Culture Collections

Bacteria and Mycoplasmas detail

Conditions of Supply of Microbial Pathogens: Safety

Bacteria Collection: Lactococcus lactis subsp. lactis

NCTC Number: NCTC 6681
Current Name: Lactococcus lactis subsp. lactis
Original Strain Reference: O J
Other Collection No: ATCC 19435; BUCSAV 302; CCM 1877; DSM 20481; NCDO 604; NCIB 6681; O J; WDCM 00016
Previous Catalogue Name: Streptococcus lactis
Type Strain: Yes
Family: Streptococcaceae
Hazard Group (ACDP): 2
Release Restrictions: Terms & Conditions of Supply of Microbial Pathogens: Safety
Antigenic Properties: serovar group n
Conditions for growth on solid media: Columbia blood agar, 24-48 hours, 37°C, aerobic
Conditions for growth on liquid media: nutrient broth,37, facultative anaerobe
16S rRNA Gene Sequence: >gb|DQ788632|DSM 20481|Lactococcus lactis subsp. lactis strain DSM 20481 16S ribosomal RNAgene, partial sequence; 16S-23S ribosomal RNA intergenic spacer andtRNA-Ala gene, complete sequence; and 23S ribosomal RNA gene,partial sequence.| gaagtcgtaacaagg... >gb|AB100803|NCDO 604T|Lactococcus lactis subsp. lactis gene for 16S rRNA, partialsequence, strain: NCDO 604T.| gacgaacgctggcgg...
23S rRNA Gene Sequence: >gb|X68435|SSP.LACTIS| STRAIN DSM 20481|L.lactis (DSM 20481) gene for 23S rRNA.| ggcaaagttaataag... >gb|DQ788632|DSM 20481|Lactococcus lactis subsp. lactis strain DSM 20481 16S ribosomal RNAgene, partial sequence; 16S-23S ribosomal RNA intergenic spacer andtRNA-Ala gene, complete sequence; and 23S ribosomal RNA gene,partial sequence.| gaagtcgtaacaagg...
Bibliography: SHATTOCK P M F & MATTICK A T R 1943 J HYG CAMB 43 173
Extended Bibliography: showhide Show bibliography
Ref #: 53561
Author(s): Mori,S.;Mori,K.;Suzuki,I.;Kasumi,T.
Journal: Syst Appl Microbiol
Title: Phylogenetic analysis of Lactococcus lactis subspecies based on decoding the sequence of the pepT tripeptidase gene, the pepV dipeptidase gene and 16S rRNA
Volume: 27
Page(s): 414-22
Year: 2004
Keyword(s): GENBANK/AB100761 GENBANK/AB100762 GENBANK/AB100763 GENBANK/AB100764 GENBANK/AB100765 GENBANK/AB100766 GENBANK/AB100767 GENBANK/AB100768 GENBANK/AB100769 GENBANK/AB100770 GENBANK/AB100771 GENBANK/AB100772 GENBANK/AB100773 GENBANK/AB100774 GENBANK/AB100775 GENBANK/AB100776 GENBANK/AB100777 GENBANK/AB100778 GENBANK/AB100779 GENBANK/AB100780 GENBANK/AB100781 GENBANK/AB100782 GENBANK/AB100783 GENBANK/AB100784 GENBANK/AB100785 GENBANK/AB100786 GENBANK/AB100787 GENBANK/AB100788 GENBANK/AB100789 GENBANK/AB100790 GENBANK/AB100791 GENBANK/AB100792 GENBANK/AB100793 GENBANK/AB100794 GENBANK/AB100795 GENBANK/AB100796 GENBANK/AB100797 GENBANK/AB100798 GENBANK/AB100799 GENBANK/AB100800 GENBANK/AB100801 GENBANK/AB100802 GENBANK/AB100803 GENBANK/AB100804 GENBANK/AB100805 Amino Acid Sequence Aminopeptidases/chemistry/*genetics Cloning, Molecular Dipeptidases/chemistry/*genetics Lactococcus lactis/*classification/enzymology/genetics Molecular Sequence Data *Phylogeny Polymerase Chain Reaction RNA, Ribosomal, 16S/*genetics Sequence Analysis, DNA
