| Extended Bibliography: |
Show bibliography
| Ref #: |
95527 |
| Author(s): |
Kawamura,Y.;Hou,X.G.;Sultana,F.;Miura,H.;Ezaki,T. |
| Journal: |
Int J Syst Bacteriol |
| Title: |
Determination of 16S rRNA sequences of Streptococcus mitis and Streptococcus gordonii and phylogenetic relationships among members of the genus Streptococcus |
| Volume: |
45 |
| Page(s): |
406-8 |
| Year: |
1995 |
| Keyword(s): |
GENBANK/D38482
GENBANK/D38483
DNA, Bacterial/*genetics
Molecular Sequence Data
Phylogeny
RNA, Bacterial/*genetics
RNA, Ribosomal, 16S/*genetics
Streptococcus/*classification/genetics
|
| Remarks: |
We determined the 16S rRNA sequences of the type strains of Streptococcus mitis and Streptococcus gordonii and calculated the phylogenetic distances between those organisms and other members of the genus Streptococcus. The viridans group streptococci were separated into five phylogenetic groups; we named these groups the anginosus group, the mitis group, the salivarius group, the bovis group, and the mutans group. S. mitis and S. gordonii clustered in the mitis group together with Streptococcus pneumoniae, Streptococcus oralis, Streptococcus sanguis, and Streptococcus parasanguis at levels of sequence homology of more than 96%. Within this group, S. mitis, S. oralis, and S. pneumoniae exhibited more than 99% sequence homology with each other, although the DNA-DNA similarity values for their total chromosome DNAs were less than 60%. |
| URL: |
7537076 |
|
| Ref #: |
82554 |
| Author(s): |
Chen,C.C.;Teng,L.J.;Chang,T.C. |
| Journal: |
J Clin Microbiol |
| Title: |
Identification of clinically relevant viridans group streptococci by sequence analysis of the 16S-23S ribosomal DNA spacer region |
| Volume: |
42 |
| Page(s): |
2651-7 |
| Year: |
2004 |
| Keyword(s): |
DNA, Ribosomal Spacer/*chemistry
Humans
Phylogeny
Polymerase Chain Reaction
RNA, Ribosomal, 16S/*genetics
RNA, Ribosomal, 23S/*genetics
Sequence Analysis, DNA
Viridans Streptococci/classification/genetics/*isolation & purification
|
| Remarks: |
The feasibility of sequence analysis of the 16S-23S ribosomal DNA (rDNA) intergenic spacer (ITS) for the identification of clinically relevant viridans group streptococci (VS) was evaluated. The ITS regions of 29 reference strains (11 species) of VS were amplified by PCR and sequenced. These 11 species were Streptococcus anginosus, S. constellatus, S. gordonii, S. intermedius, S. mitis, S. mutans, S. oralis, S. parasanguinis, S. salivarius, S. sanguinis, and S. uberis. The ITS lengths (246 to 391 bp) and sequences were highly conserved among strains within a species. The intraspecies similarity scores for the ITS sequences ranged from 0.98 to 1.0, except for the score for S. gordonii strains. The interspecies similarity scores for the ITS sequences varied from 0.31 to 0.93. Phylogenetic analysis of the ITS regions revealed that evolution of the regions of some species of VS is not parallel to that of the 16S rRNA genes. One hundred six clinical isolates of VS were identified by the Rapid ID 32 STREP system (bioMerieux Vitek, Marcy l'Etoile, France) and by ITS sequencing, and the level of disagreement between the two methods was 18% (19 isolates). Most isolates producing discrepant results could be unambiguously assigned to a specific species by their ITS sequences. The accuracy of using ITS sequencing for identification of VS was verified by 16S rDNA sequencing for all strains except strains of S. oralis and S. mitis, which were difficult to differentiate by their 16S rDNA sequences. In conclusion, identification of species of VS by ITS sequencing is reliable and could be used as an alternative accurate method for identification of VS. |
