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Bacteria Collection: Klebsiella pneumoniae subsp. rhinoscleromatis

NCTC Number: NCTC 5046
Current Name: Klebsiella pneumoniae subsp. rhinoscleromatis
Original Strain Reference: R 70
Other Collection No: ATCC 13884; KAUFFMANN C 5046; R 70
Previous Catalogue Name: Klebsiella rhinoscleromatis
Type Strain: Yes
Family: Enterobacteriaceae
Hazard Group (ACDP): 2
Release Restrictions: Terms & Conditions of Supply of Microbial Pathogens: Safety
Conditions for growth on solid media: Nutrient agar, 24 hours, 37°C, aerobic
Conditions for growth on liquid media: nutrient broth,37, facultative anaerobe
Isolated From: human, nose
Whole Genome Sequence: http://www.ebi.ac.uk/ena/data/view/ERS513127
Annotated Genome: ftp://ftp.sanger.ac.uk/pub/project/pathogens/NCTC3000/...
16S rRNA Gene Sequence: >gb|AF130983|ATCC13884|Klebsiella pneumoniae subsp. rhinoscleromatis 16S ribosomal RNAgene, partial sequence.| atcctggctcagatt... >gb|AF009169|NCTC5046|Klebsiella rhinoscleromatis 16S ribosomal RNA gene, partialsequence.| ggcggacgggtgagt... >gb|Y17657|ATCC13884T|Klebsiella pneumoniae 16S rRNA gene, strain ATCC13884T, partial.| tgaacgctggcggca...
Miscellaneous Sequence Data: >gb|AY370850|ATCC 13884|Klebsiella pneumoniae subsp. rhinoscleromatis strain ATCC 13884gyrase B (gyrB) gene, partial cds.| aaagtttccggcggt...
Bibliography: SNIJDERS E P & GOSLINGS W R O 1935 ZENTBL BAKT PARASIT KDE I ABT ORIG 134
Extended Bibliography: showhide Show bibliography
Ref #: 31703
Author(s): Carter,J.S.;Bowden,F.J.;Bastian,I.;Myers,G.M.;Sriprakash,K.S.;Kemp,D.J.
Journal: Int J Syst Bacteriol
Title: Phylogenetic evidence for reclassification of Calymmatobacterium granulomatis as Klebsiella granulomatis comb. nov
Volume: 49 Pt 4
Page(s): 1695-700
Year: 1999
Keyword(s): GENBANK/AF009169 GENBANK/AF009171 GENBANK/AF009230 GENBANK/AF009231 Calymmatobacterium/*classification/cytology/genetics/physiology Escherichia coli Proteins Genes, rRNA Granuloma Inguinale/*microbiology Humans Klebsiella/*classification/cytology/genetics/physiology Molecular Sequence Data Phylogeny Porins/genetics RNA, Ribosomal, 16S/genetics Sequence Analysis, DNA
Remarks: By sequencing a total of 2089 bp of the 16S rRNA and phoE genes it was demonstrated that Calymmatobacterium granulomatis (the causative organism of donovanosis) shows a high level of identity with Klebsiella species pathogenic to humans (Klebsiella pneumoniae, Klebsiella rhinoscleromatis). It is proposed that C. granulomatis should be reclassified as Klebsiella granulomatis comb. nov. An emended description of the genus Klebsiella is given.
URL: 10555350
Ref #: 50886
Author(s): Drancourt,M.;Bollet,C.;Carta,A.;Rousselier,P.
