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Bacteria Collection: Porphyromonas cangingivalis

NCTC Number: NCTC 12856
Current Name: Porphyromonas cangingivalis
Other Collection No: VPB4874=AHN4309
Type Strain: Yes
Family: Porphyromonadaceae
Hazard Group (ACDP): 2
Release Restrictions: Terms & Conditions of Supply of Microbial Pathogens: Safety
16S rRNA Gene Sequence: >gb|AY546479|NCTC 12856|Porphyromonas cangingivalis strain NCTC 12856 16S-23S ribosomal RNAintergenic spacer, partial sequence.| cgcaggatcgcacga...
23S rRNA Gene Sequence: >gb|AY546479|NCTC 12856|Porphyromonas cangingivalis strain NCTC 12856 16S-23S ribosomal RNAintergenic spacer, partial sequence.| cgcaggatcgcacga...
Extended Bibliography: showhide Show bibliography
Ref #: 25447
Author(s): Conrads,G.;Citron,D.M.;Tyrrell,K.L.;Horz,H.P.;Goldstein,E.J.
Journal: Int J Syst Evol Microbiol
Title: 16S-23S rRNA gene internal transcribed spacer sequences for analysis of the phylogenetic relationships among species of the genus Porphyromonas
Volume: 55
Page(s): 607-13
Year: 2005
Keyword(s): GENBANK/AB034799 GENBANK/AB035460 GENBANK/AF208290 GENBANK/AY546472 GENBANK/AY546473 GENBANK/AY546474 GENBANK/AY546475 GENBANK/AY546476 GENBANK/AY546477 GENBANK/AY546478 GENBANK/AY546479 GENBANK/AY546480 GENBANK/AY546481 GENBANK/AY546482 GENBANK/AY546483 GENBANK/AY546484 GENBANK/AY546485 GENBANK/AY546486 GENBANK/AY546487 GENBANK/AY546488 GENBANK/AY546489 GENBANK/AY546490 Bacterial Typing Techniques DNA, Ribosomal Spacer/*analysis Genes, rRNA Humans Molecular Sequence Data *Phylogeny Porphyromonas/*classification/genetics RNA, Ribosomal, 16S/*genetics RNA, Ribosomal, 23S/*genetics Sequence Analysis, DNA Species Specificity
Remarks: The 16S-23S rRNA gene internal transcribed spacer (ITS) regions of 11 reference strains of Porphyromonas species, together with Bacteroides distasonis and Tannerella forsythensis, were analysed to examine interspecies relationships. Compared with the phylogenetic tree generated using 16S rRNA gene sequences, the resolution of the ITS sequence-based tree was higher, but species positioning and clustering were similar with both approaches. The recent separation of Porphyromonas gulae and Porphyromonas gingivalis into distinct species was confirmed by the ITS data. In addition, analysis of the ITS sequences of 24 clinical isolates of Porphyromonas asaccharolytica plus the type strain ATCC 25260(T) divided the sequences into two clusters, of which one was alpha-fucosidase-positive (like the type strain) while the other was alpha-fucosidase-negative. The latter resembled the previously studied unusual extra-oral isolates of 'Porphyromonas endodontalis-like organisms' (PELOs) which could therefore be called 'Porphyromonas asaccharolytica-like organisms' (PALOs), based on the genetic identification. Moreover, the proposal of alpha-fucosidase-negative P. asaccharolytica strains as a new species should also be considered.
URL: 15774632
Ref #: 69143
Author(s): Morse,R.;O'Hanlon,K.;Collins,M.D.
Journal: Int J Syst Evol Microbiol
Title: Phylogenetic, amino acid content and indel analyses of the beta subunit of DNA-dependent RNA polymerase of gram-positive and gram-negative bacteria
Volume: 52
Page(s): 1477-84
Year: 2002
Keyword(s): GENBANK/AE001944 GENBANK/Y16466 GENBANK/Y16467 GENBANK/Y16468 GENBANK/Y16469 GENBANK/Y16470 GENBANK/Y16471 GENBANK/Y16472 GENBANK/Y19223 Amino Acid Sequence Amino Acids/analysis Base Composition Base Sequence DNA Transposable Elements DNA, Bacterial/chemistry/genetics DNA-Directed RNA Polymerases/*chemistry/*genetics Drug Resistance, Bacterial/genetics Genes, Bacterial Gram-Negative Bacteria/classification/*enzymology/*genetics Gram-Positive Bacteria/classification/*enzymology/*genetics Molecular Sequence Data Phylogeny Protein Subunits RNA, Bacterial/genetics RNA, Ribosomal, 16S/genetics Rifampin/pharmacology Sequence Deletion Sequence Homology, Amino Acid
Remarks: In this study, we have sequenced the rpoB gene, encoding the beta subunit of DNA-dependent RNA polymerase, from a selection of gram-positive and gram-negative bacteria. The presence of insertions and deletions (indels) in the beta subunit separate the gram-positive and gram-negative bacteria from each other and support the division of the gram-positive organisms into two clades based on DNA G+C content. Phylogenetic and amino acid content analyses further separate the clostridia from bacilli, leuconostocs, listeriae and relatives, forming an early branch after the common gram-positive ancestor. The occurrence in the beta subunit of Asn-Ala at positions 471-472 in Porphyromonas cangingivalis and Asn at position 372 in Weissella paramesenteroides are postulated to be the cause of the natural rifampicin resistance of these species.
URL: 12361249
Accession Date: 14/06/2007
Taxonomy: TaxLink: S4852 (Porphyromonas cangingivalis collins et al. 1994) - Date of change: 31/05/2007
Biosafety Responsibility: It is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country

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