| URL: |
15184447 |
|
| Ref #: |
82526 |
| Author(s): |
Hoshino,T.;Fujiwara,T.;Kilian,M. |
| Journal: |
J Clin Microbiol |
| Title: |
Use of phylogenetic and phenotypic analyses to identify nonhemolytic streptococci isolated from bacteremic patients |
| Volume: |
43 |
| Page(s): |
6073-85 |
| Year: |
2005 |
| Keyword(s): |
GENBANK/AB199330
GENBANK/AB199331
GENBANK/AB199332
GENBANK/AB199333
GENBANK/AB199334
GENBANK/AB199335
GENBANK/AB199336
GENBANK/AB199337
GENBANK/AB199338
GENBANK/AB199339
GENBANK/AB199340
GENBANK/AB199341
GENBANK/AB199342
GENBANK/AB199343
GENBANK/AB199344
GENBANK/AB199345
GENBANK/AB199346
GENBANK/AB199347
GENBANK/AB199348
GENBANK/AB199349
GENBANK/AB199350
GENBANK/AB199351
GENBANK/AB199352
GENBANK/AB199353
GENBANK/AB199354
GENBANK/AB199355
GENBANK/AB199356
GENBANK/AB199357
GENBANK/AB199358
GENBANK/AB199359
GENBANK/AB199360
GENBANK/AB199361
GENBANK/AB199362
GENBANK/AB199363
GENBANK/AB199364
GENBANK/AB199365
GENBANK/AB199366
GENBANK/AB199367
GENBANK/AB199368
GENBANK/AB199369
GENBANK/AB199370
GENBANK/AB199371
GENBANK/AB199372
GENBANK/AB199373
GENBANK/AB199374
GENBANK/AB199375
GENBANK/AB199376
GENBANK/AB199377
GENBANK/AB199378
GENBANK/AB199379
GENBANK/AB199380
GENBANK/AB199381
GENBANK/AB199382
GENBANK/AB199383
GENBANK/AB199384
GENBANK/AB199385
GENBANK/AB199386
GENBANK/AB199387
GENBANK/AB199388
GENBANK/AB199389
GENBANK/AB199390
GENBANK/AB199391
GENBANK/AB199392
GENBANK/AB199393
GENBANK/AB199394
GENBANK/AB199395
GENBANK/AB199396
GENBANK/AB199397
GENBANK/AB199398
GENBANK/AB199399
GENBANK/AB199400
GENBANK/AB199401
GENBANK/AB199402
GENBANK/AB199403
GENBANK/AB199404
GENBANK/AB199405
GENBANK/AB199406
GENBANK/AB199407
GENBANK/AB199408
GENBANK/AB199409
GENBANK/AB199410
GENBANK/AB199411
GENBANK/AB199412
GENBANK/AB199413
GENBANK/AB199414
GENBANK/AB199415
GENBANK/AB199416
GENBANK/AB199417
GENBANK/AB199418
GENBANK/AB199419
GENBANK/AB199420
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GENBANK/AB199422
GENBANK/AB199423
GENBANK/AB199424
GENBANK/AB199425
GENBANK/AB199426
GENBANK/AB199427
GENBANK/AB199428
GENBANK/AB199429
GENBANK/AB199430
GENBANK/AB199431
GENBANK/AB199432
GENBANK/AB199433
GENBANK/AB199434
GENBANK/AB199435
GENBANK/AB199436
GENBANK/AB199437
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GENBANK/AB199441
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GENBANK/AB199450
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GENBANK/AB199459
GENBANK/AB199460
GENBANK/AB199461
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GENBANK/AB199464
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GENBANK/AB199477
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GENBANK/AB199480
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GENBANK/AB199482
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GENBANK/AB199490
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GENBANK/AB200106
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GENBANK/AB200111
GENBANK/AB200112
GENBANK/AB200113
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GENBANK/AB200115
GENBANK/AB200116
GENBANK/AB200117
GENBANK/AB200118
GENBANK/AB200119
GENBANK/AB200120
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GENBANK/AB200125
GENBANK/AB200126
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GENBANK/AB200128
GENBANK/AB200129
GENBANK/AB200130
GENBANK/AB200131