Journal: Int J Syst Evol Microbiol
Title: Phylogenetic analyses of Klebsiella species delineate Klebsiella and Raoultella gen. nov., with description of Raoultella ornithinolytica comb. nov., Raoultella terrigena comb. nov. and Raoultella planticola comb. nov
Volume: 51
Page(s): 925-32
Year: 2001
Keyword(s): GENBANK/AF129441 GENBANK/AF129442 GENBANK/AF129443 DNA, Bacterial/genetics DNA, Ribosomal/genetics DNA-Directed RNA Polymerases/genetics Enterobacter aerogenes/classification/genetics Enterobacteriaceae/*classification/genetics Erwinia/classification/genetics Klebsiella/*classification/genetics Molecular Sequence Data Nucleic Acid Hybridization *Phylogeny RNA, Ribosomal, 16S/genetics
Remarks: The phylogenetic relationships of the type strains of 9 Klebsiella species and 20 species from 11 genera of the family Enterobacteriaceae were investigated by performing a comparative analysis of the sequences of the 16S rRNA and rpoB genes. The sequence data were phylogenetically analysed by the neighbourjoining and parsimony methods. The phylogenetic inference of the sequence comparison confirmed that the genus Klebsiella is heterogeneous and composed of species which form three clusters that also included members of other genera, including Enterobacter aerogenes, Erwinia clusters I and II and Tatumella. Cluster I contained the type strains of Klebsiella pneumoniae subsp. pneumoniae, Klebsiella pneumoniae subsp. rhinoscleromatis and Klebsiella pneumoniae subsp. ozaenae. Cluster II contained Klebsiella ornithinolytica, Klebsiella planticola, Klebsiella trevisanii and Klebsiella terrigena, organisms characterized by growth at 10 degrees C and utilization of L-sorbose as carbon source. Cluster III contained Klebsiella oxytoca. The data from the sequence analyses along with previously reported biochemical and DNA-DNA hybridization data support the division of the genus Klebsiella into two genera and one genogroup. The name Raoultella is proposed as a genus name for species of cluster II and emended definitions of Klebsiella species are proposed.
URL: 11411716
Ref #: 43181
Author(s): Delmas,J.;Breysse,F.;Devulder,G.;Flandrois,J.P.;Chomarat,M.
Journal: Diagn Microbiol Infect Dis
Title: Rapid identification of Enterobacteriaceae by sequencing DNA gyrase subunit B encoding gene
Volume: 55
Page(s): 263-8
Year: 2006
Keyword(s): Bacterial Typing Techniques/*methods DNA Gyrase/*genetics Enterobacteriaceae/enzymology/genetics/*isolation & purification Genotype Humans Sequence Analysis, DNA/methods
Remarks: Real-time polymerase chain reaction and sequencing were used to characterize a 506-bp-long DNA fragment internal to the gyrB gene (gyrBint). The sequences obtained from 32 Enterobacteriaceae-type strains and those available in the Genbank nucleotide sequence database (n = 24) were used as a database to identify 240 clinical enterobacteria isolates. Sequence analysis of the gyrBint fragment of 240 strains showed that gyrBint constitutes a discriminative target sequence to differentiate between Enterobacteriaceae species. Comparison of these identifications with those obtained by phenotypic methods (Vitek 1 system and/or Rapid ID 32E; bioMerieux, Marcy l'Etoile, France) revealed discrepancies essentially with genera Citrobacter and Enterobacter. Most of the strains identified as Enterobacter cloacae by phenotypic methods were identified as Enterobacter hormaechei strains by gyrBint sequencing. The direct sequencing of gyrBint would be useful as a complementary tool in the identification of clinical Enterobacteriaceae isolates.
URL: 16626902
Ref #: 12919
Author(s): Carter,J.S.;Bowden,F.J.;Bastian,I.;Myers,G.M.;Sriprakash,K.S.;Kemp,D.J.
