Extended Bibliography: |
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Ref #: |
19216 |
Author(s): |
Greisen,K.;Loeffelholz,M.;Purohit,A.;Leong,D. |
Journal: |
J Clin Microbiol |
Title: |
PCR primers and probes for the 16S rRNA gene of most species of pathogenic bacteria, including bacteria found in cerebrospinal fluid |
Volume: |
32 |
Page(s): |
335-51 |
Year: |
1994 |
Keyword(s): |
GENBANK/U02893
GENBANK/U02894
GENBANK/U02895
GENBANK/U02896
GENBANK/U02897
GENBANK/U02898
GENBANK/U02899
GENBANK/U02900
GENBANK/U02901
GENBANK/U02902
GENBANK/U02903
GENBANK/U02904
GENBANK/U02905
GENBANK/U02906
GENBANK/U02907
GENBANK/U02908
GENBANK/U02909
GENBANK/U02910
GENBANK/U02911
GENBANK/U02912
GENBANK/U02913
GENBANK/U02914
GENBANK/U02915
GENBANK/U02916
GENBANK/U02917
GENBANK/U02918
GENBANK/U02919
GENBANK/U02920
GENBANK/U02921
GENBANK/U02922
Bacteremia/diagnosis/microbiology
Bacteria/*genetics/isolation & purification/pathogenicity
Bacterial Infections/diagnosis/microbiology
Base Sequence
Cerebrospinal Fluid/microbiology
DNA Primers/genetics
DNA Probes/genetics
Genes, Bacterial
Gram-Negative Bacteria/genetics
Gram-Positive Bacteria/genetics
Humans
Meningitis, Bacterial/diagnosis/microbiology
Molecular Sequence Data
Nucleic Acid Hybridization
*Polymerase Chain Reaction/statistics & numerical data
RNA, Bacterial/*genetics
RNA, Ribosomal, 16S/*genetics
Sensitivity and Specificity
Sequence Homology, Nucleic Acid
Species Specificity
|
Remarks: |
A set of broad-range PCR primers for the 16S rRNA gene in bacteria were tested, along with three series of oligonucleotide probes to detect the PCR product. The first series of probes is broad in range and consists of a universal bacterial probe, a gram-positive probe, a Bacteroides-Flavobacterium probe, and two probes for other gram-negative species. The second series was designed to detect PCR products from seven major bacterial species or groups frequently causing meningitis: Neisseria meningitidis, Haemophilus influenzae, Streptococcus pneumoniae, S. agalactiae, Escherichia coli and other enteric bacteria, Listeria monocytogenes, and Staphylococcus aureus. The third series was designed for the detection of DNA from species or genera commonly considered potential contaminants of clinical samples, including cerebrospinal fluid (CSF): Bacillus, Corynebacterium, Propionibacterium, and coagulase-negative Staphylococcus spp. The primers amplified DNA from all 124 different species of bacteria tested. Southern hybridization testing of the broad-range probes with washes containing 3 M tetramethylammonium chloride indicated that this set of probes correctly identified all but two of the 102 bacterial species tested, the exceptions being Deinococcus radiopugnans and Gardnerella vaginalis. The gram-negative and gram-positive probes hybridized to isolates of two newly characterized bacteria, Alloiococcus otitis and Rochalimaea henselii, as predicted by Gram stain characteristics. The CSF pathogen and contaminant probe sequences were compared with available sequence information and with sequencing data for 32 different species. Testing of the CSF pathogen and contaminant probes against DNA from over 60 different strains indicated that, with the exception of the coagulase-negative Staphylococcus probes, these probes provided the correct identification of bacterial species known to be found in CSF. |
URL: |
7512093 |
|
Ref #: |
80893 |
Author(s): |
