| Extended Bibliography: |
Show bibliography
| Ref #: |
95493 |
| Author(s): |
Bootsma,H.J.;van der Heide,H.G.;van de Pas,S.;Schouls,L.M.;Mooi,F.R. |
| Journal: |
J Infect Dis |
| Title: |
Analysis of Moraxella catarrhalis by DNA typing: evidence for a distinct subpopulation associated with virulence traits |
| Volume: |
181 |
| Page(s): |
1376-87 |
| Year: |
2000 |
| Keyword(s): |
Adult
Antigens, Bacterial/genetics
Antigens, Surface/genetics
Bacterial Outer Membrane Proteins/genetics
Base Sequence
Carrier State
Child
Humans
Laryngitis/microbiology
Lung Diseases, Obstructive/genetics/microbiology
Molecular Sequence Data
Moraxella (Branhamella)
catarrhalis/classification/*genetics/*pathogenicity
Neisseriaceae Infections/genetics/microbiology
Polymorphism, Restriction Fragment Length
RNA, Ribosomal, 16S/chemistry
Respiratory Tract Infections/microbiology
|
| Remarks: |
Two DNA typing methods, probe-generated restriction fragment length polymorphism analysis and single-adapter amplified fragment length polymorphism analysis, were used to study the genetic relationships among 90 Moraxella catarrhalis strains. Both methods were found to be highly concordant, generating a dendrogram with 2 main branches. The division of the M. catarrhalis population into 2 subspecies was supported by analysis of the 16S rRNA sequences. Both beta-lactamase-positive and beta-lactamase-negative strains were found in all main branches, suggesting horizontal transfer of the beta-lactamase gene. In contrast, 2 virulence traits, complement resistance and adherence to epithelial cells, were strongly associated with 1 of the 2 subspecies. The branch depth suggested that complement-resistant adherent strains diverged from a common ancestor more recently than did complement-sensitive nonadherent strains. These findings suggest the existence of subpopulations of M. catarrhalis that differ in virulence, and they may have implications for vaccine development. |
| URL: |
10762569 |
|
| Ref #: |
48709 |
| Author(s): |
Dauga,C. |
| Journal: |
Int J Syst Evol Microbiol |
| Title: |
Evolution of the gyrB gene and the molecular phylogeny of Enterobacteriaceae: a model molecule for molecular systematic studies |
| Volume: |
52 |
| Page(s): |
531-47 |
| Year: |
2002 |
| Keyword(s): |
GENBANK/AJ300528
GENBANK/AJ300529
GENBANK/AJ300530
GENBANK/AJ300531
GENBANK/AJ300532
GENBANK/AJ300533
GENBANK/AJ300534
GENBANK/AJ300535
GENBANK/AJ300536
GENBANK/AJ300537
GENBANK/AJ300538
GENBANK/AJ300539
GENBANK/AJ300540
GENBANK/AJ300541
GENBANK/AJ300542
GENBANK/AJ300543
GENBANK/AJ300544
GENBANK/AJ300545
GENBANK/AJ300546
GENBANK/AJ300547
GENBANK/AJ300548
GENBANK/AJ300549
GENBANK/AJ300550
GENBANK/AJ300551
GENBANK/AJ300552
GENBANK/AJ300553
GENBANK/AJ300554
DNA Gyrase/*genetics
Enterobacteriaceae/*classification/genetics
Evolution, Molecular
Genes, rRNA
Molecular Sequence Data
Phenotype
RNA, Bacterial/chemistry
RNA, Ribosomal, 16S/chemistry
|
| Remarks: |
Phylogenetic trees showing the evolutionary relatedness of Enterobacteriaceae based upon gyrB and 16S rRNA genes were compared. Congruence among trees of these molecules indicates that the genomes of these species are not completely mosaic and that molecular systematic studies can be carried out. Phylogenetic trees based on gyrB sequences appeared to be more reliable at determining relationships among Serratia species than trees based on 16S rRNA gene sequences. gyrB sequences from Serratia species formed a monophyletic group validated by significant bootstrap values. Serratia fonticola had the most deeply branching gyrB sequence in the Serratia monophyletic group, which was consistent with its atypical phenotypic characteristics. Klebsiella and Enterobacter genera seemed to be polyphyletic, but the branching patterns of gyrB and 16S rRNA gene trees were not congruent. Enterobacter aerogenes was grouped with Klebsiella pneumoniae on the gyrB phylogenetic tree, which supports that this species could be transferred to the Klebsiella genus. Unfortunately, 16S rRNA and gyrB phylogenetic trees gave conflicting evolutionary relationships for Citrobacter freundii because of its unusual gyrB evolutionary process. gyrB lateral gene transfer was suspected for Hafnia alvei. Saturation of gyrB genes was observed by the pairwise comparison of Proteus spp., Providencia alcalifaciens and Morganella morganii sequences. Depending on their level of variability, 16S rRNA gene sequences were useful for describing phylogenetic relationships between distantly related Enterobacteriaceae, whereas gyrB sequence comparison was useful for inferring intra- and some intergeneric relationships. |
