| Extended Bibliography: |
Show bibliography
| Ref #: |
84000 |
| Author(s): |
Hassan,A.A.;Khan,I.U.;Abdulmawjood,A.;Lammler,C. |
| Journal: |
J Clin Microbiol |
| Title: |
Evaluation of PCR methods for rapid identification and differentiation of Streptococcus uberis and Streptococcus parauberis |
| Volume: |
39 |
| Page(s): |
1618-21 |
| Year: |
2001 |
| Keyword(s): |
GENBANK/AF255656
GENBANK/AF255657
Animals
Base Sequence
Cattle
Cattle Diseases/microbiology
DNA, Ribosomal Spacer/*genetics
*Genes, rRNA
Mastitis, Bovine/*microbiology
Molecular Sequence Data
Polymerase Chain Reaction/*methods
RNA, Ribosomal, 16S/genetics
RNA, Ribosomal, 23S/genetics
Sequence Analysis, DNA
Streptococcal Infections/microbiology/*veterinary
Streptococcus/*classification/genetics
Time Factors
|
| Remarks: |
Streptococcus uberis and Streptococcus parauberis reference strains and isolates obtained from routine diagnostics were investigated by PCR with oligonucleotide primers designed according to species-specific parts of the 16S rRNA gene, the 23S rRNA gene, and the 16S-23S rRNA intergenic spacer region of both species. All three primer pairs allowed an identification of 67 isolates as S. uberis and 4 isolates as S. parauberis. |
| URL: |
11283100 |
|
| Ref #: |
84005 |
| Author(s): |
Harland,N.M.;Leigh,J.A.;Collins,M.D. |
| Journal: |
J Appl Bacteriol |
| Title: |
Development of gene probes for the specific identification of Streptococcus uberis and Streptococcus parauberis based upon large subunit rRNA gene sequences |
| Volume: |
74 |
| Page(s): |
526-31 |
| Year: |
1993 |
| Keyword(s): |
Bacterial Typing Techniques
Base Sequence
DNA, Bacterial/genetics
DNA, Ribosomal/*genetics
Genes, Bacterial
Molecular Sequence Data
Nucleic Acid Hybridization
*Oligonucleotide Probes
Polymerase Chain Reaction
RNA, Bacterial/*genetics
RNA, Ribosomal, 23S/*genetics
Streptococcus/*classification/genetics
|
| Remarks: |
23S ribosomal RNa (rRNA) gene sequences of Streptococcus uberis and Strep. parauberis were determined by direct polymerase chain reaction (PCR) sequencing. Oligonucleotide probes specific for Strep. uberis and Strep. parauberis were designed from variable regions of the 23S rRNA gene sequence data. Molecular hybridizations with PCR-amplified rRNA gene targets provided a precise and reliable means of differentiating Strep. uberis and Strep. parauberis from each other and from other streptococcal species. |
| URL: |
7683638 |
|
| Ref #: |
82554 |
| Author(s): |
Chen,C.C.;Teng,L.J.;Chang,T.C. |
| Journal: |
J Clin Microbiol |
| Title: |
Identification of clinically relevant viridans group streptococci by sequence analysis of the 16S-23S ribosomal DNA spacer region |
| Volume: |
42 |
| Page(s): |
2651-7 |
| Year: |
2004 |
| Keyword(s): |
DNA, Ribosomal Spacer/*chemistry
Humans
Phylogeny
Polymerase Chain Reaction
RNA, Ribosomal, 16S/*genetics
RNA, Ribosomal, 23S/*genetics
Sequence Analysis, DNA
Viridans Streptococci/classification/genetics/*isolation & purification
|
| Remarks: |
The feasibility of sequence analysis of the 16S-23S ribosomal DNA (rDNA) intergenic spacer (ITS) for the identification of clinically relevant viridans group streptococci (VS) was evaluated. The ITS regions of 29 reference strains (11 species) of VS were amplified by PCR and sequenced. These 11 species were Streptococcus anginosus, S. constellatus, S. gordonii, S. intermedius, S. mitis, S. mutans, S. oralis, S. parasanguinis, S. salivarius, S. sanguinis, and S. uberis. The ITS lengths (246 to 391 bp) and sequences were highly conserved among strains within a species. The intraspecies similarity scores for the ITS sequences ranged from 0.98 to 1.0, except for the score for S. gordonii strains. The interspecies similarity scores for the ITS sequences varied from 0.31 to 0.93. Phylogenetic analysis of the ITS regions revealed that evolution of the regions of some species of VS is not parallel to that of the 16S rRNA genes. One hundred six clinical isolates of VS were identified by the Rapid ID 32 STREP system (bioMerieux