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Bacteria Collection: Campylobacter jejuni subsp. doylei

NCTC Number: NCTC 11951
Current Name: Campylobacter jejuni subsp. doylei
Original Strain Reference: 093, IMVS 1141
Other Collection No: ATCC 49349; CCUG 24567; LMG 8803
Previous Catalogue Name: Campylobacter jejuni subsp. doylei
Type Strain: Yes
Family: Campylobacteraceae
Hazard Group (ACDP): 2
Release Restrictions: Terms & Conditions of Supply of Microbial Pathogens: Safety
Conditions for growth on solid media: Columbia blood agar, 48 hours, 37°C, microaerophilic
Whole Genome Sequence: http://www.ebi.ac.uk/ena/data/view/ERS1273882
Extended Bibliography: showhide Show bibliography
Ref #: 32263
Author(s): Christensen,H.;Jorgensen,K.;Olsen,J.E.
Journal: Microbiology
Title: Differentiation of Campylobacter coli and C. jejuni by length and DNA sequence of the 16S-23S rRNA internal spacer region
Volume: 145 ( Pt 1)
Page(s): 99-105
Year: 1999
Keyword(s): GENBANK/AF074828 GENBANK/AF074829 GENBANK/AF074830 GENBANK/AF074831 GENBANK/AF074832 GENBANK/AF074833 GENBANK/AF074834 GENBANK/AF074835 GENBANK/AF074836 GENBANK/AF074837 GENBANK/AF074838 GENBANK/AF074839 GENBANK/AF074840 GENBANK/AF074841 Animals Bacterial Typing Techniques Base Sequence Campylobacter coli/classification/*genetics Campylobacter jejuni/classification/*genetics Chickens/microbiology Conserved Sequence/genetics DNA, Bacterial/chemistry/genetics DNA, Ribosomal/chemistry/*genetics Electrophoresis, Polyacrylamide Gel Genes, rRNA Genotype Humans Molecular Sequence Data Molecular Weight Nucleic Acid Conformation Polymerase Chain Reaction RNA, Ribosomal, 16S/*genetics RNA, Ribosomal, 23S/*genetics Swine/microbiology Variation (Genetics)/genetics
Remarks: The internal spacer region (ISR) between the 16S and 23S rRNA genes of Campylobacter was investigated by PCR fragment length typing and DNA sequencing of clinical and chicken wild-type isolates. PCR fragment length typing showed one fragment of 859 nt in length for the 12 strains of Campylobacter coli investigated. Thirty-six of the Campylobacter jejuni subsp. jejuni strains possessed one fragment, which varied in size between 727 and 802 nt. Three strains showed two fragments between 501 and 923 nt. Strains of C. jejuni subsp. doylei, Campylobacter lari and Campylobacter upsaliensis possessed one or two fragments with lengths different from those of C. coli and C. jejuni subsp. jejuni. DNA sequences were obtained from 54 nt downstream of rrs up to rrl of four strains of C. coli, eight strains of C. jejuni subsp. jejuni, and one strain each of C. jejuni subsp. doylei and C. lari, selected to represent the different biotypes of Campylobacter. ISR lengths determined by PCR fragment length typing and DNA sequencing corresponded for 12 strains. For two strains of C. coli, PCR fragment length typing underestimated ISR lengths by 159 and 193 nt, probably related to incomplete resolution of the distal helical structures, which were not fully denatured during PAGE. For the 14 strains and the published C. jejuni subsp. jejuni sequence, the first 206-211 nt were conserved and included the two tRNA genes in the characteristic tRNA(Ala) to tRNA(Ile) order separated by a short 8-9 nt spacer region. Within the region downstream of tRNA(Ile) conserved regions were identified which allowed a separation of C. lari from C. coli and C. jejuni but not separation of C. coli from C. jejuni. The 69-282 nt longer variable regions in C. coli strains allowed separation of this species from C. jejuni, confirming results obtained by PCR typing. Certain nucleic acid positions in variable regions were related to the Lior biotypes. Sequence information from ISRs of more strains is needed to ascertain if separation of species and biotypes will be possible for diagnostic purposes.
