Extended Bibliography: |
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Ref #: |
95477 |
Author(s): |
Korczak,B.;Christensen,H.;Emler,S.;Frey,J.;Kuhnert,P. |
Journal: |
Int J Syst Evol Microbiol |
Title: |
Phylogeny of the family Pasteurellaceae based on rpoB sequences |
Volume: |
54 |
Page(s): |
1393-9 |
Year: |
2004 |
Keyword(s): |
Bacterial Proteins/genetics
DNA, Bacterial/chemistry/isolation & purification
DNA, Ribosomal/chemistry
DNA-Directed RNA Polymerases/*genetics
Genes, rRNA
Molecular Sequence Data
Pasteurellaceae/*classification/*genetics
Phylogeny
RNA, Bacterial/genetics
RNA, Ribosomal, 16S/genetics
Sequence Analysis, DNA
Sequence Homology
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Remarks: |
Sequences of the gene encoding the beta-subunit of the RNA polymerase (rpoB) were used to delineate the phylogeny of the family Pasteurellaceae. A total of 72 strains, including the type strains of the major described species as well as selected field isolates, were included in the study. Selection of universal rpoB-derived primers for the family allowed straightforward amplification and sequencing of a 560 bp fragment of the rpoB gene. In parallel, 16S rDNA was sequenced from all strains. The phylogenetic tree obtained with the rpoB sequences reflected the major branches of the tree obtained with the 16S rDNA, especially at the genus level. Only a few discrepancies between the trees were observed. In certain cases the rpoB phylogeny was in better agreement with DNA-DNA hybridization studies than the phylogeny derived from 16S rDNA. The rpoB gene is strongly conserved within the various species of the family of Pasteurellaceae. Hence, rpoB gene sequence analysis in conjunction with 16S rDNA sequencing is a valuable tool for phylogenetic studies of the Pasteurellaceae and may also prove useful for reorganizing the current taxonomy of this bacterial family. |
URL: |
15280320 |
|
Ref #: |
47767 |
Author(s): |
Christensen,H.;Kuhnert,P.;Olsen,J.E.;Bisgaard,M. |
Journal: |
Int J Syst Evol Microbiol |
Title: |
Comparative phylogenies of the housekeeping genes atpD, infB and rpoB and the 16S rRNA gene within the Pasteurellaceae |
Volume: |
54 |
Page(s): |
1601-9 |
Year: |
2004 |
Keyword(s): |
GENBANK/AY296207
GENBANK/AY296208
GENBANK/AY296209
GENBANK/AY296210
GENBANK/AY296211
GENBANK/AY296212
GENBANK/AY296213
GENBANK/AY296214
GENBANK/AY296215
GENBANK/AY296216
GENBANK/AY296217
GENBANK/AY296218
GENBANK/AY296219
GENBANK/AY296220
GENBANK/AY296221
GENBANK/AY296222
GENBANK/AY296223
GENBANK/AY296224
GENBANK/AY296225
GENBANK/AY296226
GENBANK/AY296227
GENBANK/AY296228
GENBANK/AY296229
GENBANK/AY296230
GENBANK/AY296231
GENBANK/AY296232
GENBANK/AY296233
GENBANK/AY296234
GENBANK/AY296235
GENBANK/AY296236
GENBANK/AY296237
GENBANK/AY296238
GENBANK/AY296239
GENBANK/AY296240
GENBANK/AY296241
GENBANK/AY296242
GENBANK/AY296243
GENBANK/AY296244
GENBANK/AY296245
GENBANK/AY296246
GENBANK/AY314025
GENBANK/AY314043
Actinobacillus/genetics
Bacterial Proteins/genetics
DNA, Bacterial/chemistry/isolation & purification
DNA, Ribosomal/chemistry/isolation & purification
DNA-Directed RNA Polymerases/*genetics
Evolution
Genes, Bacterial
Genes, rRNA
Haemophilus/genetics
Mannheimia/genetics
Molecular Sequence Data
Pasteurella/genetics
Pasteurellaceae/*classification/*genetics
Phylogeny
Prokaryotic Initiation Factor-2/*genetics
RNA, Bacterial/genetics
RNA, Ribosomal, 16S/*genetics
Sequence Analysis, DNA
Transcription Factors/*genetics
|
Remarks: |
Phylogenies of housekeeping gene and 16S rRNA gene sequences were compared to improve the classification of the bacterial family Pasteurellaceae and knowledge of the evolutionary relationships of its members. Deduced partial protein sequences of the housekeeping genes atpD, infB and rpoB were compared in 28, 36 and 28 representative taxa of the Pasteurellaceae, respectively. The monophyly of representatives of the genus Gallibacterium was recognized by analysis of all housekeeping genes, while members of Mannheimia, Actinobacillus sensu stricto and the core group of Pasteurella sensu stricto formed monophyletic groups with two out of three housekeeping genes. Representatives of Mannheimia, Actinobacillus sensu stricto, [Haemophilus] ducreyi and [Pasteurella] trehalosi formed a monophyletic unit by analysis of all three housekeeping genes, which was in contrast to the 16S rRNA gene-derived phylogeny, where these taxa occurred at separate positions in the phylogenetic tree. Representatives of the Rodent, Avian and Aphrophilus-Haemophilus 16S rRNA gene groups were weakly supported by phylogenetic analysis of housekeeping genes. Phylogenies derived by comparison of the housekeeping genes diverged significantly from the 16S rRNA gene-derived phylogeny as evaluated by the likelihood ratio test. A low degree of congruence was also observed between the individual housekeeping gene-derived phylogenies. Estimates on speciation derived from 16S rRNA and housekeeping gene sequence comparisons resulted in quite different evolutionary scenarios for members of the Pasteurellaceae. The phylogeny based on the housekeeping genes supported observed host associations between Mannheimia, Actinobacillus sensu stricto and [Pasteurella] trehalosi and animals with paired hooves. |
URL: |
15388716 |
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