Culture Collections

Bacteria and Mycoplasmas detail

Conditions of Supply of Microbial Pathogens: Safety





Bacteria Collection: Streptococcus cristatus

NCTC Number: NCTC 12479
Current Name: Streptococcus cristatus
Other Collection No: ATCC 51100; CCUG 33481; CIP 105954; DSM 8249; LMG 16320
Type Strain: Yes
Family: Streptococcaceae
Hazard Group (ACDP): 2
Release Restrictions: Terms & Conditions of Supply of Microbial Pathogens: Safety
Conditions for growth on solid media: Columbia blood agar, 24-48 hours, 37°C, aerobic
Whole Genome Sequence: http://www.ebi.ac.uk/ena/data/view/ERS956186
Annotated Genome: ftp://ftp.sanger.ac.uk/pub/project/pathogens/NCTC3000/...
16S rRNA Gene Sequence: >gb|AY584476|ATCC 51100|Streptococcus cristatus strain ATCC 51100 16S ribosomal RNA gene,partial sequence.| gacgaacgctggcgg...
Extended Bibliography: showhide Show bibliography
Ref #: 43548
Author(s): Picard,F.J.;Ke,D.;Boudreau,D.K.;Boissinot,M.;Huletsky,A.;Richard,D.;Ouellette,M.;Roy,P.H.;Bergeron,M.G.
Journal: J Clin Microbiol
Title: Use of tuf sequences for genus-specific PCR detection and phylogenetic analysis of 28 streptococcal species
Volume: 42
Page(s): 3686-95
Year: 2004
Keyword(s): DNA Primers Genes, Bacterial/*genetics Gram-Negative Bacteria/classification/genetics/isolation & purification Gram-Positive Bacteria/classification/genetics/isolation & purification Humans Peptide Elongation Factor Tu/*genetics Phylogeny Polymerase Chain Reaction/*methods Streptococcus/classification/genetics/*isolation & purification
Remarks: A 761-bp portion of the tuf gene (encoding the elongation factor Tu) from 28 clinically relevant streptococcal species was obtained by sequencing amplicons generated using broad-range PCR primers. These tuf sequences were used to select Streptococcus-specific PCR primers and to perform phylogenetic analysis. The specificity of the PCR assay was verified using 102 different bacterial species, including the 28 streptococcal species. Genomic DNA purified from all streptococcal species was efficiently detected, whereas there was no amplification with DNA from 72 of the 74 nonstreptococcal bacterial species tested. There was cross-amplification with DNAs from Enterococcus durans and Lactococcus lactis. However, the 15 to 31% nucleotide sequence divergence in the 761-bp tuf portion of these two species compared to any streptococcal tuf sequence provides ample sequence divergence to allow the development of internal probes specific to streptococci. The Streptococcus-specific assay was highly sensitive for all 28 streptococcal species tested (i.e., detection limit of 1 to 10 genome copies per PCR). The tuf sequence data was also used to perform extensive phylogenetic analysis, which was generally in agreement with phylogeny determined on the basis of 16S rRNA gene data. However, the tuf gene provided a better discrimination at the streptococcal species level that should be particularly useful for the identification of very closely related species. In conclusion, tuf appears more suitable than the 16S ribosomal RNA gene for the development of diagnostic assays for the detection and identification of streptococcal species because of its higher level of species-specific genetic divergence.
URL: 15297518
Ref #: 95509
Author(s): Majewski,J.;Zawadzki,P.;Pickerill,P.;Cohan,F.M.;Dowson,C.G.
Journal: J Bacteriol
Title: Barriers to genetic exchange between bacterial species: Streptococcus pneumoniae transformation
Volume: 182
Page(s): 1016-23
Year: 2000
Keyword(s): GENBANK/AF194507 GENBANK/AF194508 GENBANK/AF194509 GENBANK/AF194510 GENBANK/AF194511 GENBANK/AF194512 GENBANK/AF194513 GENBANK/AF194514 GENBANK/AF194515 GENBANK/AF194516 GENBANK/AF194517 GENBANK/AF194518 GENBANK/AF194519 GENBANK/AF194520 GENBANK/AF194521 GENBANK/AF194522 GENBANK/AF194523 GENBANK/AF194524 GENBANK/AF194525 GENBANK/AF194526 GENBANK/AF194527 GENBANK/AF194528 Base Pair Mismatch/*genetics DNA Repair/*genetics DNA, Bacterial/genetics DNA-Directed RNA Polymerases/genetics Molecular Sequence Data Phylogeny Recombination, Genetic Sequence Analysis, DNA Streptococcus/genetics Streptococcus pneumoniae/*genetics/growth & development *Transformation, Bacterial
Remarks: Interspecies genetic exchange is an important evolutionary mechanism in bacteria. It allows rapid acquisition of novel functions by transmission of adaptive genes between related species. However, the frequency of homologous recombination between bacterial species decreases sharply with the extent of DNA sequence divergence between the donor and the recipient. In Bacillus and Escherichia, this sexual isolation has been shown to be an exponential function of sequence divergence. Here we demonstrate that sexual isolation in transformation between Streptococcus pneumoniae recipient strains and donor DNA from related strains and species follows the described exponential relationship. We show that the Hex mismatch repair system poses a significant barrier to recombination over the entire range of sequence divergence (0.6 to 27%) investigated. Although mismatch repair becomes partially saturated, it is responsible for 34% of the observed sexual isolation. This is greater than the role of mismatch repair in Bacillus but less than that in Escherichia. The remaining non-Hex-mediated barrier to recombination can be provided by a variety of mechanisms. We discuss the possible additional mechanisms of sexual isolation, in view of earlier findings from Bacillus, Escherichia, and Streptococcus.
URL: 10648528
Accession Date: 14/06/2007
Taxonomy: TaxLink: S4221 (Streptococcus cristatus handley et al. 1991) - Date of change: 31/05/2007
Biosafety Responsibility: It is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country

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