Bacteria Collection: Pseudomonas aeruginosa
|Other Collection No:
ATCC 27853; DSM 1117; WDCM 00025
|Hazard Group (ACDP):
||Terms & Conditions of Supply of Microbial Pathogens: Safety
|Conditions for growth on solid media:
Nutrient agar, 24-48 hours, 37°C, aerobic
|Whole Genome Sequence:
|16S rRNA Gene Sequence:
>gb|AY268175|ATCC 27853|Pseudomonas aeruginosa strain ATCC 27853 16S ribosomal RNA gene,partial sequence.| agattgaacgctggc...
||J Clin Microbiol
||Use of 16S rRNA gene sequencing for rapid identification and differentiation of Burkholderia pseudomallei and B. mallei
*Bacterial Typing Techniques
Molecular Sequence Data
RNA, Ribosomal, 16S/*genetics
*Sequence Analysis, DNA
||Burkholderia pseudomallei and B. mallei, the causative agents of melioidosis and glanders, respectively, are designated category B biothreat agents. Current methods for identifying these organisms rely on their phenotypic characteristics and an extensive set of biochemical reactions. We evaluated the use of 16S rRNA gene sequencing to rapidly identify these two species and differentiate them from each other as well as from closely related species and genera such as Pandoraea spp., Ralstonia spp., Burkholderia gladioli, Burkholderia cepacia, Burkholderia thailandensis, and Pseudomonas aeruginosa. We sequenced the 1.5-kb 16S rRNA gene of 56 B. pseudomallei and 23 B. mallei isolates selected to represent a wide range of temporal, geographic, and origin diversity. Among all 79 isolates, a total of 11 16S types were found based on eight positions of difference. Nine 16S types were identified in B. pseudomallei isolates based on six positions of difference, with differences ranging from 0.5 to 1.5 bp. Twenty-two of 23 B. mallei isolates showed 16S rRNA gene sequence identity and were designated 16S type 10, whereas the remaining isolate was designated type 11. This report provides a basis for rapidly identifying and differentiating B. pseudomallei and B. mallei by molecular methods.
TaxLink: S2402 (Pseudomonas aeruginosa (schroeter 1872) migula 1900) - Date of change: 31/05/2007
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