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Bacteria Collection: Actinobacillus lignieresii

NCTC Number: NCTC 4189
Current Name: Actinobacillus lignieresii
Original Strain Reference: 1
Other Collection No: ATCC 49236; CCUG 41384; CIP 53.130
Previous Catalogue Name: Actinobacillus lignieresii
Type Strain: Yes
Family: Pasteurellaceae
Hazard Group (ACDP): 2
Release Restrictions: Terms & Conditions of Supply of Microbial Pathogens: Safety
Conditions for growth on solid media: Columbia blood agar, 24-48 hours, 37°C, aerobic
Conditions for growth on liquid media: nutrient broth,37, facultative anaerobe
Isolated From: mammal, sub-maxillary gland of cattle
Whole Genome Sequence:
16S rRNA Gene Sequence: >gb|AY362892|NCTC 4189|Actinobacillus lignieresii strain NCTC 4189 16S ribosomal RNA gene,partial sequence.| attgaacgctggcgg...
Miscellaneous Sequence Data: >gb|AY258261|NCTC4189|Actinobacillus lignieresii GyrB (gyrB) gene, partial cds.| ctcctataaagtatc...
Bibliography: THOMPSON L 1933 J INFECT DIS 52 223
Extended Bibliography: showhide Show bibliography
Ref #: 50372
Author(s): Angen,O.;Ahrens,P.;Kuhnert,P.;Christensen,H.;Mutters,R.
Journal: Int J Syst Evol Microbiol
Title: Proposal of Histophilus somni gen. nov., sp. nov. for the three species incertae sedis 'Haemophilus somnus', 'Haemophilus agni' and 'Histophilus ovis'
Volume: 53
Page(s): 1449-56
Year: 2003
Keyword(s): GENBANK/AF549387 GENBANK/AF549388 GENBANK/AF549389 GENBANK/AF549390 GENBANK/AF549391 GENBANK/AF549392 GENBANK/AF549393 GENBANK/AF549394 GENBANK/AF549395 GENBANK/AF549396 GENBANK/AF549397 GENBANK/AF549398 GENBANK/AF549399 GENBANK/AF549400 GENBANK/AF549401 GENBANK/AF549402 GENBANK/AF549403 GENBANK/AF549404 GENBANK/AF549405 GENBANK/AY170207 GENBANK/AY170208 GENBANK/AY170209 GENBANK/AY170210 GENBANK/AY170211 GENBANK/AY170212 GENBANK/AY170213 GENBANK/AY170214 GENBANK/AY170215 GENBANK/AY170216 GENBANK/AY170217 GENBANK/AY170218 GENBANK/AY170219 DNA, Bacterial/genetics DNA, Ribosomal/genetics DNA-Directed RNA Polymerases/genetics Genes, Bacterial Haemophilus/*classification/genetics/metabolism Molecular Sequence Data Pasteurellaceae/*classification/genetics/metabolism Phenotype Phylogeny RNA, Bacterial/genetics RNA, Ribosomal, 16S/genetics Species Specificity
Remarks: Earlier investigations have shown that 'Haemophilus somnus', 'Haemophilus agni' and 'Histophilus ovis' represent the same species. In the present investigation, the taxonomic position of this species is explored further by sequencing the 16S rRNA and rpoB genes of strains that were investigated previously by DNA-DNA hybridization. These results clearly support the allocation of this species to a novel genus within the family PASTEURELLACEAE: The phenotypic separation of Histophilus somni gen. nov., sp. nov. from other members of the family can, for most strains, be based on capnophilia, yellowish pigmentation and indole production. However, due to phenotypic variation, the use of a species-specific PCR test based on the 16S rRNA gene is included in the species description. This is justified by the high sequence similarity of the 16S rRNA gene within the species and the fact that the highest sequence similarity to any other taxon within the family is 93.4 %. The type strain, 8025(T)=ATCC 43625(T)=CCUG 36157(T), was isolated in the USA from a bovine brain with lesions of thromboembolic meningoencephalitis.
URL: 13130032
Ref #: 95477
Author(s): Korczak,B.;Christensen,H.;Emler,S.;Frey,J.;Kuhnert,P.
Journal: Int J Syst Evol Microbiol
Title: Phylogeny of the family Pasteurellaceae based on rpoB sequences
Volume: 54
Page(s): 1393-9
Year: 2004
Keyword(s): Bacterial Proteins/genetics DNA, Bacterial/chemistry/isolation & purification DNA, Ribosomal/chemistry DNA-Directed RNA Polymerases/*genetics Genes, rRNA Molecular Sequence Data Pasteurellaceae/*classification/*genetics Phylogeny RNA, Bacterial/genetics RNA, Ribosomal, 16S/genetics Sequence Analysis, DNA Sequence Homology
Remarks: Sequences of the gene encoding the beta-subunit of the RNA polymerase (rpoB) were used to delineate the phylogeny of the family Pasteurellaceae. A total of 72 strains, including the type strains of the major described species as well as selected field isolates, were included in the study. Selection of universal rpoB-derived primers for the family allowed straightforward amplification and sequencing of a 560 bp fragment of the rpoB gene. In parallel, 16S rDNA was sequenced from all strains. The phylogenetic tree obtained with the rpoB sequences reflected the major branches of the tree obtained with the 16S rDNA, especially at the genus level. Only a few discrepancies between the trees were observed. In certain cases the rpoB phylogeny was in better agreement with DNA-DNA hybridization studies than the phylogeny derived from 16S rDNA. The rpoB gene is strongly conserved within the various species of the family of Pasteurellaceae. Hence, rpoB gene sequence analysis in conjunction with 16S rDNA sequencing is a valuable tool for phylogenetic studies of the Pasteurellaceae and may also prove useful for reorganizing the current taxonomy of this bacterial family.
