Culture Collections

Bacteria and Mycoplasmas detail

Conditions of Supply of Microbial Pathogens: Safety

Bacteria Collection: Bacillus subtilis

NCTC Number: NCTC 3610
Current Name: Bacillus subtilis
Original Strain Reference: Marburg
Other Collection No: ATCC 6051; CCM 2216; CN 5638; CONN; DSM 10; IAM 12118; IFO 12210; JCM 1465; LMG 7135; MARBURG; N R SMITH 744; NCIB 3610; NCIB 3610; NRS 744;
Previous Catalogue Name: Bacillus subtilis
Type Strain: Yes
Family: Bacillaceae
Hazard Group (ACDP): 2
Release Restrictions: Terms & Conditions of Supply of Microbial Pathogens: Safety
Conditions for growth on solid media: Nutrient agar, 24-48 hours, 37°C, aerobic
Conditions for growth on liquid media: nutrient broth,37, aerobic
16S rRNA Gene Sequence: >gb|AJ276351|DSM10|Bacillus subtilis 16S rRNA gene, strain DSM10.| cctggctcaggacga... >gb|AB042061|IAM 12118T|Bacillus subtilis gene for 16S ribosomal RNA, partial sequence.| tttatcggagagttt...
Bibliography: CONN H J 1930 J INFECT DIS 46 34;SMITH N R ET AL 1964 J GEN MICROBIOL 34
Extended Bibliography: showhide Show bibliography
Ref #: 20159
Author(s): De Clerck,E.;Vanhoutte,T.;Hebb,T.;Geerinck,J.;Devos,J.;De Vos,P.
Journal: Appl Environ Microbiol
Title: Isolation, characterization, and identification of bacterial contaminants in semifinal gelatin extracts
Volume: 70
Page(s): 3664-72
Year: 2004
Keyword(s): Animals Bacillus/*classification/genetics/*isolation & purification/physiology Cattle DNA Gyrase/genetics DNA, Ribosomal/analysis DNA-Directed RNA Polymerases/genetics Drug Industry Endospore-Forming Bacteria/*classification/genetics/*isolation & purification/physiology Food Industry *Gelatin Gelatinases/metabolism Phylogeny Polymerase Chain Reaction RNA, Ribosomal, 16S/genetics Sequence Analysis, DNA Species Specificity Spores, Bacterial/isolation & purification
Remarks: Bacterial contamination of gelatin is of great concern. Indeed, this animal colloid has many industrial applications, mainly in food and pharmaceutical products. In a previous study (E. De Clerck and P. De Vos, Syst. Appl. Microbiol. 25:611-618), contamination of a gelatin production process with a variety of gram-positive and gram-negative bacteria was demonstrated. In this study, bacterial contamination of semifinal gelatin extracts from several production plants was examined. Since these extracts are subjected to harsh conditions during production and a final ultrahigh-temperature treatment, the bacterial load at this stage is expected to be greatly reduced. In total, 1,129 isolates were obtained from a total of 73 gelatin batches originating from six different production plants. Each of these batches was suspected of having bacterial contamination based on quality control testing at the production plant from which it originated. For characterization and identification of the 1,129 bacterial isolates, repetitive-element PCR was used to obtain manageable groups. Representative strains were identified by means of 16S rRNA gene sequencing, species-specific gyrB PCR, and gyrA and rpoB sequencing and were tested for gelatinase activity. The majority of isolates belonged to members of Bacillus or related endospore-forming genera. Representative strains were identified as Bacillus cereus, Bacillus coagulans, Bacillus fumarioli, Bacillus amyloliquefaciens, Bacillus licheniformis, Bacillus pumilus, Bacillus sonorensis, Bacillus subtilis, Bacillus gelatini, Bacillus thermoamylovorans, Anoxybacillus contaminans, Anoxybacillus flavithermus, Brevibacillus agri, Brevibacillus borstelensis, and Geobacillus stearothermophilus. The majority of these species include strains exhibiting gelatinase activity. Moreover, some of these species have known pathogenic properties. These findings are of great concern with regard to the safety and quality of gelatin and its applications.
