Culture Collections

Bacteria and Mycoplasmas detail

Conditions of Supply of Microbial Pathogens: Safety

Bacteria Collection: Pseudomonas aeruginosa

NCTC Number: NCTC 12903
Current Name: Pseudomonas aeruginosa
Other Collection No: ATCC 27853; WDCM 00025
Type Strain: No
Family: Pseudomonadaceae
Hazard Group (ACDP): 2
Release Restrictions: Terms & Conditions of Supply of Microbial Pathogens: Safety
Conditions for growth on solid media: Nutrient agar, 24-48 hours, 37°C, aerobic
Whole Genome Sequence:
Annotated Genome:
16S rRNA Gene Sequence: >gb|AY268175|ATCC 27853|Pseudomonas aeruginosa strain ATCC 27853 16S ribosomal RNA gene,partial sequence.| agattgaacgctggc...
Miscellaneous Sequence Data: >gb|EF064840|ATCC 27853|Pseudomonas aeruginosa strain ATCC 27853 gyrase B subunit (gyrB)gene, partial cds.| tacaaggtgtccggc...
Extended Bibliography: showhide Show bibliography
Ref #: 31040
Author(s): Gee,J.E.;Sacchi,C.T.;Glass,M.B.;De,B.K.;Weyant,R.S.;Levett,P.N.;Whitney,A.M.;Hoffmaster,A.R.;Popovic,T.
Journal: J Clin Microbiol
Title: Use of 16S rRNA gene sequencing for rapid identification and differentiation of Burkholderia pseudomallei and B. mallei
Volume: 41
Page(s): 4647-54
Year: 2003
Keyword(s): GDB/AY268140 GENBANK/AY268141 GENBANK/AY268142 GENBANK/AY268143 GENBANK/AY268144 GENBANK/AY268145 GENBANK/AY268146 GENBANK/AY268147 GENBANK/AY268148 GENBANK/AY268149 GENBANK/AY268150 GENBANK/AY268151 GENBANK/AY268152 GENBANK/AY268153 GENBANK/AY268154 GENBANK/AY268155 GENBANK/AY268156 GENBANK/AY268157 GENBANK/AY268158 GENBANK/AY268159 GENBANK/AY268160 GENBANK/AY268161 GENBANK/AY268162 GENBANK/AY268163 GENBANK/AY268164 GENBANK/AY268165 GENBANK/AY268166 GENBANK/AY268167 GENBANK/AY268168 GENBANK/AY268169 GENBANK/AY268170 GENBANK/AY268171 GENBANK/AY268172 GENBANK/AY268173 GENBANK/AY268174 GENBANK/AY268175 GENBANK/AY268176 GENBANK/AY268177 GENBANK/AY268178 GENBANK/AY268179 GENBANK/AY268180 GENBANK/AY268181 GENBANK/AY305738 GENBANK/AY305739 GENBANK/AY305740 GENBANK/AY305741 GENBANK/AY305742 GENBANK/AY305743 GENBANK/AY305744 GENBANK/AY305745 GENBANK/AY305746 GENBANK/AY305747 GENBANK/AY305748 GENBANK/AY305749 GENBANK/AY305750 GENBANK/AY305751 GENBANK/AY305752 GENBANK/AY305753 GENBANK/AY305754 GENBANK/AY305755 GENBANK/AY305756 GENBANK/AY305757 GENBANK/AY305758 GENBANK/AY305759 GENBANK/AY305760 GENBANK/AY305763 GENBANK/AY305764 GENBANK/AY305765 GENBANK/AY305766 GENBANK/AY305767 GENBANK/AY305768 GENBANK/AY305769 GENBANK/AY305770 GENBANK/AY305771 GENBANK/AY305772 GENBANK/AY305773 GENBANK/AY305774 GENBANK/AY305775 GENBANK/AY305776 GENBANK/AY305777 GENBANK/AY305778 GENBANK/AY305779 GENBANK/AY305780 GENBANK/AY305781 GENBANK/AY305782 GENBANK/AY305783 GENBANK/AY305784 GENBANK/AY305785 GENBANK/AY305786 GENBANK/AY305787 GENBANK/AY305788 GENBANK/AY305789 GENBANK/AY305790 GENBANK/AY305791 GENBANK/AY305792 GENBANK/AY305793 GENBANK/AY305794 GENBANK/AY305795 GENBANK/AY305796 GENBANK/AY305797 GENBANK/AY305798 GENBANK/AY305799 GENBANK/AY305800 GENBANK/AY305801 GENBANK/AY305802 GENBANK/AY305803 GENBANK/AY305804 GENBANK/AY305805 GENBANK/AY305806 GENBANK/AY305807 GENBANK/AY305808 GENBANK/AY305809 GENBANK/AY305810 GENBANK/AY305811 GENBANK/AY305812 GENBANK/AY305813 GENBANK/AY305814 GENBANK/AY305815 GENBANK/AY305816 GENBANK/AY305817 GENBANK/AY305818 Animals *Bacterial Typing Techniques Burkholderia/*classification/genetics Burkholderia Infections/microbiology Burkholderia pseudomallei/*classification/genetics DNA, Ribosomal/analysis Environmental Microbiology *Genes, rRNA Humans Melioidosis/microbiology Molecular Sequence Data RNA, Ribosomal, 16S/*genetics *Sequence Analysis, DNA
Remarks: Burkholderia pseudomallei and B. mallei, the causative agents of melioidosis and glanders, respectively, are designated category B biothreat agents. Current methods for identifying these organisms rely on their phenotypic characteristics and an extensive set of biochemical reactions. We evaluated the use of 16S rRNA gene sequencing to rapidly identify these two species and differentiate them from each other as well as from closely related species and genera such as Pandoraea spp., Ralstonia spp., Burkholderia gladioli, Burkholderia cepacia, Burkholderia thailandensis, and Pseudomonas aeruginosa. We sequenced the 1.5-kb 16S rRNA gene of 56 B. pseudomallei and 23 B. mallei isolates selected to represent a wide range of temporal, geographic, and origin diversity. Among all 79 isolates, a total of 11 16S types were found based on eight positions of difference. Nine 16S types were identified in B. pseudomallei isolates based on six positions of difference, with differences ranging from 0.5 to 1.5 bp. Twenty-two of 23 B. mallei isolates showed 16S rRNA gene sequence identity and were designated 16S type 10, whereas the remaining isolate was designated type 11. This report provides a basis for rapidly identifying and differentiating B. pseudomallei and B. mallei by molecular methods.
URL: 14532197
Accession Date: 14/06/2007
Taxonomy: TaxLink: S2402 (Pseudomonas aeruginosa (schroeter 1872) migula 1900) - Date of change: 31/05/2007
Biosafety Responsibility: It is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country

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The Culture Collections hold cell cultures, bacteria, fungi and virus strains from worldwide sources. Our scientists ensure that the identification of the cultures is correct and they remain unchanged from when they are first deposited with the Collection. Nevertheless, some of the data we provide about the cultures is supplied by the person depositing the strains and, although we have multiple checking procedures in place, we cannot always verify all their data. Please note that the Culture Collections cannot be held responsible for any inaccuracies in the data provided by the depositors.

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