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GENBANK/AB200133
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GENBANK/AB200136
GENBANK/AB200137
GENBANK/AB200138
GENBANK/AB200139
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GENBANK/AB200141
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GENBANK/AB200143
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GENBANK/AB200145
GENBANK/AB200146
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GENBANK/AB200148
GENBANK/AB200149
GENBANK/AB200150
GENBANK/AB200151
GENBANK/AB200152
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GENBANK/AB200155
GENBANK/AB200156
GENBANK/AB200157
GENBANK/AB200158
GENBANK/AB200159
GENBANK/AB200160
GENBANK/AB200161
GENBANK/AB200162
GENBANK/AB200163
GENBANK/AB200164
GENBANK/AB200165
GENBANK/AB200166
GENBANK/AB200167
GENBANK/AB218984
GENBANK/AB218985
Bacteremia/*microbiology
Bacterial Proteins/genetics
*Bacterial Typing Techniques
*Hemolysis
Humans
Molecular Sequence Data
Phenotype
*Phylogeny
Polymerase Chain Reaction/methods
Reagent Kits, Diagnostic
Sequence Analysis, DNA
Species Specificity
Streptococcal Infections/microbiology
Streptococcus/*classification/genetics/isolation &
purification/*physiology
|
| Remarks: |
The aim of this study was to evaluate molecular and phenotypic methods for the identification of nonhemolytic streptococci. A collection of 148 strains consisting of 115 clinical isolates from cases of infective endocarditis, septicemia, and meningitis and 33 reference strains, including type strains of all relevant Streptococcus species, were examined. Identification was performed by phylogenetic analysis of nucleotide sequences of four housekeeping genes, ddl, gdh, rpoB, and sodA; by PCR analysis of the glucosyltransferase (gtf) gene; and by conventional phenotypic characterization and identification using two commercial kits, Rapid ID 32 STREP and STREPTOGRAM and the associated databases. A phylogenetic tree based on concatenated sequences of the four housekeeping genes allowed unequivocal differentiation of recognized species and was used as the reference. Analysis of single gene sequences revealed deviation clustering in eight strains (5.4%) due to homologous recombination with other species. This was particularly evident in S. sanguinis and in members of the anginosus group of streptococci. The rate of correct identification of the strains by both commercial identification kits was below 50% but varied significantly between species. The most significant problems were observed with S. mitis and S. oralis and 11 Streptococcus species described since 1991. Our data indicate that identification based on multilocus sequence analysis is optimal. As a more practical alternative we recommend identification based on sodA sequences with reference to a comprehensive set of sequences that is available for downloading from our server. An analysis of the species distribution of 107 nonhemolytic streptococci from bacteremic patients showed a predominance of S. oralis and S. anginosus with various underlying infections. |
| URL: |
16333101 |
|
| Ref #: |
17297 |
| Author(s): |
Glazunova,O.O.;Raoult,D.;Roux,V. |
| Journal: |
Int J Syst Evol Microbiol |
| Title: |
Streptococcus massiliensis sp. nov., isolated from a patient blood culture |
| Volume: |
56 |
| Page(s): |
1127-31 |
| Year: |
2006 |
| Keyword(s): |
GENBANK/AY769997
GENBANK/AY769998
GENBANK/AY769999
GENBANK/AY770000
GENBANK/AY770001
GENBANK/AY770002
GENBANK/DQ132983
GENBANK/DQ132984