URL: 15184171
Ref #: 1300
Author(s): Skerman,V.B.D.;McGowan,V.;Sneath,P.H.A.(ed)
Journal: Int. J. Syst. Bacteriol.
Title: Approved Lists of Bacterial Names.
Volume: 30
Page(s): 225-420
Year: 1980
Ref #: 1509
Author(s): Guthrie,R.;Susi,A.
Journal: Pediatrics
Title: A simple phenylalanine method for detection of phenylketonuria in large populations of newborn infants.
Volume: 32
Page(s): 338-343
Year: 1963
Ref #: 3768
Author(s): Fahmy,F.;Flossdorf,J.;Claus,D.
Journal: System. Appl. Microbiol.
Title: The DNA base composition of the type strains of the genus Bacillus.
Volume: 6
Page(s): 60-65
Year: 1985
Ref #: 6615
Author(s): Heyndrickx,M.;Vandermeulebroecke,K.;Kersters,K.;DeVos,P.;Logan,N.A.;Aziz,M.A.;Ali,N.;Berkeley,R.C.W.
Journal: Int. J. Syst. Bacteriol.
Title: A polyphasic reassessment of the genus Paenibacillus, reclassification of Bacillus lautus (Nakamura 1984) as Paenibacillus lautus comb. nov. and of Bacillus peoriae (Montefusco et al. 1993) as Paenibacillus peoriae comb. nov., and emended description of
Volume: 46
Page(s): 988-1003
Year: 1996
Ref #: 6924
Author(s): DeutschesInstitutfürNormungDIN.NormenausschußMedizin(NAMed)
Title: DIN 58959-7. Qualitätsmanagement in der medizinischen Mikrobiologie. Teil 7: Allgemeine Anforderungen an das Mitführen von Kontrollstämmen. Beiblatt 2: ATCC- und DSM-Nummern häufig verwendeter Kontrollstämme.
Year: 1997
Ref #: 736
Author(s): Ansorg,R.;Zappel,H.;Thomssen,R.
Journal: Zentralbl. Bakteriol. Parasitenkd. Orig. Reihe A
Title: Bedeutung des Nachweises antibakterieller Stoffe im Urin für die bakteriologische Diagnostik und die Kontrolle der Chemotherapie von Harnwegsinfektionen.
Volume: 230
Page(s): 492-507
Year: 1975
Ref #: 7456
Author(s): Nakamura,L.K.;Roberts,M.S.;Cohan,F.M.
Journal: Int. J. Syst. Bacteriol.
Title: Relationship of Bacillus subtilis clades associated with strains 168 and W23: a proposal for Bacillus subtilis subsp. subtilis subsp. nov. and Bacillus subtilis subsp. spizizenii subsp. nov.
Volume: 49
Page(s): 1211-1215
Year: 1999
Data: (Conn, N. R. Smith 744, DSM 10, ATCC 6051, NCIB 3610, CN 5638) Type strain / R. S. Breed, New York in 1932 / Conn, H. J. (1930) J. infect. Dis. 46, 34 / Breed, R. S. & St. John-Brooks, R. (1935) Zentbl. Bakt. ParasitKde, II. Abt. 92, 481 / Smith, N. R. et al. (1964) J. gen. Microbiol. 34, 269
Accession Date: 01/01/1932
Authority: (Ehrenberg 1835) Cohn 1872 (AL)
Depositor: BREED R S
Taxonomy: TaxLink: S436 (Bacillus subtilis subsp. subtilis (Ehrenberg 1835) Cohn 1872) - Date of change: 5/02/2003
Biosafety Responsibility: It is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country

Additional Information

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The Culture Collections hold cell cultures, bacteria, fungi and virus strains from worldwide sources. Our scientists ensure that the identification of the cultures is correct and they remain unchanged from when they are first deposited with the Collection. Nevertheless, some of the data we provide about the cultures is supplied by the person depositing the strains and, although we have multiple checking procedures in place, we cannot always verify all their data. Please note that the Culture Collections cannot be held responsible for any inaccuracies in the data provided by the depositors.

Cultures supplied by Culture Collections are to be used as controls for microbiology testing and for research purposes only. Please view the Terms & Conditions of Supply for more information.

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