|NCTC Number:||NCTC 14385|
|Current Name:||Clostridioides difficile|
|Original Strain Reference:||IB136|
|Hazard Group (ACDP):||2|
|Release Restrictions:||Terms & Conditions of Supply of Microbial Pathogens: Safety|
|Conditions for growth on solid media:||Blood agar or fastidious anaerobe agar, 37°C, AnO₂, 24 - 48 hours|
|Isolated From:||Human with symptomatic C. difficile infection, faecal material. Leiden, The Netherlands. 03/02/2016.|
|Miscellaneous Sequence Data:||ENA Genome Accn: CAADHH010000000|
|Bibliography:||Boekhoud IM, Hornung BVH, Sevilla E, et al. Plasmid-mediated metronidazole resistance in Clostridioides difficile. Nat Commun. 2020;11(1):598. Published 2020 Jan 30.|
|Data:||Carries the 7-kb pCD-METRO plasmid / Phenotypically resistant to metronidazole / Carries an uncharacterised pCD6-family plasmid / At the request of the depositor, this strain is to be used for research purposes only, and not any commercial purpose, application or process.|
|Other Typing Information:||PCR Ribotype 020, MLST type 002|
|History:||Accessioned into the NCTC < Dr. Wiep Klass Smiths < Isolated by C�line Harmanus (Leiden University Medical Center, Leiden, The Netherlands)|
|Authority:||(Hall and O'Toole 1935) Lawson et al. 2016|
|Depositor:||Dr. Wiep Klaas Smits (Leiden University Medical Center, Dept. of Medical Microbiology, Leiden, The Netherlands)|
|Biosafety Responsibility:||It is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country|
The Culture Collections hold cell cultures, bacteria, fungi and virus strains from worldwide sources. Our scientists ensure that the identification of the cultures is correct and they remain unchanged from when they are first deposited with the Collection. Nevertheless, some of the data we provide about the cultures is supplied by the person depositing the strains and, although we have multiple checking procedures in place, we cannot always verify all their data. Please note that the Culture Collections cannot be held responsible for any inaccuracies in the data provided by the depositors.
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