Remarks: Tripeptidase (PepT) and dipeptidase (PepV), the enzymes located in the final stage of the intracellular proteolytic system, were demonstrated to be distributed widely in lactic acid bacteria, especially in lactococci. Both the tripeptidase genes (pepT) and dipeptidase genes (pepV) of 15 lactococcal strains consisting of the type and domestic strains were cloned and sequenced using normal and TAIL PCR methods. Amino acid sequences of these enzymes were highly conserved among strains. Evolutionary distance trees based on the sequence of 1239 nucleotides of pepT and 1416 nucleotide of pepV showed a similar cluster as that obtained from the 1499 fragment of the 16S rRNA. Based on this profile, the species Lactococcus lactis is reasonably divided into three subspecies groups, subsp. lactis, cremoris, and hordniae, as in the current classification. Figure of trees from pepT and pepV were essentially identical to each other and slightly more intricate than that from 16S rRNA. The K nuc values obtained from pepT and pepV genes were approximately ten times as high as that from 16S rRNA. Considering these results, phylogenetic analysis based on pepT and pepV genes may aid in a more precise index of classification of L. lactis subspecies. PepT and PepV seem to have evolved in similar directions in lactococci.
URL: 15368846
Ref #: 1300
Author(s): Skerman,V.B.D.;McGowan,V.;Sneath,P.H.A.(ed)
Journal: Int. J. Syst. Bacteriol.
Title: Approved Lists of Bacterial Names.
Volume: 30
Page(s): 225-420
Year: 1980
Ref #: 1322
Author(s): Garvie,E.I.;Farrow,J.A.E.;Phillips,B.A.
Journal: Zbl. Bakt. Hyg., I. Abt. Orig. C
Title: A taxonomic study of some strains of streptococci which grow at 10 C but not at 45 C including Streptococcus lactis and Streptococcus cremoris.
Volume: 2
Page(s): 151-165
Year: 1981
Ref #: 2990
Journal: Int. J. Syst. Bacteriol.
Title: Validation of the publication of new names and new combinations previously effectively published outside the IJSB. List No. 20.
Volume: 36
Page(s): 354-356
Year: 1986
Ref #: 3222
Author(s): Schleifer,K.H.;Kraus,J.;Dvorak,C.;Kilpper-Bälz,R.;Collins,M.D.;Fischer,W.
Journal: System. Appl. Microbiol.
Title: Transfer of Streptococcus lactis and related streptococci to the genus Lactococcus gen. nov.
Volume: 6
Page(s): 183-195
Year: 1985
Data: (ATCC 19435, NCIB 6681, NCDO 604, BUCSAV 302) Type strain / P. M. F. Shattock, NIRD Shinfield in 1941 / Production of group N antiserum / Shattock, P. M. F. & Mattick, A. T. R. (1943) J. Hyg., Camb. 43, 173
Accession Date: 01/01/1941
Authority: (Lister 1873) Schleifer et al. 1986
Depositor: SHATTOCK P M F
Taxonomy: TaxLink: S1622 (Streptococcus lactis lactis) - Date of change: 5/02/2003
Other: Group N Serotype
Biosafety Responsibility: It is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country

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The Culture Collections hold cell cultures, bacteria, fungi and virus strains from worldwide sources. Our scientists ensure that the identification of the cultures is correct and they remain unchanged from when they are first deposited with the Collection. Nevertheless, some of the data we provide about the cultures is supplied by the person depositing the strains and, although we have multiple checking procedures in place, we cannot always verify all their data. Please note that the Culture Collections cannot be held responsible for any inaccuracies in the data provided by the depositors.

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