GENBANK/DQ132985
GENBANK/DQ132986
GENBANK/DQ132987
GENBANK/DQ232458
GENBANK/DQ232560
Bacterial Proteins/genetics
Bacterial Typing Techniques
Blood/*microbiology
DNA, Bacterial/chemistry/genetics
DNA, Ribosomal/chemistry/genetics
DNA-Directed RNA Polymerases/genetics
Genes, rRNA
Humans
Male
Middle Aged
Molecular Sequence Data
Phylogeny
RNA, Bacterial/genetics
RNA, Ribosomal, 16S/genetics
Streptococcal Infections/*microbiology
Superoxide Dismutase/genetics
Viridans Streptococci/*classification/genetics/*isolation &
purification/physiology
|
| Remarks: |
An unidentified strain of the viridans group of streptococci was isolated from a human blood sample. It was distinguished from all other recognized species of the Streptococcus sanguinis group by several biochemical characteristics. Phylogenetic analysis based on 16S rRNA gene sequence comparisons clustered this strain with Streptococcus ferus (mutans group) but phylogenetic analysis based on rpoB and sodA gene sequence comparisons included it in the S. sanguinis group. The isolate showed 95.4 and 95.2 % 16S rRNA gene sequence similarity to S. ferus and S. sanguinis, respectively, confirming it as belonging to a novel taxon, for which the name Streptococcus massiliensis sp. nov. is proposed. The type strain is 4401825T (=CIP 108498T=CCUG 49690T). |
| URL: |
16627666 |
|
| Ref #: |
13406 |
| Author(s): |
Kawamura,Y.;Hou,X.G.;Sultana,F.;Miura,H.;Ezaki,T. |
| Journal: |
Int J Syst Bacteriol |
| Title: |
Determination of 16S rRNA sequences of Streptococcus mitis and Streptococcus gordonii and phylogenetic relationships among members of the genus Streptococcus |
| Volume: |
45 |
| Page(s): |
406-8 |
| Year: |
1995 |
| Keyword(s): |
GENBANK/D38482
GENBANK/D38483
DNA, Bacterial/*genetics
Molecular Sequence Data
Phylogeny
RNA, Bacterial/*genetics
RNA, Ribosomal, 16S/*genetics
Streptococcus/*classification/genetics
|
| Remarks: |
We determined the 16S rRNA sequences of the type strains of Streptococcus mitis and Streptococcus gordonii and calculated the phylogenetic distances between those organisms and other members of the genus Streptococcus. The viridans group streptococci were separated into five phylogenetic groups; we named these groups the anginosus group, the mitis group, the salivarius group, the bovis group, and the mutans group. S. mitis and S. gordonii clustered in the mitis group together with Streptococcus pneumoniae, Streptococcus oralis, Streptococcus sanguis, and Streptococcus parasanguis at levels of sequence homology of more than 96%. Within this group, S. mitis, S. oralis, and S. pneumoniae exhibited more than 99% sequence homology with each other, although the DNA-DNA similarity values for their total chromosome DNAs were less than 60%. |
| URL: |
95244327 |
|
| Ref #: |
3965 |
| Author(s): |
Kilian,M.;Mikkelsen,L.;Henrichsen,J. |
| Journal: |
Int. J. Syst. Bacteriol. |
| Title: |
Taxonomic study of viridans streptococci: Description of Streptococcus gordonii sp. nov. and emended descriptions of Streptococcus sanguis (White and Niven 1946), Streptococcus oralis (Bridge and Sneath 1982), and Streptococcus mitis (Andrewes and Horder |
| Volume: |
39 |
| Page(s): |
471-484 |
| Year: |
1989 |
|
| Ref #: |
3969 |
| Author(s): |
Schmidhuber,S.;Kilpper-Bälz,R.;Schleifer,K.H. |
| Journal: |
System. Appl. Microbiol. |
| Title: |
A taxonomic study of Streptococcus mitis, S. oralis, and S. sanguis. |
| Volume: |
10 |
| Page(s): |
74-77 |
| Year: |
1987 |
|
|
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