Culture Collections

Bacteria and Mycoplasmas detail

Conditions of Supply of Microbial Pathogens: Safety





Bacteria Collection: Staphylococcus aureus

NCTC Number: NCTC 13137
Current Name: Staphylococcus aureus
Other Collection No: M103
Type Strain: No
Family: Staphylococcaceae
Hazard Group (ACDP): 2
Release Restrictions: Terms & Conditions of Supply of Microbial Pathogens: Safety
Conditions for growth on solid media: Nutrient agar, 24-48 hours, 37°C, aerobic
Whole Genome Sequence: http://www.ebi.ac.uk/ena/data/view/ERS798852
Annotated Genome: ftp://ftp.sanger.ac.uk/pub/project/pathogens/NCTC3000/d...
16S rRNA Gene Sequence: >gb|AF037106|M103|Nitrosomonas europaea 16S ribosomal RNA gene, complete sequence.| agagtttgatcttgg...
Extended Bibliography: showhide Show bibliography
Ref #: 96183
Author(s): Juretschko,S.;Timmermann,G.;Schmid,M.;Schleifer,K.H.;Pommerening-Roser,A.;Koops,H.P.;Wagner,M.
Journal: Appl Environ Microbiol
Title: Combined molecular and conventional analyses of nitrifying bacterium diversity in activated sludge: Nitrosococcus mobilis and Nitrospira-like bacteria as dominant populations
Volume: 64
Page(s): 3042-51
Year: 1998
Keyword(s): GENBANK/AF033558 GENBANK/AF033559 GENBANK/AF037105 GENBANK/AF037106 GENBANK/AF037107 GENBANK/AF037108 GENBANK/AF043707 GENBANK/AF043708 GENBANK/AF043709 GENBANK/AF043710 GENBANK/AF043711 GENBANK/AF043712 GENBANK/AF043713 GENBANK/AF043714 GENBANK/AF043715 GENBANK/AF043716 GENBANK/AF043717 GENBANK/AF043718 GENBANK/AF043719 GENBANK/AF096836 Ammonia/*metabolism Bradyrhizobiaceae/genetics/*isolation & purification/metabolism Colony Count, Microbial DNA, Ribosomal/analysis Genes, Bacterial Gram-Negative Chemolithotrophic Bacteria/genetics/*isolation & purification In Situ Hybridization, Fluorescence Industrial Microbiology Molecular Sequence Data Nitrites/*metabolism Oxidation-Reduction Oxidoreductases/*genetics/metabolism Phylogeny Polymerase Chain Reaction RNA, Ribosomal, 16S/genetics Sequence Analysis, DNA Sewage/*microbiology
Remarks: The ammonia-oxidizing and nitrite-oxidizing bacterial populations occurring in the nitrifying activated sludge of an industrial wastewater treatment plant receiving sewage with high ammonia concentrations were studied by use of a polyphasic approach. In situ hybridization with a set of hierarchical 16S rRNA-targeted probes for ammonia-oxidizing bacteria revealed the dominance of Nitrosococcus mobilis-like bacteria. The phylogenetic affiliation suggested by fluorescent in situ hybridization (FISH) was confirmed by isolation of N. mobilis as the numerically dominant ammonia oxidizer and subsequent comparative 16S rRNA gene (rDNA) sequence and DNA-DNA hybridization analyses. For molecular fine-scale analysis of the ammonia-oxidizing population, a partial stretch of the gene encoding the active-site polypeptide of ammonia monooxygenase (amoA) was amplified from total DNA extracted from ammonia oxidizer isolates and from activated sludge. However, comparative sequence analysis of 13 amoA clone sequences from activated sludge demonstrated that these sequences were highly similar to each other and to the corresponding amoA gene fragments of Nitrosomonas europaea Nm50 and the N. mobilis isolate. The unexpected high sequence similarity between the amoA gene fragments of the N. mobilis isolate and N. europaea indicates a possible lateral gene transfer event. Although a Nitrobacter strain was isolated, members of the nitrite-oxidizing genus Nitrobacter were not detectable in the activated sludge by in situ hybridization. Therefore, we used the rRNA approach to investigate the abundance of other well-known nitrite-oxidizing bacterial genera. Three different methods were used for DNA extraction from the activated sludge. For each DNA preparation, almost full-length genes encoding small-subunit rRNA were separately amplified and used to generate three 16S rDNA libraries. By comparative sequence analysis, 2 of 60 randomly selected clones could be assigned to the nitrite-oxidizing bacteria of the genus Nitrospira. Based on these clone sequences, a specific 16S rRNA-targeted probe was developed. FISH of the activated sludge with this probe demonstrated that Nitrospira-like bacteria were present in significant numbers (9% of the total bacterial counts) and frequently occurred in coaggregated microcolonies with N. mobilis.
URL: 9687471
Data: EMRSA type EMRSA-10
Accession Date: 14/06/2007
Taxonomy: TaxLink: S2775 (Staphylococcus aureus subspecies aureus rosenbach 1884) - Date of change: 16/06/2007 by NCTCUp to 16/06/2007: S4386 (Staphylococcus aureus subspecies aureus rosenbach 1884) - Date of change: 31/05/2007
Biosafety Responsibility: It is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country

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The Culture Collections hold cell cultures, bacteria, fungi and virus strains from worldwide sources. Our scientists ensure that the identification of the cultures is correct and they remain unchanged from when they are first deposited with the Collection. Nevertheless, some of the data we provide about the cultures is supplied by the person depositing the strains and, although we have multiple checking procedures in place, we cannot always verify all their data. Please note that the Culture Collections cannot be held responsible for any inaccuracies in the data provided by the depositors.

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