Bacteria and Mycoplasmas detail

Conditions of Supply of Microbial Pathogens: Safety

Bacteria Collection: Klebsiella pneumoniae subsp. rhinoscleromatis

NCTC Number:

NCTC 5046

Current Name:

Klebsiella pneumoniae subsp. rhinoscleromatis

Original Strain Reference:

R 70

Other Collection No:

ATCC 13884; KAUFFMANN C 5046; R 70

Previous Catalogue Name:

Klebsiella rhinoscleromatis

Type Strain:

Yes

Family:

Enterobacteriaceae

Hazard Group (ACDP):

Release Restrictions:

Terms & Conditions of Supply of Microbial Pathogens: Safety

Conditions for growth on solid media:

Nutrient agar, 24 hours, 37°C, aerobic

Conditions for growth on liquid media:

nutrient broth,37, facultative anaerobe

Isolated From:

human, nose

Whole Genome Sequence:

http://www.ebi.ac.uk/ena/data/view/ERS513127

Annotated Genome:

ftp://ftp.sanger.ac.uk/pub/project/pathogens/NCTC3000/...

16S rRNA Gene Sequence:

Miscellaneous Sequence Data:

>gb|AY370850|ATCC 13884|Klebsiella pneumoniae subsp. rhinoscleromatis strain ATCC 13884gyrase B (gyrB) gene, partial cds.| aaagtttccggcggt...

Bibliography:

SNIJDERS E P & GOSLINGS W R O 1935 ZENTBL BAKT PARASIT KDE I ABT ORIG 134

Extended Bibliography:

Show bibliography

Ref #:	31703
Author(s):	Carter,J.S.;Bowden,F.J.;Bastian,I.;Myers,G.M.;Sriprakash,K.S.;Kemp,D.J.
Journal:	Int J Syst Bacteriol
Title:	Phylogenetic evidence for reclassification of Calymmatobacterium granulomatis as Klebsiella granulomatis comb. nov
Volume:	49 Pt 4
Page(s):	1695-700
Year:	1999
Keyword(s):	GENBANK/AF009169 GENBANK/AF009171 GENBANK/AF009230 GENBANK/AF009231 Calymmatobacterium/classification/cytology/genetics/physiology Escherichia coli Proteins Genes, rRNA Granuloma Inguinale/microbiology Humans Klebsiella/*classification/cytology/genetics/physiology Molecular Sequence Data Phylogeny Porins/genetics RNA, Ribosomal, 16S/genetics Sequence Analysis, DNA
Remarks:	By sequencing a total of 2089 bp of the 16S rRNA and phoE genes it was demonstrated that Calymmatobacterium granulomatis (the causative organism of donovanosis) shows a high level of identity with Klebsiella species pathogenic to humans (Klebsiella pneumoniae, Klebsiella rhinoscleromatis). It is proposed that C. granulomatis should be reclassified as Klebsiella granulomatis comb. nov. An emended description of the genus Klebsiella is given.
URL:	10555350

