|NCTC Number:||NCTC 14379|
|Current Name:||Escherichia coli|
|Original Strain Reference:||MC1000 + pDM1-mcr-3|
|Hazard Group (ACDP):||2|
|Release Restrictions:||Terms & Conditions of Supply of Microbial Pathogens: Safety|
|Conditions for growth on solid media:||Nutrient agar, 37°C, O₂, 24 hours|
|Isolated From:||GMO organism derived from Escherichia coli MC1000. London, UK.|
M J Casadaban, S N Cohen. J Mol Biol. 1980 Apr;138(2):179-207.
L Dortet et al. Rapid detection and discrimination of chromosome- and MCR-plasmid-mediated resistance to polymyxins by MALDI-TOF MS in Escherichia coli: the MALDIxin test. J Antimicrob Chemother. 2018 Dec 1;73(12):3359-3367.
This organism has been genetically modified and is GMO2 / pDM1 is a tetracycline selectable synthetic plasmid containing a p15a ori that is maintained at ~10 copies per cell. mcr-3 is cloned into the multiple cloning site of this plasmid, and is IPTG-inducible and driven by a Ptac promoter.
pDM1 sequence: Genbank MN128719.
The sequence of the mcr-3 ORF is available on request.
|History:||Accessioned into the NCTC < Engineered by Despoina Mavridou|
|Authority:||(Migula 1895) Castellani and Chalmers 1919|
|Depositor:||Despoina Mavridou (Imperial College London, Department of Life Sciences, Kensington, London, UK)|
|Biosafety Responsibility:||It is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country|
The Culture Collections hold cell cultures, bacteria, fungi and virus strains from worldwide sources. Our scientists ensure that the identification of the cultures is correct and they remain unchanged from when they are first deposited with the Collection. Nevertheless, some of the data we provide about the cultures is supplied by the person depositing the strains and, although we have multiple checking procedures in place, we cannot always verify all their data. Please note that the Culture Collections cannot be held responsible for any inaccuracies in the data provided by the depositors.
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