Culture Collections

Bacteria and Mycoplasmas detail

Conditions of Supply of Microbial Pathogens: Safety

Bacteria Collection: Clostridioides difficile

NCTC Number: NCTC 14385
Current Name: Clostridioides difficile
Original Strain Reference: IB136
Type Strain: No
Family: Peptostreptococcaceae
Hazard Group (ACDP): 2
Release Restrictions: Terms & Conditions of Supply of Microbial Pathogens: Safety
Conditions for growth on solid media: Blood agar or fastidious anaerobe agar, 37°C, AnO₂, 24 - 48 hours
Isolated From: Human with symptomatic C. difficile infection, faecal material. Leiden, The Netherlands. 03/02/2016.
Miscellaneous Sequence Data: ENA Genome Accn: CAADHH010000000
Bibliography: Boekhoud IM, Hornung BVH, Sevilla E, et al. Plasmid-mediated metronidazole resistance in Clostridioides difficile. Nat Commun. 2020;11(1):598. Published 2020 Jan 30.
Data: Carries the 7-kb pCD-METRO plasmid / Phenotypically resistant to metronidazole / Carries an uncharacterised pCD6-family plasmid / At the request of the depositor, this strain is to be used for research purposes only, and not any commercial purpose, application or process.
Other Typing Information: PCR Ribotype 020, MLST type 002
Accession Date: 08.10.2019
History: Accessioned into the NCTC < Dr. Wiep Klass Smiths < Isolated by C�line Harmanus (Leiden University Medical Center, Leiden, The Netherlands)
Authority: (Hall and O'Toole 1935) Lawson et al. 2016
Depositor: Dr. Wiep Klaas Smits (Leiden University Medical Center, Dept. of Medical Microbiology, Leiden, The Netherlands)
Biosafety Responsibility: It is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country

Additional Information

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The Culture Collections hold cell cultures, bacteria, fungi and virus strains from worldwide sources. Our scientists ensure that the identification of the cultures is correct and they remain unchanged from when they are first deposited with the Collection. Nevertheless, some of the data we provide about the cultures is supplied by the person depositing the strains and, although we have multiple checking procedures in place, we cannot always verify all their data. Please note that the Culture Collections cannot be held responsible for any inaccuracies in the data provided by the depositors.

Cultures supplied by Culture Collections are to be used as controls for microbiology testing and for research purposes only. Please view the Terms & Conditions of Supply for more information.

Contact us if you want to discuss commercial use of the cultures.

Available Formats

Ampoule (Bacteria)

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