Culture Collections

Bacteria and Mycoplasmas detail

Conditions of Supply of Microbial Pathogens: Safety

Bacteria Collection: Staphylococcus saprophyticus subsp. saprophyticus

NCTC Number: NCTC 7692
Current Name: Staphylococcus saprophyticus subsp. saprophyticus
Original Strain Reference: Parker IC-131
Other Collection No: ATCC 15305; PARKER IC-131
Previous Catalogue Name: Staphylococcus saprophyticus
Type Strain: No
Family: Staphylococcaceae
Hazard Group (ACDP): 2
Release Restrictions: Terms & Conditions of Supply of Microbial Pathogens: Safety
Conditions for growth on solid media: Nutrient agar, 24-48 hours, 37°C, aerobic
Conditions for growth on liquid media: nutrient broth,37, facultative anaerobe
Isolated From: milk;ice cream after salt medium extraction
Whole Genome Sequence:
Annotated Genome:
16S rRNA Gene Sequence: >gb|D83371|MAFF911473| ATCC15305T|Staphylococcus saprophyticus (strain MAFF911473, ATCC15305T) genefor 16S rRNA, partial sequence.| aggatgaacgctggc...
Extended Bibliography: showhide Show bibliography
Ref #: 80948
Author(s): Drancourt,M.;Raoult,D.
Journal: J Clin Microbiol
Title: rpoB gene sequence-based identification of Staphylococcus species
Volume: 40
Page(s): 1333-8
Year: 2002
Keyword(s): *Bacterial Typing Techniques DNA-Directed RNA Polymerases/*genetics Databases, Factual Molecular Sequence Data Phylogeny Polymerase Chain Reaction *Sequence Analysis, DNA Staphylococcus/*classification/genetics
Remarks: The complete sequence of rpoB, the gene encoding the beta subunit of RNA polymerase was determined for Staphylococcus saccharolyticus, Staphylococcus lugdunensis, S taphylococcus caprae, and Staphylococcus intermedius and partial sequences were obtained for an additional 27 Staphylococcus species. The complete rpoB sequences varied in length from 3,452 to 3,845 bp and had a 36.8 to 39.2% GC content. The partial sequences had 71.6 to 93.6% interspecies homology and exhibited a 0.08 to 0.8% intraspecific divergence. With a few exceptions, the phylogenetic relationships inferred from the partial rpoB sequences were in agreement with those previously derived from DNA-DNA hybridization studies and analyses of 16S ribosomal DNA gene sequences and partial HSP60 gene sequences. The staphylococcal rpoB sequence database we established enabled us to develop a molecular method for identifying Staphylococcus isolates by PCR followed by direct sequencing of the 751-bp amplicon. In blind tests, this method correctly identified 10 Staphylococcus isolates, and no positive results were obtained with 10 non-Staphylococcus gram-positive and gram-negative bacterial isolates. We propose partial sequencing of the rpoB gene as a new tool for the accurate identification of Staphylococcus isolates.
URL: 11923353
Data: M. T. Parker, PHLS Carmarthen in 1948 / Ice cream
Accession Date: 01/01/1948
Authority: (Fairbrother 1940) Shaw et al. 1951 (AL)
Depositor: PARKER M T
Taxonomy: TaxLink: S5392 (Staphylococcus saprophyticus subsp. saprophyticus (Fairbrother 1940) Shaw et al. 1951) - Date of change: 5/02/2003
Biosafety Responsibility: It is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country

Additional Information

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The Culture Collections hold cell cultures, bacteria, fungi and virus strains from worldwide sources. Our scientists ensure that the identification of the cultures is correct and they remain unchanged from when they are first deposited with the Collection. Nevertheless, some of the data we provide about the cultures is supplied by the person depositing the strains and, although we have multiple checking procedures in place, we cannot always verify all their data. Please note that the Culture Collections cannot be held responsible for any inaccuracies in the data provided by the depositors.

Cultures supplied by Culture Collections are to be used as controls for microbiology testing and for research purposes only. Please view the Terms & Conditions of Supply for more information.

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Available Formats

Ampoule (Bacteria)

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