URL: 15280320
Ref #: 47767
Author(s): Christensen,H.;Kuhnert,P.;Olsen,J.E.;Bisgaard,M.
Journal: Int J Syst Evol Microbiol
Title: Comparative phylogenies of the housekeeping genes atpD, infB and rpoB and the 16S rRNA gene within the Pasteurellaceae
Volume: 54
Page(s): 1601-9
Year: 2004
Keyword(s): GENBANK/AY296207 GENBANK/AY296208 GENBANK/AY296209 GENBANK/AY296210 GENBANK/AY296211 GENBANK/AY296212 GENBANK/AY296213 GENBANK/AY296214 GENBANK/AY296215 GENBANK/AY296216 GENBANK/AY296217 GENBANK/AY296218 GENBANK/AY296219 GENBANK/AY296220 GENBANK/AY296221 GENBANK/AY296222 GENBANK/AY296223 GENBANK/AY296224 GENBANK/AY296225 GENBANK/AY296226 GENBANK/AY296227 GENBANK/AY296228 GENBANK/AY296229 GENBANK/AY296230 GENBANK/AY296231 GENBANK/AY296232 GENBANK/AY296233 GENBANK/AY296234 GENBANK/AY296235 GENBANK/AY296236 GENBANK/AY296237 GENBANK/AY296238 GENBANK/AY296239 GENBANK/AY296240 GENBANK/AY296241 GENBANK/AY296242 GENBANK/AY296243 GENBANK/AY296244 GENBANK/AY296245 GENBANK/AY296246 GENBANK/AY314025 GENBANK/AY314043 Actinobacillus/genetics Bacterial Proteins/genetics DNA, Bacterial/chemistry/isolation & purification DNA, Ribosomal/chemistry/isolation & purification DNA-Directed RNA Polymerases/*genetics Evolution Genes, Bacterial Genes, rRNA Haemophilus/genetics Mannheimia/genetics Molecular Sequence Data Pasteurella/genetics Pasteurellaceae/*classification/*genetics Phylogeny Prokaryotic Initiation Factor-2/*genetics RNA, Bacterial/genetics RNA, Ribosomal, 16S/*genetics Sequence Analysis, DNA Transcription Factors/*genetics
Remarks: Phylogenies of housekeeping gene and 16S rRNA gene sequences were compared to improve the classification of the bacterial family Pasteurellaceae and knowledge of the evolutionary relationships of its members. Deduced partial protein sequences of the housekeeping genes atpD, infB and rpoB were compared in 28, 36 and 28 representative taxa of the Pasteurellaceae, respectively. The monophyly of representatives of the genus Gallibacterium was recognized by analysis of all housekeeping genes, while members of Mannheimia, Actinobacillus sensu stricto and the core group of Pasteurella sensu stricto formed monophyletic groups with two out of three housekeeping genes. Representatives of Mannheimia, Actinobacillus sensu stricto, [Haemophilus] ducreyi and [Pasteurella] trehalosi formed a monophyletic unit by analysis of all three housekeeping genes, which was in contrast to the 16S rRNA gene-derived phylogeny, where these taxa occurred at separate positions in the phylogenetic tree. Representatives of the Rodent, Avian and Aphrophilus-Haemophilus 16S rRNA gene groups were weakly supported by phylogenetic analysis of housekeeping genes. Phylogenies derived by comparison of the housekeeping genes diverged significantly from the 16S rRNA gene-derived phylogeny as evaluated by the likelihood ratio test. A low degree of congruence was also observed between the individual housekeeping gene-derived phylogenies. Estimates on speciation derived from 16S rRNA and housekeeping gene sequence comparisons resulted in quite different evolutionary scenarios for members of the Pasteurellaceae. The phylogeny based on the housekeeping genes supported observed host associations between Mannheimia, Actinobacillus sensu stricto and [Pasteurella] trehalosi and animals with paired hooves.
URL: 15388716
Data: Type strain / L. Thompsom, Rochester USA in 1933 / Glands, cattle
Accession Date: 01/01/1933
Authority: Brumpt 1910 (AL)
Depositor: THOMPSON L
Taxonomy: TaxLink: S82 (Actinobacillus lignieresii Brumpt 1910) - Date of change: 5/02/2003
Biosafety Responsibility: It is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country

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