Culture Collections

Bacteria and Mycoplasmas detail

Conditions of Supply of Microbial Pathogens: Safety

Bacteria Collection: Pasteurella aerogenes

NCTC Number: NCTC 13378
Current Name: Pasteurella aerogenes
Other Collection No: ATCC 27883; DSM 10153
Type Strain: Yes
Family: Pasteurellaceae
Hazard Group (ACDP): 2
Release Restrictions: Terms & Conditions of Supply of Microbial Pathogens: Safety
Conditions for growth on solid media: Columbia blood agar, 24-48 hours, 37°C, aerobic
Whole Genome Sequence:
Extended Bibliography: showhide Show bibliography
Ref #: 95477
Author(s): Korczak,B.;Christensen,H.;Emler,S.;Frey,J.;Kuhnert,P.
Journal: Int J Syst Evol Microbiol
Title: Phylogeny of the family Pasteurellaceae based on rpoB sequences
Volume: 54
Page(s): 1393-9
Year: 2004
Keyword(s): Bacterial Proteins/genetics DNA, Bacterial/chemistry/isolation & purification DNA, Ribosomal/chemistry DNA-Directed RNA Polymerases/*genetics Genes, rRNA Molecular Sequence Data Pasteurellaceae/*classification/*genetics Phylogeny RNA, Bacterial/genetics RNA, Ribosomal, 16S/genetics Sequence Analysis, DNA Sequence Homology
Remarks: Sequences of the gene encoding the beta-subunit of the RNA polymerase (rpoB) were used to delineate the phylogeny of the family Pasteurellaceae. A total of 72 strains, including the type strains of the major described species as well as selected field isolates, were included in the study. Selection of universal rpoB-derived primers for the family allowed straightforward amplification and sequencing of a 560 bp fragment of the rpoB gene. In parallel, 16S rDNA was sequenced from all strains. The phylogenetic tree obtained with the rpoB sequences reflected the major branches of the tree obtained with the 16S rDNA, especially at the genus level. Only a few discrepancies between the trees were observed. In certain cases the rpoB phylogeny was in better agreement with DNA-DNA hybridization studies than the phylogeny derived from 16S rDNA. The rpoB gene is strongly conserved within the various species of the family of Pasteurellaceae. Hence, rpoB gene sequence analysis in conjunction with 16S rDNA sequencing is a valuable tool for phylogenetic studies of the Pasteurellaceae and may also prove useful for reorganizing the current taxonomy of this bacterial family.
URL: 15280320
Ref #: 47767
Author(s): Christensen,H.;Kuhnert,P.;Olsen,J.E.;Bisgaard,M.
Journal: Int J Syst Evol Microbiol
Title: Comparative phylogenies of the housekeeping genes atpD, infB and rpoB and the 16S rRNA gene within the Pasteurellaceae
Volume: 54
Page(s): 1601-9
Year: 2004
Keyword(s): GENBANK/AY296207 GENBANK/AY296208 GENBANK/AY296209 GENBANK/AY296210 GENBANK/AY296211 GENBANK/AY296212 GENBANK/AY296213 GENBANK/AY296214 GENBANK/AY296215 GENBANK/AY296216 GENBANK/AY296217 GENBANK/AY296218 GENBANK/AY296219 GENBANK/AY296220 GENBANK/AY296221 GENBANK/AY296222 GENBANK/AY296223 GENBANK/AY296224 GENBANK/AY296225 GENBANK/AY296226 GENBANK/AY296227 GENBANK/AY296228 GENBANK/AY296229 GENBANK/AY296230 GENBANK/AY296231 GENBANK/AY296232 GENBANK/AY296233 GENBANK/AY296234 GENBANK/AY296235 GENBANK/AY296236 GENBANK/AY296237 GENBANK/AY296238 GENBANK/AY296239 GENBANK/AY296240 GENBANK/AY296241 GENBANK/AY296242 GENBANK/AY296243 GENBANK/AY296244 GENBANK/AY296245 GENBANK/AY296246 GENBANK/AY314025 GENBANK/AY314043 Actinobacillus/genetics Bacterial Proteins/genetics DNA, Bacterial/chemistry/isolation & purification DNA, Ribosomal/chemistry/isolation & purification DNA-Directed RNA Polymerases/*genetics Evolution Genes, Bacterial Genes, rRNA Haemophilus/genetics Mannheimia/genetics Molecular Sequence Data Pasteurella/genetics Pasteurellaceae/*classification/*genetics Phylogeny Prokaryotic Initiation Factor-2/*genetics RNA, Bacterial/genetics RNA, Ribosomal, 16S/*genetics Sequence Analysis, DNA Transcription Factors/*genetics
Remarks: Phylogenies of housekeeping gene and 16S rRNA gene sequences were compared to improve the classification of the bacterial family Pasteurellaceae and knowledge of the evolutionary relationships of its members. Deduced partial protein sequences of the housekeeping genes atpD, infB and rpoB were compared in 28, 36 and 28 representative taxa of the Pasteurellaceae, respectively. The monophyly of representatives of the genus Gallibacterium was recognized by analysis of all housekeeping genes, while members of Mannheimia, Actinobacillus sensu stricto and the core group of Pasteurella sensu stricto formed monophyletic groups with two out of three housekeeping genes. Representatives of Mannheimia, Actinobacillus sensu stricto, [Haemophilus] ducreyi and [Pasteurella] trehalosi formed a monophyletic unit by analysis of all three housekeeping genes, which was in contrast to the 16S rRNA gene-derived phylogeny, where these taxa occurred at separate positions in the phylogenetic tree. Representatives of the Rodent, Avian and Aphrophilus-Haemophilus 16S rRNA gene groups were weakly supported by phylogenetic analysis of housekeeping genes. Phylogenies derived by comparison of the housekeeping genes diverged significantly from the 16S rRNA gene-derived phylogeny as evaluated by the likelihood ratio test. A low degree of congruence was also observed between the individual housekeeping gene-derived phylogenies. Estimates on speciation derived from 16S rRNA and housekeeping gene sequence comparisons resulted in quite different evolutionary scenarios for members of the Pasteurellaceae. The phylogeny based on the housekeeping genes supported observed host associations between Mannheimia, Actinobacillus sensu stricto and [Pasteurella] trehalosi and animals with paired hooves.
URL: 15388716
Accession Date: 14/06/2007
Taxonomy: TaxLink: S2215 (Pasteurella aerogenes mcallister and carter 1974 emend. christensen et al. 2005) - Date of change: 31/05/2007
Biosafety Responsibility: It is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country

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The Culture Collections hold cell cultures, bacteria, fungi and virus strains from worldwide sources. Our scientists ensure that the identification of the cultures is correct and they remain unchanged from when they are first deposited with the Collection. Nevertheless, some of the data we provide about the cultures is supplied by the person depositing the strains and, although we have multiple checking procedures in place, we cannot always verify all their data. Please note that the Culture Collections cannot be held responsible for any inaccuracies in the data provided by the depositors.

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