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Bacteria Collection: Moraxella lacunata

NCTC Number: NCTC 7911
Current Name: Moraxella lacunata
Original Strain Reference: ATCC 17952
Other Collection No: ATCC 17952; CCUG 4862; LMG 1009; NCDC KC 749
Previous Catalogue Name: Moraxella liquefaciens
Type Strain: No
Family: Moraxellaceae
Hazard Group (ACDP): 2
Release Restrictions: Terms & Conditions of Supply of Microbial Pathogens: Safety
Conditions for growth on solid media: Columbia blood agar, 24-48 hours, 37°C, aerobic
Conditions for growth on liquid media: nutrient broth,37, aerobic
Isolated From: human, childs eye with conjunctivitis
Whole Genome Sequence: http://www.ebi.ac.uk/ena/data/view/ERS1211132
16S rRNA Gene Sequence: >gb|AF005169|NCTC 7911|Moraxella lacunata 16S ribosomal RNA gene, partial sequence.| ctggcggcaggctta...
Extended Bibliography: showhide Show bibliography
Ref #: 58671
Author(s): Pettersson,B.;Kodjo,A.;Ronaghi,M.;Uhlen,M.;Tonjum,T.
Journal: Int J Syst Bacteriol
Title: Phylogeny of the family Moraxellaceae by 16S rDNA sequence analysis, with special emphasis on differentiation of Moraxella species
Volume: 48 Pt 1
Page(s): 75-89
Year: 1998
Keyword(s): GENBANK/AF005160 GENBANK/AF005161 GENBANK/AF005162 GENBANK/AF005163 GENBANK/AF005164 GENBANK/AF005165 GENBANK/AF005166 GENBANK/AF005167 GENBANK/AF005168 GENBANK/AF005169 GENBANK/AF005170 GENBANK/AF005171 GENBANK/AF005172 GENBANK/AF005173 GENBANK/AF005174 GENBANK/AF005175 GENBANK/AF005176 GENBANK/AF005177 GENBANK/AF005178 GENBANK/AF005179 GENBANK/AF005180 GENBANK/AF005181 GENBANK/AF005182 GENBANK/AF005183 GENBANK/AF005184 GENBANK/AF005185 GENBANK/AF005186 GENBANK/AF005187 GENBANK/AF005188 GENBANK/AF005189 etc. DNA Primers DNA, Bacterial/analysis Molecular Sequence Data Moraxella (Branhamella) catarrhalis/*chemistry/*genetics Moraxella (Moraxella) bovis/*classification/*genetics Oligonucleotides Phylogeny RNA, Bacterial/analysis RNA, Ribosomal, 16S/*analysis Species Specificity
Remarks: Thirty-three strains previously classified into 11 species in the bacterial family Moraxellaceae were subjected to phylogenetic analysis based on 16S rRNA sequences. The family Moraxellaceae formed a distinct clade consisting of four phylogenetic groups as judged from branch lengths, bootstrap values and signature nucleotides. Group I contained the classical moraxellae and strains of the coccal moraxellae, previously known as Branhamella, with 16S rRNA similarity of > or = 95%. A further division of group I into five tentative clusters is discussed. Group II consisted of two strains representing Moraxella atlantae and Moraxella osloensis. These strains were only distantly related to each other (93.4%) and also to the other members of the Moraxellaceae (< or = 93%). Therefore, reasons for reclassification of these species into separate and new genera are discussed. Group III harboured strains of the genus Psychrobacter and strain 752/52 of [Moraxella] phenylpyruvica. This strain of [M.] phenylpyruvica formed an early branch from the group III line of descent. Interestingly, a distant relationship was found between Psychrobacter phenylpyruvicus strain ATCC 23333T (formerly classified as [M.] phenylpyruvica) and [M.] phenylpyruvica strain 752/52, exhibiting less than 96% nucleotide similarity between their 16S rRNA sequences. The establishment of a new genus for [M.] phenylpyruvica strain 752/52 is therefore suggested. Group IV contained only two strains of the genus Acinetobacter. Strategies for the development of diagnostic probes and distinctive sequences for 16S rRNA-based species-specific assays within group I are suggested. Although these findings add to the classificatory placements within the Moraxellaceae, analysis of a more comprehensive selection of strains is still needed to obtain a complete classification system within this family.
