Extended Bibliography: |
Show bibliography
Ref #: |
32077 |
Author(s): |
Lawson,A.J.;On,S.L.;Logan,J.M.;Stanley,J. |
Journal: |
Int J Syst Evol Microbiol |
Title: |
Campylobacter hominis sp. nov., from the human gastrointestinal tract |
Volume: |
51 |
Page(s): |
651-60 |
Year: |
2001 |
Keyword(s): |
GENBANK/AJ251584
Bacterial Typing Techniques
Base Composition
Campylobacter/*classification/isolation & purification
DNA, Ribosomal/genetics
Diarrhea/microbiology
Feces/*microbiology
Humans
Intestines/*microbiology
Molecular Sequence Data
Nucleic Acid Hybridization
Phenotype
RNA, Ribosomal, 16S/genetics
Sequence Analysis, DNA
Terminology
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Remarks: |
Sequences of 16S rDNA of a novel campylobacter from faeces of healthy humans were previously shown to originate from a new taxon, 'Candidatus Campylobacter hominis', which could not be cultured. Since phylogenetic analysis suggested that anaerobic conditions might be required for growth, an isolation strategy was developed employing initial non-selective membrane filtration onto fastidious anaerobe agar. Campylobacters were then isolated from the resulting mixed microbial flora by a dilution strategy and/or by immunomagnetic separation with genus-specific polyclonal antibody. Isolates were identified by a genus and taxon-specific PCR assay, and 16S rDNA nucleotide sequence analysis was carried out. All isolates exhibited the typical Campylobacter characteristics of being non-fermentative, oxidase-positive, catalase-negative and Gram-negative. Unusually, however, they were straight rods lacking flagella. The 16S rDNA nucleotide sequence analysis, DNA and mol% G+C were consistent with a new Campylobacter species whose nearest phylogenetic neighbours were Campylobacter gracilis and Campylobacter sputorum. The unique species status of the isolates was further confirmed by taxonomic analysis of 47 phenotypic characteristics. The name Campylobacter hominis sp. nov. is proposed for the new species, the type strain of which is NCTC 13146T (= LMG 19568T). |
URL: |
11321111 |
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Ref #: |
95450 |
Author(s): |
Korczak,B.M.;Stieber,R.;Emler,S.;Burnens,A.P.;Frey,J.;Kuhnert,P. |
Journal: |
Int J Syst Evol Microbiol |
Title: |
Genetic relatedness within the genus Campylobacter inferred from rpoB sequences |
Volume: |
56 |
Page(s): |
937-45 |
Year: |
2006 |
Keyword(s): |
Bacterial Proteins/*genetics
Base Sequence
Campylobacter/*classification/*genetics
DNA Primers
DNA, Bacterial/chemistry/genetics
DNA, Ribosomal/chemistry/genetics
DNA-Directed RNA Polymerases/*genetics
Molecular Sequence Data
*Phylogeny
Polymerase Chain Reaction
RNA, Ribosomal, 16S/genetics
Sequence Analysis, DNA
Statistics
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Remarks: |
The genus Campylobacter comprises 17 species, some of which are important animal and human pathogens. To gain more insight into the genetic relatedness of this genus and to improve the molecular tools available for diagnosis, a universal sequencing approach was established for the gene encoding the beta-subunit of RNA polymerase (rpoB) for the genus Campylobacter. A total of 59 strains, including the type strains of currently recognized species as well as field isolates, were investigated in the study. A primer set specific for Campylobacter species enabled straightforward amplification and sequencing of a 530 bp fragment of the rpoB gene. The 16S rRNA gene sequences of all of the strains were determined in parallel. A good congruence was obtained between 16S rRNA and rpoB gene sequence-based trees within the genus Campylobacter. The branching of the rpoB tree was similar to that of the 16S rRNA gene tree, even though a few discrepancies were observed for certain species. The resolution of the rpoB gene within the genus Campylobacter was generally much higher than that of the 16S rRNA gene sequence, resulting in a clear separation of most species and even some subspecies. The universally applicable amplification and sequencing approach for partial rpoB gene sequence determination provides a powerful tool for DNA sequence-based discrimination of Campylobacter species. |
URL: |
16627635 |
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