URL: 10206715
Ref #: 12362
Author(s): Christensen,H.;Jorgensen,K.;Olsen,J.E.
Journal: Microbiology
Title: Differentiation of Campylobacter coli and C. jejuni by length and DNA sequence of the 16S-23S rRNA internal spacer region
Volume: 145 ( Pt 1)
Page(s): 99-105
Year: 1999
Keyword(s): GENBANK/AF074828 GENBANK/AF074829 GENBANK/AF074830 GENBANK/AF074831 GENBANK/AF074832 GENBANK/AF074833 GENBANK/AF074834 GENBANK/AF074835 GENBANK/AF074836 GENBANK/AF074837 GENBANK/AF074838 GENBANK/AF074839 GENBANK/AF074840 GENBANK/AF074841 Animal Bacterial Typing Techniques Base Sequence Campylobacter coli/classification/*genetics Campylobacter jejuni/classification/*genetics Chickens/microbiology Comparative Study Conserved Sequence/genetics DNA, Bacterial/chemistry/genetics DNA, Ribosomal/chemistry/*genetics Electrophoresis, Polyacrylamide Gel Genes, rRNA Genotype Human Molecular Sequence Data Molecular Weight Nucleic Acid Conformation Polymerase Chain Reaction RNA, Ribosomal, 16S/*genetics RNA, Ribosomal, 23S/*genetics Swine/microbiology Variation (Genetics)/genetics
Remarks: The internal spacer region (ISR) between the 16S and 23S rRNA genes of Campylobacter was investigated by PCR fragment length typing and DNA sequencing of clinical and chicken wild-type isolates. PCR fragment length typing showed one fragment of 859 nt in length for the 12 strains of Campylobacter coli investigated. Thirty-six of the Campylobacter jejuni subsp. jejuni strains possessed one fragment, which varied in size between 727 and 802 nt. Three strains showed two fragments between 501 and 923 nt. Strains of C. jejuni subsp. doylei, Campylobacter lari and Campylobacter upsaliensis possessed one or two fragments with lengths different from those of C. coli and C. jejuni subsp. jejuni. DNA sequences were obtained from 54 nt downstream of rrs up to rrl of four strains of C. coli, eight strains of C. jejuni subsp. jejuni, and one strain each of C. jejuni subsp. doylei and C. lari, selected to represent the different biotypes of Campylobacter. ISR lengths determined by PCR fragment length typing and DNA sequencing corresponded for 12 strains. For two strains of C. coli, PCR fragment length typing underestimated ISR lengths by 159 and 193 nt, probably related to incomplete resolution of the distal helical structures, which were not fully denatured during PAGE. For the 14 strains and the published C. jejuni subsp. jejuni sequence, the first 206-211 nt were conserved and included the two tRNA genes in the characteristic tRNA(Ala) to tRNA(Ile) order separated by a short 8-9 nt spacer region. Within the region downstream of tRNA(Ile) conserved regions were identified which allowed a separation of C. lari from C. coli and C. jejuni but not separation of C. coli from C. jejuni. The 69-282 nt longer variable regions in C. coli strains allowed separation of this species from C. jejuni, confirming results obtained by PCR typing. Certain nucleic acid positions in variable regions were related to the Lior biotypes. Sequence information from ISRs of more strains is needed to ascertain if separation of species and biotypes will be possible for diagnostic purposes.
URL: 99140141
Data: (ATCC 49349, CCUG 24567) Type strain / T. W. Steele, Institute of Medical and Veterinary Science, Adelaide, South Australia in 1986 / Faeces (fluid), 2 year old child / Steele, T. W. and Owen, R. J. (1988) Int. J. syst. Bact. 38, 316
Accession Date: 04/02/2003
Authority: Steele and Owen 1988
Taxonomy: TaxLink: S674 (Campylobacter jejuni subspecies doylei steele and owen 1988) - Date of change: 16/06/2007 by NCTCUp to 16/06/2007: ? (NCTC 11951) - Date of change: 04/02/2003
Biosafety Responsibility: It is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country

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