Ref #:	50886
Author(s):	Drancourt,M.;Bollet,C.;Carta,A.;Rousselier,P.
Journal:	Int J Syst Evol Microbiol
Title:	Phylogenetic analyses of Klebsiella species delineate Klebsiella and Raoultella gen. nov., with description of Raoultella ornithinolytica comb. nov., Raoultella terrigena comb. nov. and Raoultella planticola comb. nov
Volume:	51
Page(s):	925-32
Year:	2001
Keyword(s):	GENBANK/AF129441 GENBANK/AF129442 GENBANK/AF129443 DNA, Bacterial/genetics DNA, Ribosomal/genetics DNA-Directed RNA Polymerases/genetics Enterobacter aerogenes/classification/genetics Enterobacteriaceae/classification/genetics Erwinia/classification/genetics Klebsiella/classification/genetics Molecular Sequence Data Nucleic Acid Hybridization *Phylogeny RNA, Ribosomal, 16S/genetics
Remarks:	The phylogenetic relationships of the type strains of 9 Klebsiella species and 20 species from 11 genera of the family Enterobacteriaceae were investigated by performing a comparative analysis of the sequences of the 16S rRNA and rpoB genes. The sequence data were phylogenetically analysed by the neighbourjoining and parsimony methods. The phylogenetic inference of the sequence comparison confirmed that the genus Klebsiella is heterogeneous and composed of species which form three clusters that also included members of other genera, including Enterobacter aerogenes, Erwinia clusters I and II and Tatumella. Cluster I contained the type strains of Klebsiella pneumoniae subsp. pneumoniae, Klebsiella pneumoniae subsp. rhinoscleromatis and Klebsiella pneumoniae subsp. ozaenae. Cluster II contained Klebsiella ornithinolytica, Klebsiella planticola, Klebsiella trevisanii and Klebsiella terrigena, organisms characterized by growth at 10 degrees C and utilization of L-sorbose as carbon source. Cluster III contained Klebsiella oxytoca. The data from the sequence analyses along with previously reported biochemical and DNA-DNA hybridization data support the division of the genus Klebsiella into two genera and one genogroup. The name Raoultella is proposed as a genus name for species of cluster II and emended definitions of Klebsiella species are proposed.
URL:	11411716

Ref #:	43181
Author(s):	Delmas,J.;Breysse,F.;Devulder,G.;Flandrois,J.P.;Chomarat,M.
Journal:	Diagn Microbiol Infect Dis
Title:	Rapid identification of Enterobacteriaceae by sequencing DNA gyrase subunit B encoding gene
Volume:	55
Page(s):	263-8
Year:	2006
Keyword(s):	Bacterial Typing Techniques/methods DNA Gyrase/genetics Enterobacteriaceae/enzymology/genetics/*isolation & purification Genotype Humans Sequence Analysis, DNA/methods
Remarks:	Real-time polymerase chain reaction and sequencing were used to characterize a 506-bp-long DNA fragment internal to the gyrB gene (gyrBint). The sequences obtained from 32 Enterobacteriaceae-type strains and those available in the Genbank nucleotide sequence database (n = 24) were used as a database to identify 240 clinical enterobacteria isolates. Sequence analysis of the gyrBint fragment of 240 strains showed that gyrBint constitutes a discriminative target sequence to differentiate between Enterobacteriaceae species. Comparison of these identifications with those obtained by phenotypic methods (Vitek 1 system and/or Rapid ID 32E; bioMerieux, Marcy l'Etoile, France) revealed discrepancies essentially with genera Citrobacter and Enterobacter. Most of the strains identified as Enterobacter cloacae by phenotypic methods were identified as Enterobacter hormaechei strains by gyrBint sequencing. The direct sequencing of gyrBint would be useful as a complementary tool in the identification of clinical Enterobacteriaceae isolates.
URL:	16626902

Ref #:	12919
Author(s):	Carter,J.S.;Bowden,F.J.;Bastian,I.;Myers,G.M.;Sriprakash,K.S.;Kemp,D.J.
Journal:	Int J Syst Bacteriol
Title:	Phylogenetic evidence for reclassification of Calymmatobacterium granulomatis as Klebsiella granulomatis comb. nov
Volume:	49 Pt 4
Page(s):	1695-700
Year:	1999
Keyword(s):	GENBANK/AF009169 GENBANK/AF009171 GENBANK/AF009230 GENBANK/AF009231 Calymmatobacterium/classification/cytology/genetics/physiology Genes, rRNA Granuloma Inguinale/microbiology Human Klebsiella/*classification/cytology/genetics/physiology Molecular Sequence Data Phylogeny Porins/genetics RNA, Ribosomal, 16S/genetics Sequence Analysis, DNA Support, Non-U.S. Gov't
Remarks:	By sequencing a total of 2089 bp of the 16S rRNA and phoE genes it was demonstrated that Calymmatobacterium granulomatis (the causative organism of donovanosis) shows a high level of identity with Klebsiella species pathogenic to humans (Klebsiella pneumoniae, Klebsiella rhinoscleromatis). It is proposed that C. granulomatis should be reclassified as Klebsiella granulomatis comb. nov. An emended description of the genus Klebsiella is given.
URL:	20023050