Journal: Int J Syst Bacteriol
Title: Phylogenetic evidence for reclassification of Calymmatobacterium granulomatis as Klebsiella granulomatis comb. nov
Volume: 49 Pt 4
Page(s): 1695-700
Year: 1999
Keyword(s): GENBANK/AF009169 GENBANK/AF009171 GENBANK/AF009230 GENBANK/AF009231 Calymmatobacterium/*classification/cytology/genetics/physiology Genes, rRNA Granuloma Inguinale/*microbiology Human Klebsiella/*classification/cytology/genetics/physiology Molecular Sequence Data Phylogeny Porins/genetics RNA, Ribosomal, 16S/genetics Sequence Analysis, DNA Support, Non-U.S. Gov't
Remarks: By sequencing a total of 2089 bp of the 16S rRNA and phoE genes it was demonstrated that Calymmatobacterium granulomatis (the causative organism of donovanosis) shows a high level of identity with Klebsiella species pathogenic to humans (Klebsiella pneumoniae, Klebsiella rhinoscleromatis). It is proposed that C. granulomatis should be reclassified as Klebsiella granulomatis comb. nov. An emended description of the genus Klebsiella is given.
URL: 20023050
Ref #: 12920
Author(s): Drancourt,M.;Bollet,C.;Carta,A.;Rousselier,P.
Journal: Int J Syst Evol Microbiol
Title: Phylogenetic analyses of Klebsiella species delineate Klebsiella and Raoultella gen. nov., with description of Raoultella ornithinolytica comb. nov., Raoultella terrigena comb. nov. and Raoultella planticola comb. nov
Volume: 51
Page(s): 925-32
Year: 2001
Keyword(s): GENBANK/AF129441 GENBANK/AF129442 GENBANK/AF129443 DNA, Bacterial/genetics DNA, Ribosomal/genetics DNA-Directed RNA Polymerases/genetics Enterobacter aerogenes/classification/genetics Enterobacteriaceae/*classification/genetics Erwinia/classification/genetics Klebsiella/*classification/genetics Molecular Sequence Data Nucleic Acid Hybridization *Phylogeny RNA, Ribosomal, 16S/genetics
Remarks: The phylogenetic relationships of the type strains of 9 Klebsiella species and 20 species from 11 genera of the family Enterobacteriaceae were investigated by performing a comparative analysis of the sequences of the 16S rRNA and rpoB genes. The sequence data were phylogenetically analysed by the neighbourjoining and parsimony methods. The phylogenetic inference of the sequence comparison confirmed that the genus Klebsiella is heterogeneous and composed of species which form three clusters that also included members of other genera, including Enterobacter aerogenes, Erwinia clusters I and II and Tatumella. Cluster I contained the type strains of Klebsiella pneumoniae subsp. pneumoniae, Klebsiella pneumoniae subsp. rhinoscleromatis and Klebsiella pneumoniae subsp. ozaenae. Cluster II contained Klebsiella ornithinolytica, Klebsiella planticola, Klebsiella trevisanii and Klebsiella terrigena, organisms characterized by growth at 10 degrees C and utilization of L-sorbose as carbon source. Cluster III contained Klebsiella oxytoca. The data from the sequence analyses along with previously reported biochemical and DNA-DNA hybridization data support the division of the genus Klebsiella into two genera and one genogroup. The name Raoultella is proposed as a genus name for species of cluster II and emended definitions of Klebsiella species are proposed.
URL: 21304435
Data: (Kauffmann C 5046, ATCC 13884) Type strain / E. P. Snijders, Amsterdam in 1937 / Nose, Sumatra / Capsular serotype 3 / Snijders, E. P. & Goslings, W. R. O. (1934) Zentbl. Bakt. ParasitKde, I. Abt. Orig. 132, 343; (1934) 133, 33; (1935) 134, 196; (1936) 136, 1, / Cowan, S. T. et al. (1960) J. gen. Microbiol. 23, 601
Accession Date: 01/01/1937
History: ISOLATED BY SNIJDERS E P AMSTERDAM
Authority: (Trevisan 1887) Oerskov 1984
Depositor: SNIJDERS E P
Taxonomy: TaxLink: S1520 (Klebsiella rhinoscleromatis rhinoscleromatis) - Date of change: 5/02/2003
Biosafety Responsibility: It is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country

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