Bacteria Collection: Staphylococcus aureus
|Other Collection No:
|Hazard Group (ACDP):
||Terms & Conditions of Supply of Microbial Pathogens: Safety
|Conditions for growth on solid media:
Nutrient agar, 24-48 hours, 37°C, aerobic
|Whole Genome Sequence:
|16S rRNA Gene Sequence:
>gb|AF037106|M103|Nitrosomonas europaea 16S ribosomal RNA gene, complete sequence.| agagtttgatcttgg...
||Appl Environ Microbiol
||Combined molecular and conventional analyses of nitrifying bacterium diversity in activated sludge: Nitrosococcus mobilis and Nitrospira-like bacteria as dominant populations
Bradyrhizobiaceae/genetics/*isolation & purification/metabolism
Colony Count, Microbial
Gram-Negative Chemolithotrophic Bacteria/genetics/*isolation &
In Situ Hybridization, Fluorescence
Molecular Sequence Data
Polymerase Chain Reaction
RNA, Ribosomal, 16S/genetics
Sequence Analysis, DNA
||The ammonia-oxidizing and nitrite-oxidizing bacterial populations occurring in the nitrifying activated sludge of an industrial wastewater treatment plant receiving sewage with high ammonia concentrations were studied by use of a polyphasic approach. In situ hybridization with a set of hierarchical 16S rRNA-targeted probes for ammonia-oxidizing bacteria revealed the dominance of Nitrosococcus mobilis-like bacteria. The phylogenetic affiliation suggested by fluorescent in situ hybridization (FISH) was confirmed by isolation of N. mobilis as the numerically dominant ammonia oxidizer and subsequent comparative 16S rRNA gene (rDNA) sequence and DNA-DNA hybridization analyses. For molecular fine-scale analysis of the ammonia-oxidizing population, a partial stretch of the gene encoding the active-site polypeptide of ammonia monooxygenase (amoA) was amplified from total DNA extracted from ammonia oxidizer isolates and from activated sludge. However, comparative sequence analysis of 13 amoA clone sequences from activated sludge demonstrated that these sequences were highly similar to each other and to the corresponding amoA gene fragments of Nitrosomonas europaea Nm50 and the N. mobilis isolate. The unexpected high sequence similarity between the amoA gene fragments of the N. mobilis isolate and N. europaea indicates a possible lateral gene transfer event. Although a Nitrobacter strain was isolated, members of the nitrite-oxidizing genus Nitrobacter were not detectable in the activated sludge by in situ hybridization. Therefore, we used the rRNA approach to investigate the abundance of other well-known nitrite-oxidizing bacterial genera. Three different methods were used for DNA extraction from the activated sludge. For each DNA preparation, almost full-length genes encoding small-subunit rRNA were separately amplified and used to generate three 16S rDNA libraries. By comparative sequence analysis, 2 of 60 randomly selected clones could be assigned to the nitrite-oxidizing bacteria of the genus Nitrospira. Based on these clone sequences, a specific 16S rRNA-targeted probe was developed. FISH of the activated sludge with this probe demonstrated that Nitrospira-like bacteria were present in significant numbers (9% of the total bacterial counts) and frequently occurred in coaggregated microcolonies with N. mobilis.
EMRSA type EMRSA-10
TaxLink: S2775 (Staphylococcus aureus subspecies aureus rosenbach 1884) - Date of change: 16/06/2007 by NCTCUp to 16/06/2007: S4386 (Staphylococcus aureus subspecies aureus rosenbach 1884) - Date of change: 31/05/2007
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The Culture Collections hold cell cultures, bacteria, fungi and virus strains from worldwide sources. Our scientists ensure that the identification of the cultures is correct and they remain unchanged from when they are first deposited with the Collection. Nevertheless, some of the data we provide about the cultures is supplied by the person depositing the strains and, although we have multiple checking procedures in place, we cannot always verify all their data. Please note that the Culture Collections cannot be held responsible for any inaccuracies in the data provided by the depositors.
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