| URL: |
11931166 |
|
| Ref #: |
95457 |
| Author(s): |
Bodetti,T.J.;Jacobson,E.;Wan,C.;Hafner,L.;Pospischil,A.;Rose,K.;Timms,P. |
| Journal: |
Syst Appl Microbiol |
| Title: |
Molecular evidence to support the expansion of the hostrange of Chlamydophila pneumoniae to include reptiles as well as humans, horses, koalas and amphibians |
| Volume: |
25 |
| Page(s): |
146-52 |
| Year: |
2002 |
| Keyword(s): |
GENBANK/AY026515
GENBANK/AY026516
GENBANK/AY026517
GENBANK/AY026518
GENBANK/AY026519
GENBANK/AY026520
Amphibia/*microbiology
Animals
Bacterial Outer Membrane Proteins/chemistry
Chlamydophila Infections/diagnosis/microbiology
Chlamydophila pneumoniae/classification/*genetics/*isolation &
purification
Disease Reservoirs
Horses/microbiology
Humans
Immunohistochemistry
Marsupialia/microbiology
Molecular Sequence Data
Polymerase Chain Reaction/methods
RNA, Bacterial/analysis
RNA, Ribosomal, 16S/analysis
Reptiles/classification/*microbiology
Sensitivity and Specificity
Sequence Analysis, DNA
Species Specificity
|
| Remarks: |
The Chlamydiales are a family of unique intracellular pathogens that cause significant disease in humans, birds and a wide range of animal hosts. Of the currently recognized species, Chlamydophila (previously Chlamydia) pneumoniae, unlike the other chlamydial species, has been previously considered to be solely a pathogen of humans, causing significant respiratory disease and has also been strongly connected with cardiovascular disease. Here we report the finding that strains of C. pneumoniae are widespread in the environment, being detected by molecular methods in a range of reptiles (snakes, iguanas, chameleons) and amphibians (frogs, turtles). Of particular interest was the finding that genotyping of the chlamydial major outer membrane protein gene in these newly identified C. pneumoniae strains showed that many were genetically very similar, if not identical to the human respiratory strains. Whether these reptilian and amphibian strains of C. pneumoniae are still capable of infecting humans, or crossed the host barrier some time ago, remains to be determined but may provide further insights into the relationship of this common respiratory infection with its human host. |
| URL: |
12086181 |
|
| Ref #: |
13696 |
| Author(s): |
Bootsma,H.J.;van der Heide,H.G.;van de Pas,S.;Schouls,L.M.;Mooi,F.R. |
| Journal: |
J Infect Dis |
| Title: |
Analysis of Moraxella catarrhalis by DNA typing: evidence for a distinct subpopulation associated with virulence traits |
| Volume: |
181 |
| Page(s): |
1376-87 |
| Year: |
2000 |
| Keyword(s): |
Adult
Antigens, Bacterial/genetics
Antigens, Surface/genetics
Bacterial Outer Membrane Proteins/genetics
Base Sequence
Carrier State
Child
Human
Laryngitis/microbiology
Lung Diseases, Obstructive/genetics/microbiology
Molecular Sequence Data
Moraxella (Branhamella)
catarrhalis/classification/*genetics/*pathogenicity
Neisseriaceae Infections/genetics/microbiology
Polymorphism, Restriction Fragment Length
RNA, Ribosomal, 16S/chemistry
Respiratory Tract Infections/microbiology
Support, Non-U.S. Gov't
|
| Remarks: |
Two DNA typing methods, probe-generated restriction fragment length polymorphism analysis and single-adapter amplified fragment length polymorphism analysis, were used to study the genetic relationships among 90 Moraxella catarrhalis strains. Both methods were found to be highly concordant, generating a dendrogram with 2 main branches. The division of the M. catarrhalis population into 2 subspecies was supported by analysis of the 16S rRNA sequences. Both beta-lactamase-positive and beta-lactamase-negative strains were found in all main branches, suggesting horizontal transfer of the beta-lactamase gene. In contrast, 2 virulence traits, complement resistance and adherence to epithelial cells, were strongly associated with 1 of the 2 subspecies. The branch depth suggested that complement-resistant adherent strains diverged from a common ancestor more recently than did complement-sensitive nonadherent strains. These findings suggest the existence of subpopulations of M. catarrhalis that differ in virulence, and they may have implications for vaccine development. |
| URL: |
20227761 |
|
|
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