Culture Collections

Bacteria and Mycoplasmas detail

Conditions of Supply of Microbial Pathogens: Safety

Bacteria Collection: Bacillus mycoides

NCTC Number: NCTC 12974
Current Name: Bacillus mycoides
Other Collection No: ATCC 6462; CIP 103472; DSM 2048
Type Strain: Yes
Family: Bacillaceae
Hazard Group (ACDP): 2
Release Restrictions: Terms & Conditions of Supply of Microbial Pathogens: Safety
16S rRNA Gene Sequence: >gb|AJ841874|DSM 2048|Bacillus mycoides partial 16S rRNA gene and ITS1, strain DSM 2048.| cattaagttgggcac... >gb|EF210295|ATCC 6462|Bacillus mycoides strain ATCC 6462 16S ribosomal RNA gene, partialsequence.| tacatgcaagtcgag...
Extended Bibliography: showhide Show bibliography
Ref #: 95473
Author(s): Nubel,U.;Schmidt,P.M.;Reiss,E.;Bier,F.;Beyer,W.;Naumann,D.
Journal: FEMS Microbiol Lett
Title: Oligonucleotide microarray for identification of Bacillus anthracis based on intergenic transcribed spacers in ribosomal DNA
Volume: 240
Page(s): 215-23
Year: 2004
Keyword(s): GENBANK/AJ841817 GENBANK/AJ841818 GENBANK/AJ841819 GENBANK/AJ841820 GENBANK/AJ841821 GENBANK/AJ841822 GENBANK/AJ841823 GENBANK/AJ841824 GENBANK/AJ841825 GENBANK/AJ841826 GENBANK/AJ841827 GENBANK/AJ841828 GENBANK/AJ841829 GENBANK/AJ841830 GENBANK/AJ841831 GENBANK/AJ841832 GENBANK/AJ841833 GENBANK/AJ841834 GENBANK/AJ841835 GENBANK/AJ841836 GENBANK/AJ841837 GENBANK/AJ841838 GENBANK/AJ841839 GENBANK/AJ841840 GENBANK/AJ841841 GENBANK/AJ841842 GENBANK/AJ841843 GENBANK/AJ841844 GENBANK/AJ841845 GENBANK/AJ841846 GENBANK/AJ841847 GENBANK/AJ841848 GENBANK/AJ841849 GENBANK/AJ841850 GENBANK/AJ841851 GENBANK/AJ841852 GENBANK/AJ841853 GENBANK/AJ841854 GENBANK/AJ841855 GENBANK/AJ841856 GENBANK/AJ841857 GENBANK/AJ841858 GENBANK/AJ841859 GENBANK/AJ841860 GENBANK/AJ841861 GENBANK/AJ841862 GENBANK/AJ841863 GENBANK/AJ841864 GENBANK/AJ841865 GENBANK/AJ841866 GENBANK/AJ841867 GENBANK/AJ841868 GENBANK/AJ841869 GENBANK/AJ841870 GENBANK/AJ841871 GENBANK/AJ841872 GENBANK/AJ841873 GENBANK/AJ841874 GENBANK/AJ841875 GENBANK/AJ841876 GENBANK/AJ841877 GENBANK/AJ841878 GENBANK/AJ841879 GENBANK/AJ841880 GENBANK/AJ841881 GENBANK/AJ841882 GENBANK/AJ841883 GENBANK/AJ841884 GENBANK/AJ841885 GENBANK/AJ841886 GENBANK/AJ841887 GENBANK/AJ841888 GENBANK/AJ841889 GENBANK/AJ841890 GENBANK/AJ841891 GENBANK/AJ841892 GENBANK/AJ841893 GENBANK/AJ841894 GENBANK/AJ841895 Bacillus anthracis/*classification/genetics/*isolation & purification Base Pair Mismatch DNA, Bacterial/chemistry/genetics DNA, Ribosomal Spacer/*genetics Molecular Sequence Data Nucleic Acid Hybridization *Oligonucleotide Array Sequence Analysis Phylogeny RNA, Bacterial/genetics RNA, Transfer, Ile/genetics Sensitivity and Specificity Sequence Analysis, DNA Sequence Homology, Nucleic Acid
Remarks: We developed a DNA microarray for identification of Bacillus anthracis and other phylogenetic groupings within the "Bacillus cereus group". Nucleotide sequences of 16S-23S ribosomal DNA internal transcribed spacers containing genes for tRNA(Ile) from 52 B. anthracis strains were found to be identical to sequences from seven strains published previously and different from all other bacteria. When 42 oligonucleotide probes targeting polymorphic sites were immobilized on glass slides and hybridized to fluorescently labeled PCR amplification products, one or more mismatches could be discriminated in all but one cases. Hence, hybridization events were highly specific and identification of B. anthracis was straightforward.
URL: 15522510
Accession Date: 14/06/2007
Taxonomy: TaxLink: S420 (Bacillus mycoides flügge 1886) - Date of change: 31/05/2007
Biosafety Responsibility: It is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country

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The Culture Collections hold cell cultures, bacteria, fungi and virus strains from worldwide sources. Our scientists ensure that the identification of the cultures is correct and they remain unchanged from when they are first deposited with the Collection. Nevertheless, some of the data we provide about the cultures is supplied by the person depositing the strains and, although we have multiple checking procedures in place, we cannot always verify all their data. Please note that the Culture Collections cannot be held responsible for any inaccuracies in the data provided by the depositors.

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Ampoule (Bacteria)

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