Culture Collections

Bacteria and Mycoplasmas detail

Conditions of Supply of Microbial Pathogens: Safety

Bacteria Collection: Escherichia coli

NCTC Number: NCTC 11954
Current Name: Escherichia coli
Original Strain Reference: Beecham 1532
Other Collection No: ATCC 35218; BEECHAM 1532
Previous Catalogue Name: Escherichia coli
Type Strain: No
Family: Enterobacteriaceae
Hazard Group (ACDP): 2
Release Restrictions: Terms & Conditions of Supply of Microbial Pathogens: Safety
Conditions for growth on solid media: nutrient agar,37, facultative anaerobe
Conditions for growth on liquid media: nutrient agar,37, facultative anaerobe
16S rRNA Gene Sequence: >gb|EF436579|ATCC 35218|Escherichia coli 16S-23S ribosomal RNA intergenic spacer, partialsequence.| caattttcgtgtccc...
23S rRNA Gene Sequence: >gb|EF436579|ATCC 35218|Escherichia coli 16S-23S ribosomal RNA intergenic spacer, partialsequence.| caattttcgtgtccc...
Miscellaneous Sequence Data: >gb|AY370845|ATCC 35218|Escherichia coli strain ATCC 35218 gyrase B (gyrB) gene, partialcds.| aaagtgtccggcggt...
Bibliography: FUCHS P C ET AL 1983 ANTIMICROB AG CHEMO- THER 24 31
Extended Bibliography: showhide Show bibliography
Ref #: 43181
Author(s): Delmas,J.;Breysse,F.;Devulder,G.;Flandrois,J.P.;Chomarat,M.
Journal: Diagn Microbiol Infect Dis
Title: Rapid identification of Enterobacteriaceae by sequencing DNA gyrase subunit B encoding gene
Volume: 55
Page(s): 263-8
Year: 2006
Keyword(s): Bacterial Typing Techniques/*methods DNA Gyrase/*genetics Enterobacteriaceae/enzymology/genetics/*isolation & purification Genotype Humans Sequence Analysis, DNA/methods
Remarks: Real-time polymerase chain reaction and sequencing were used to characterize a 506-bp-long DNA fragment internal to the gyrB gene (gyrBint). The sequences obtained from 32 Enterobacteriaceae-type strains and those available in the Genbank nucleotide sequence database (n = 24) were used as a database to identify 240 clinical enterobacteria isolates. Sequence analysis of the gyrBint fragment of 240 strains showed that gyrBint constitutes a discriminative target sequence to differentiate between Enterobacteriaceae species. Comparison of these identifications with those obtained by phenotypic methods (Vitek 1 system and/or Rapid ID 32E; bioMerieux, Marcy l'Etoile, France) revealed discrepancies essentially with genera Citrobacter and Enterobacter. Most of the strains identified as Enterobacter cloacae by phenotypic methods were identified as Enterobacter hormaechei strains by gyrBint sequencing. The direct sequencing of gyrBint would be useful as a complementary tool in the identification of clinical Enterobacteriaceae isolates.
URL: 16626902
Data: (ATCC 35218) R. Sutherland, Beechams Pharmaceuticals, Betchworth, Surrey, 1986 / F. S. Forr, Bristol, Tennessee, 1982 / Canine / -lactamase-producing control strain for sensitivity tests with amoxycillin & clavulanic acid (Augmentin) and ticarcillin & clavulanic acid (Timetin) / Recommended control strain for serial dilution (MIC) tests and MIC breakpoint tests (R. Sutherland, pers. comm. ) / Fuchs, P. C. et al. (1983) Antimicrob. Ag. Chemother. 24, 31 / NCCLS (1984) Approved Standard M2-A3 / NCCLS (1985) Approved Standard M7-A
Accession Date: 01/01/1986
Authority: (Migula 1895) Castellani and Chalmers 1919 (AL)
Taxonomy: TaxLink: S1192 (Escherichia coli (Migula 1895) Castellani and Chalmers 1919) - Date of change: 5/02/2003
Other: ANTIBIOTIC (AND OTHER ANTIMICROBIALS) ASSAY, SENSITIVITY, RESISTANCE CONTROL STRAINSAmoxycillin / Clavulanic acid (Augmentin)B-lactamase positiveTicarcillin / Clavulanic acid (Timetin)B-lactamase positive
Biosafety Responsibility: It is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country

Additional Information

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The Culture Collections hold cell cultures, bacteria, fungi and virus strains from worldwide sources. Our scientists ensure that the identification of the cultures is correct and they remain unchanged from when they are first deposited with the Collection. Nevertheless, some of the data we provide about the cultures is supplied by the person depositing the strains and, although we have multiple checking procedures in place, we cannot always verify all their data. Please note that the Culture Collections cannot be held responsible for any inaccuracies in the data provided by the depositors.

Cultures supplied by Culture Collections are to be used as controls for microbiology testing and for research purposes only. Please view the Terms & Conditions of Supply for more information.

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Available Formats

Ampoule (Bacteria)
Bacterial DNA - 2µg

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