Keyword(s): DNA, Ribosomal Spacer/*chemistry Humans Phylogeny Polymerase Chain Reaction RNA, Ribosomal, 16S/*genetics RNA, Ribosomal, 23S/*genetics Sequence Analysis, DNA Viridans Streptococci/classification/genetics/*isolation & purification
Remarks: The feasibility of sequence analysis of the 16S-23S ribosomal DNA (rDNA) intergenic spacer (ITS) for the identification of clinically relevant viridans group streptococci (VS) was evaluated. The ITS regions of 29 reference strains (11 species) of VS were amplified by PCR and sequenced. These 11 species were Streptococcus anginosus, S. constellatus, S. gordonii, S. intermedius, S. mitis, S. mutans, S. oralis, S. parasanguinis, S. salivarius, S. sanguinis, and S. uberis. The ITS lengths (246 to 391 bp) and sequences were highly conserved among strains within a species. The intraspecies similarity scores for the ITS sequences ranged from 0.98 to 1.0, except for the score for S. gordonii strains. The interspecies similarity scores for the ITS sequences varied from 0.31 to 0.93. Phylogenetic analysis of the ITS regions revealed that evolution of the regions of some species of VS is not parallel to that of the 16S rRNA genes. One hundred six clinical isolates of VS were identified by the Rapid ID 32 STREP system (bioMerieux Vitek, Marcy l'Etoile, France) and by ITS sequencing, and the level of disagreement between the two methods was 18% (19 isolates). Most isolates producing discrepant results could be unambiguously assigned to a specific species by their ITS sequences. The accuracy of using ITS sequencing for identification of VS was verified by 16S rDNA sequencing for all strains except strains of S. oralis and S. mitis, which were difficult to differentiate by their 16S rDNA sequences. In conclusion, identification of species of VS by ITS sequencing is reliable and could be used as an alternative accurate method for identification of VS.
URL: 15184447
Ref #: 1300
Author(s): Skerman,V.B.D.;McGowan,V.;Sneath,P.H.A.(ed)
Journal: Int. J. Syst. Bacteriol.
Title: Approved Lists of Bacterial Names.
Volume: 30
Page(s): 225-420
Year: 1980
Ref #: 3344
Author(s): Kilpper-Bälz,R.;Schleifer,K.H.
Journal: FEMS Microbiol. Lett.
Title: Nucleic acid hybridization and cell wall composition studies of pyogenic streptococci.
Volume: 24
Page(s): 355-364
Year: 1984
Ref #: 6924
Author(s): DeutschesInstitutfürNormungDIN.NormenausschußMedizin(NAMed)
Title: DIN 58959-7. Qualitätsmanagement in der medizinischen Mikrobiologie. Teil 7: Allgemeine Anforderungen an das Mitführen von Kontrollstämmen. Beiblatt 2: ATCC- und DSM-Nummern häufig verwendeter Kontrollstämme.
Year: 1997
Data: (ATCC 12344, DSM 20565) Type strain / R. E. O. Williams, PHLS Colindale in 1950 / Scarlet fever / Preparation of group A antiserum / Vaccine strain for M-antiserum / Griffith, F. (1926) J. Hyg., Camb. 25, 385 / Hirst, G. K. & Lancefield, R. C. (1939) J. exp. Med. 69, 425 / Elliott, S. D. (1943) Br. J. exp. Path. 24, 159 / WHO Techn. Rep. Ser. (1959) 172, 24
Accession Date: 01/01/1950
Authority: Rosenbach 1884 (AL)
Depositor: WILLIAMS R E O
Taxonomy: TaxLink: S2860 (Streptococcus pyogenes Rosenbach 1884) - Date of change: 5/02/2003
Other: Serological Group A Griffith type 1
Biosafety Responsibility: It is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country

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The Culture Collections hold cell cultures, bacteria, fungi and virus strains from worldwide sources. Our scientists ensure that the identification of the cultures is correct and they remain unchanged from when they are first deposited with the Collection. Nevertheless, some of the data we provide about the cultures is supplied by the person depositing the strains and, although we have multiple checking procedures in place, we cannot always verify all their data. Please note that the Culture Collections cannot be held responsible for any inaccuracies in the data provided by the depositors.

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