Culture Collections

Bacteria and Mycoplasmas detail

Conditions of Supply of Microbial Pathogens: Safety





Bacteria Collection: Haemophilus parainfluenzae

NCTC Number: NCTC 7857
Current Name: Haemophilus parainfluenzae
Original Strain Reference: Wound
Other Collection No: ATCC 33392; DSM 8978; WOUND
Previous Catalogue Name: Haemophilus parainfluenzae
Type Strain: Yes
Family: Pasteurellaceae
Hazard Group (ACDP): 2
Release Restrictions: Terms & Conditions of Supply of Microbial Pathogens: Safety
Conditions for growth on solid media: Chocolate blood agar, 24-48 hours, 37°C, requires carbon dioxide
Conditions for growth on liquid media: nutrient broth,37, facultative anaerobe
Isolated From: human, from septic finger
Whole Genome Sequence: http://www.ebi.ac.uk/ena/data/view/ERS1509359
Annotated Genome: ftp://ftp.sanger.ac.uk/pub/project/pathogens/NCTC3000/...
Miscellaneous Sequence Data: >gb|AJ617792|DSM 8978|Haemophilus parainfluenzae partial gyrB gene for DNA gyrase subunitB.| ggataccgatgatgg...
Extended Bibliography: showhide Show bibliography
Ref #: 39176
Author(s): Roth,S.B.;Jalava,J.;Ruuskanen,O.;Ruohola,A.;Nikkari,S.
Journal: J Clin Microbiol
Title: Use of an oligonucleotide array for laboratory diagnosis of bacteria responsible for acute upper respiratory infections
Volume: 42
Page(s): 4268-74
Year: 2004
Keyword(s): Bacterial Infections/*diagnosis Base Sequence DNA Primers DNA Topoisomerases, Type I/genetics Humans Oligonucleotide Array Sequence Analysis/*methods Oligonucleotide Probes Polymerase Chain Reaction Respiratory Tract Infections/*diagnosis Sensitivity and Specificity
Remarks: We developed a diagnostic array of oligonucleotide probes targeting species-specific variable regions of the genes encoding topoisomerases GyrB and ParE of respiratory bacterial pathogens. Suitable broad-range primer sequences were designed based on alignment of gyrB/parE sequences from nine different bacterial species. These species included Corynebacterium diphtheriae, Fusobacterium necrophorum, Haemophilus influenzae, Legionella pneumophila, Moraxella catarrhalis, Mycoplasma pneumoniae, Staphylococcus aureus, Streptococcus pneumoniae, and Streptococcus pyogenes. Specific probe sequences were selected by comparative analysis against the European Bioinformatics Database, as well as gyrB/parE sequences generated for this study. To verify specificity, at least six initial oligonucleotide probe sequences per bacterial species were tested by hybridization on a solid glass support using culture collection strains as templates. Finally, three oligonucleotide probes per bacterial species were utilized to examine 65 middle ear fluid and 29 throat swab samples. The sensitivities of the developed assay compared to classic culture from middle ear fluid samples for H. influenzae, M. catarrhalis, and S. pneumoniae were 96 (93 for culture), 73 (93 for culture), and 100% (78% for culture), respectively. No cross-reactivity with bacterial species belonging to the normal oral flora was observed when the 29 throat swab samples were studied. The sensitivity of the assay to detect S. pyogenes from these samples was 93% (80% for culture). These results provide a proof of concept for the diagnostic use of microarray technology based on broad-range topoisomerase gene amplification, followed by hybridization and specific detection of bacterial species.
URL: 15365022
Ref #: 1300
Author(s): Skerman,V.B.D.;McGowan,V.;Sneath,P.H.A.(ed)
Journal: Int. J. Syst. Bacteriol.
Title: Approved Lists of Bacterial Names.
Volume: 30
Page(s): 225-420
Year: 1980
Ref #: 5070
Author(s): Kilian,M.
Journal: J. Gen. Microbiol.
Title: A taxonomic study of the genus Haemophilus, with the proposal of a new species.
Volume: 93
Page(s): 9-62
Year: 1976
Ref #: 7016
Author(s): Busse,H.-J.;Bunka,S.;Hensel,A.;Lubitz,W.
Journal: Int. J. Syst. Bacteriol.
Title: Discrimination of members of the family Pasteurellaceae based on polyamine patterns.
Volume: 47
Page(s): 698-708
Year: 1997
Data: Type strain / A. B. Rosher, London in 1949 / Septic finger
Accession Date: 01/01/1949
History: ISOLATED BY WHITE G
Authority: Rivers 1922 (AL)
Depositor: ROSHER A B
Taxonomy: TaxLink: S1397 (Haemophilus parainfluenzae Rivers 1922) - Date of change: 5/02/2003
Biosafety Responsibility: It is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country

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The Culture Collections hold cell cultures, bacteria, fungi and virus strains from worldwide sources. Our scientists ensure that the identification of the cultures is correct and they remain unchanged from when they are first deposited with the Collection. Nevertheless, some of the data we provide about the cultures is supplied by the person depositing the strains and, although we have multiple checking procedures in place, we cannot always verify all their data. Please note that the Culture Collections cannot be held responsible for any inaccuracies in the data provided by the depositors.

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