URL: 9542079
Ref #: 13137
Author(s): Pettersson,B.;Kodjo,A.;Ronaghi,M.;Uhlen,M.;Tonjum,T.
Journal: Int J Syst Bacteriol
Title: Phylogeny of the family Moraxellaceae by 16S rDNA sequence analysis, with special emphasis on differentiation of Moraxella species
Volume: 48 Pt 1
Page(s): 75-89
Year: 1998
Keyword(s): GENBANK/AF005160 GENBANK/AF005161 GENBANK/AF005162 GENBANK/AF005163 GENBANK/AF005164 GENBANK/AF005165 GENBANK/AF005166 GENBANK/AF005167 GENBANK/AF005168 GENBANK/AF005169 GENBANK/AF005170 GENBANK/AF005171 GENBANK/AF005172 GENBANK/AF005173 GENBANK/AF005174 GENBANK/AF005175 GENBANK/AF005176 GENBANK/AF005177 GENBANK/AF005178 GENBANK/AF005179 GENBANK/AF005180 GENBANK/AF005181 GENBANK/AF005182 GENBANK/AF005183 GENBANK/AF005184 GENBANK/AF005185 GENBANK/AF005186 GENBANK/AF005187 GENBANK/AF005188 GENBANK/AF005189 etc. DNA Primers DNA, Bacterial/analysis Molecular Sequence Data Moraxella (Branhamella) catarrhalis/*chemistry/*genetics Moraxella (Moraxella) bovis/*classification/*genetics Oligonucleotides Phylogeny RNA, Bacterial/analysis RNA, Ribosomal, 16S/*analysis Species Specificity Support, Non-U.S. Gov't
Remarks: Thirty-three strains previously classified into 11 species in the bacterial family Moraxellaceae were subjected to phylogenetic analysis based on 16S rRNA sequences. The family Moraxellaceae formed a distinct clade consisting of four phylogenetic groups as judged from branch lengths, bootstrap values and signature nucleotides. Group I contained the classical moraxellae and strains of the coccal moraxellae, previously known as Branhamella, with 16S rRNA similarity of > or = 95%. A further division of group I into five tentative clusters is discussed. Group II consisted of two strains representing Moraxella atlantae and Moraxella osloensis. These strains were only distantly related to each other (93.4%) and also to the other members of the Moraxellaceae (< or = 93%). Therefore, reasons for reclassification of these species into separate and new genera are discussed. Group III harboured strains of the genus Psychrobacter and strain 752/52 of [Moraxella] phenylpyruvica. This strain of [M.] phenylpyruvica formed an early branch from the group III line of descent. Interestingly, a distant relationship was found between Psychrobacter phenylpyruvicus strain ATCC 23333T (formerly classified as [M.] phenylpyruvica) and [M.] phenylpyruvica strain 752/52, exhibiting less than 96% nucleotide similarity between their 16S rRNA sequences. The establishment of a new genus for [M.] phenylpyruvica strain 752/52 is therefore suggested. Group IV contained only two strains of the genus Acinetobacter. Strategies for the development of diagnostic probes and distinctive sequences for 16S rRNA-based species-specific assays within group I are suggested. Although these findings add to the classificatory placements within the Moraxellaceae, analysis of a more comprehensive selection of strains is still needed to obtain a complete classification system within this family.
URL: 98203182
Data: B. Wheatley, Leeds in 1949 / Bacterium duplex var. liquefaciens
Accession Date: 01/01/1949
Depositor: WHEATLEY B
Taxonomy: TaxLink: ? (NCTC 7911) - Date of change: 04/02/2003
Biosafety Responsibility: It is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country

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