Ref #:	12920
Author(s):	Drancourt,M.;Bollet,C.;Carta,A.;Rousselier,P.
Journal:	Int J Syst Evol Microbiol
Title:	Phylogenetic analyses of Klebsiella species delineate Klebsiella and Raoultella gen. nov., with description of Raoultella ornithinolytica comb. nov., Raoultella terrigena comb. nov. and Raoultella planticola comb. nov
Volume:	51
Page(s):	925-32
Year:	2001
Keyword(s):	GENBANK/AF129441 GENBANK/AF129442 GENBANK/AF129443 DNA, Bacterial/genetics DNA, Ribosomal/genetics DNA-Directed RNA Polymerases/genetics Enterobacter aerogenes/classification/genetics Enterobacteriaceae/classification/genetics Erwinia/classification/genetics Klebsiella/classification/genetics Molecular Sequence Data Nucleic Acid Hybridization *Phylogeny RNA, Ribosomal, 16S/genetics
Remarks:	The phylogenetic relationships of the type strains of 9 Klebsiella species and 20 species from 11 genera of the family Enterobacteriaceae were investigated by performing a comparative analysis of the sequences of the 16S rRNA and rpoB genes. The sequence data were phylogenetically analysed by the neighbourjoining and parsimony methods. The phylogenetic inference of the sequence comparison confirmed that the genus Klebsiella is heterogeneous and composed of species which form three clusters that also included members of other genera, including Enterobacter aerogenes, Erwinia clusters I and II and Tatumella. Cluster I contained the type strains of Klebsiella pneumoniae subsp. pneumoniae, Klebsiella pneumoniae subsp. rhinoscleromatis and Klebsiella pneumoniae subsp. ozaenae. Cluster II contained Klebsiella ornithinolytica, Klebsiella planticola, Klebsiella trevisanii and Klebsiella terrigena, organisms characterized by growth at 10 degrees C and utilization of L-sorbose as carbon source. Cluster III contained Klebsiella oxytoca. The data from the sequence analyses along with previously reported biochemical and DNA-DNA hybridization data support the division of the genus Klebsiella into two genera and one genogroup. The name Raoultella is proposed as a genus name for species of cluster II and emended definitions of Klebsiella species are proposed.
URL:	21304435

Data:

(Kauffmann C 5046, ATCC 13884) Type strain / E. P. Snijders, Amsterdam in 1937 / Nose, Sumatra / Capsular serotype 3 / Snijders, E. P. & Goslings, W. R. O. (1934) Zentbl. Bakt. ParasitKde, I. Abt. Orig. 132, 343; (1934) 133, 33; (1935) 134, 196; (1936) 136, 1, / Cowan, S. T. et al. (1960) J. gen. Microbiol. 23, 601

Accession Date:

01/01/1937

History:

ISOLATED BY SNIJDERS E P AMSTERDAM

Authority:

(Trevisan 1887) Oerskov 1984

Depositor:

SNIJDERS E P

Taxonomy:

TaxLink: S1520 (Klebsiella rhinoscleromatis rhinoscleromatis) - Date of change: 5/02/2003

Biosafety Responsibility:

It is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country

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Note:

The Culture Collections hold cell cultures, bacteria, fungi and virus strains from worldwide sources. Our scientists ensure that the identification of the cultures is correct and they remain unchanged from when they are first deposited with the Collection. Nevertheless, some of the data we provide about the cultures is supplied by the person depositing the strains and, although we have multiple checking procedures in place, we cannot always verify all their data. Please note that the Culture Collections cannot be held responsible for any inaccuracies in the data provided by the depositors.

Cultures supplied by Culture Collections are to be used as controls for microbiology testing and for research purposes only. Please view the Terms & Conditions of Supply for more information.