Vitek, Marcy l'Etoile, France) and by ITS sequencing, and the level of disagreement between the two methods was 18% (19 isolates). Most isolates producing discrepant results could be unambiguously assigned to a specific species by their ITS sequences. The accuracy of using ITS sequencing for identification of VS was verified by 16S rDNA sequencing for all strains except strains of S. oralis and S. mitis, which were difficult to differentiate by their 16S rDNA sequences. In conclusion, identification of species of VS by ITS sequencing is reliable and could be used as an alternative accurate method for identification of VS. |
| URL: |
15184447 |
|
| Ref #: |
13418 |
| Author(s): |
Harland,N.M.;Leigh,J.A.;Collins,M.D. |
| Journal: |
J Appl Bacteriol |
| Title: |
Development of gene probes for the specific identification of Streptococcus uberis and Streptococcus parauberis based upon large subunit rRNA gene sequences |
| Volume: |
74 |
| Page(s): |
526-31 |
| Year: |
1993 |
| Keyword(s): |
Bacterial Typing Techniques
Base Sequence
DNA, Bacterial/genetics
DNA, Ribosomal/*genetics
Genes, Bacterial
Molecular Sequence Data
Nucleic Acid Hybridization
*Oligonucleotide Probes
Polymerase Chain Reaction
RNA, Bacterial/*genetics
RNA, Ribosomal, 23S/*genetics
Streptococcus/*classification/genetics
Support, Non-U.S. Gov't
|
| Remarks: |
23S ribosomal RNa (rRNA) gene sequences of Streptococcus uberis and Strep. parauberis were determined by direct polymerase chain reaction (PCR) sequencing. Oligonucleotide probes specific for Strep. uberis and Strep. parauberis were designed from variable regions of the 23S rRNA gene sequence data. Molecular hybridizations with PCR-amplified rRNA gene targets provided a precise and reliable means of differentiating Strep. uberis and Strep. parauberis from each other and from other streptococcal species. |
| URL: |
93252678 |
|
| Ref #: |
13427 |
| Author(s): |
Hassan,A.A.;Khan,I.U.;Abdulmawjood,A.;Lammler,C. |
| Journal: |
J Clin Microbiol |
| Title: |
Evaluation of PCR methods for rapid identification and differentiation of Streptococcus uberis and Streptococcus parauberis |
| Volume: |
39 |
| Page(s): |
1618-21 |
| Year: |
2001 |
| Keyword(s): |
GENBANK/AF255656
GENBANK/AF255657
Animal
Base Sequence
Cattle
Cattle Diseases/microbiology
DNA, Ribosomal Spacer/*genetics
*Genes, rRNA
Mastitis, Bovine/*microbiology
Molecular Sequence Data
Polymerase Chain Reaction/*methods
RNA, Ribosomal, 16S/genetics
RNA, Ribosomal, 23S/genetics
Sequence Analysis, DNA
Streptococcal Infections/microbiology/*veterinary
Streptococcus/*classification/genetics
Time Factors
|
| Remarks: |
Streptococcus uberis and Streptococcus parauberis reference strains and isolates obtained from routine diagnostics were investigated by PCR with oligonucleotide primers designed according to species-specific parts of the 16S rRNA gene, the 23S rRNA gene, and the 16S-23S rRNA intergenic spacer region of both species. All three primer pairs allowed an identification of 67 isolates as S. uberis and 4 isolates as S. parauberis. |
| URL: |
21179298 |
|
| Ref #: |
1300 |
| Author(s): |
Skerman,V.B.D.;McGowan,V.;Sneath,P.H.A.(ed) |
| Journal: |
Int. J. Syst. Bacteriol. |
| Title: |
Approved Lists of Bacterial Names. |
| Volume: |
30 |
| Page(s): |
225-420 |
| Year: |
1980 |
|
| Ref #: |
3220 |
| Author(s): |
Collins,M.D.;Farrow,J.A.E.;Katic,V.;Kandler,O. |
| Journal: |
System. Appl. Microbiol. |
| Title: |
Taxonomic studies on streptococci of serological groups E, P, U, and V: description of Streptococcus porcinus sp. nov. |
| Volume: |
5 |
| Page(s): |
402-413 |
| Year: |
1984 |
|
| Ref #: |
3345 |
| Author(s): |
Kilpper-Bälz,R.;Wenzig,P.;Schleifer,K.H. |
| Journal: |
Int. J. Syst. Bacteriol. |
| Title: |
Molecular relationships and classification of some viridans streptococci as Streptococcus oralis and emended description of Streptococcus oralis (Bridge and Sneath 1982). |
| Volume: |
35 |
| Page(s): |
482-488 |
| Year: |
1985 |
|
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