Bacteria Collection: Bacillus mycoides
|Other Collection No:
ATCC 6462; CIP 103472; DSM 2048
|Hazard Group (ACDP):
||Terms & Conditions of Supply of Microbial Pathogens: Safety
|16S rRNA Gene Sequence:
>gb|AJ841874|DSM 2048|Bacillus mycoides partial 16S rRNA gene and ITS1, strain DSM 2048.|
>gb|EF210295|ATCC 6462|Bacillus mycoides strain ATCC 6462 16S ribosomal RNA gene, partialsequence.|
||FEMS Microbiol Lett
||Oligonucleotide microarray for identification of Bacillus anthracis based on intergenic transcribed spacers in ribosomal DNA
Bacillus anthracis/*classification/genetics/*isolation & purification
Base Pair Mismatch
DNA, Ribosomal Spacer/*genetics
Molecular Sequence Data
Nucleic Acid Hybridization
*Oligonucleotide Array Sequence Analysis
RNA, Transfer, Ile/genetics
Sensitivity and Specificity
Sequence Analysis, DNA
Sequence Homology, Nucleic Acid
||We developed a DNA microarray for identification of Bacillus anthracis and other phylogenetic groupings within the "Bacillus cereus group". Nucleotide sequences of 16S-23S ribosomal DNA internal transcribed spacers containing genes for tRNA(Ile) from 52 B. anthracis strains were found to be identical to sequences from seven strains published previously and different from all other bacteria. When 42 oligonucleotide probes targeting polymorphic sites were immobilized on glass slides and hybridized to fluorescently labeled PCR amplification products, one or more mismatches could be discriminated in all but one cases. Hence, hybridization events were highly specific and identification of B. anthracis was straightforward.
TaxLink: S420 (Bacillus mycoides flügge 1886) - Date of change: 31/05/2007
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The Culture Collections hold cell cultures, bacteria, fungi and virus strains from worldwide sources. Our scientists ensure that the identification of the cultures is correct and they remain unchanged from when they are first deposited with the Collection. Nevertheless, some of the data we provide about the cultures is supplied by the person depositing the strains and, although we have multiple checking procedures in place, we cannot always verify all their data. Please note that the Culture Collections cannot be held responsible for any inaccuracies in the data provided by the depositors.
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