Kunishima,S.;Inoue,C.;Kamiya,T.;Ozawa,K. |
Journal: |
Transfusion |
Title: |
Presence of Propionibacterium acnes in blood components |
Volume: |
41 |
Page(s): |
1126-9 |
Year: |
2001 |
Keyword(s): |
Erythrocytes/*microbiology
Humans
Polymerase Chain Reaction
Propionibacterium acnes/genetics/*isolation & purification
Sequence Homology, Nucleic Acid
Staphylococcus/isolation & purification
|
Remarks: |
BACKGROUND: Sterility testing, as part of the QC of blood components at the Japanese Red Cross Aichi Blood Center between April 1998 and March 2000, showed that 10 of 5568 tested blood components (0.18%), all of which were RBC concentrates, were contaminated with bacteria. Nine isolates were Propionibacterium acnes and one was Staphylococcus capitis. STUDY DESIGN AND METHODS: To investigate the molecular relatedness of eight available P. acnes isolates, 16S rRNA gene analysis and random amplified polymorphic DNA (RAPD) analysis were performed. RESULTS: DNA sequencing analysis of the 16S rRNA gene showed that five isolates were identified as distinct strains and that three had identical sequences. RAPD analysis in the latter three isolates showed that two exhibited indistinguishable banding patterns that differed from that of the third isolate. CONCLUSION: P. acnes was the most frequent contaminant of blood components, and six of eight isolates were molecularly unrelated. Further studies are necessary to investigate the precise mechanisms of contamination. |
URL: |
11552069 |
|
Ref #: |
13687 |
Author(s): |
Greisen,K.;Loeffelholz,M.;Purohit,A.;Leong,D. |
Journal: |
J Clin Microbiol |
Title: |
PCR primers and probes for the 16S rRNA gene of most species of pathogenic |
Volume: |
32 |
Page(s): |
335-351 |
Year: |
1994 |
Keyword(s): |
0 (DNA Primers)
0 (DNA Probes)
0 (RNA, Bacterial)
0 (RNA, Ribosomal, 16S)
Bacteremia/diagnosis/microbiology
Bacteria/*genetics/isolation & purification/pathogenicity
Bacterial Infections/diagnosis/microbiology
Base Sequence
Cerebrospinal Fluid/microbiology
DNA Primers/genetics
DNA Probes/genetics
Genes, Bacterial
Gram-Negative Bacteria/genetics
Gram-Positive Bacteria/genetics
Human
Meningitis, Bacterial/diagnosis/microbiology
Molecular Sequence Data
Nucleic Acid Hybridization
*Polymerase Chain Reaction/statistics & numerical data
RNA, Bacterial/*genetics
RNA, Ribosomal, 16S/*genetics
Sensitivity and Specificity
Sequence Homology, Nucleic Acid
Species Specificity
|
Remarks: |
A set of broad-range PCR primers for the 16S rRNA gene in bacteria were |
URL: |
94201356 |
|
Ref #: |
1300 |
Author(s): |
Skerman,V.B.D.;McGowan,V.;Sneath,P.H.A.(ed) |
Journal: |
Int. J. Syst. Bacteriol. |
Title: |
Approved Lists of Bacterial Names. |
Volume: |
30 |
Page(s): |
225-420 |
Year: |
1980 |
|
Ref #: |
6924 |
Author(s): |
DeutschesInstitutfürNormungDIN.NormenausschußMedizin(NAMed) |
Title: |
DIN 58959-7. Qualitätsmanagement in der medizinischen Mikrobiologie. Teil 7: Allgemeine Anforderungen an das Mitführen von Kontrollstämmen. Beiblatt 2: ATCC- und DSM-Nummern häufig verwendeter Kontrollstämme. |
Year: |
1997 |
|
Ref #: |
907 |
Author(s): |
Johnson,J.L.;Cummins,C.S. |
Journal: |
J. Bacteriol. |
Title: |
Cell wall composition and deoxyribonucleic acid similarities among the anaerobic coryneforms, classical propionibacteria, and strains of Arachnia propionica. |
Volume: |
209 |
Page(s): |
1047-1066 |
Year: |
1972 |
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