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Bacteria Collection: Corynebacterium jeikeium

NCTC Number: NCTC 11913
Current Name: Corynebacterium jeikeium
Original Strain Reference: A376/84
Other Collection No: ATCC 43734; A376/84; CIP 103337; DSM 46361; DSM 7171; IFO 15298; IMET 11513
Previous Catalogue Name: Corynebacterium jeikeium
Type Strain: Yes
Family: Corynebacteriaceae
Hazard Group (ACDP): 2
Release Restrictions: Terms & Conditions of Supply of Microbial Pathogens: Safety
Conditions for growth on solid media: Columbia blood agar, 48 hours, 37°C, aerobic
Conditions for growth on liquid media: nutrient broth,37, aerobic
Isolated From: blood
Whole Genome Sequence: http://www.ebi.ac.uk/ena/data/view/ERS1188813
16S rRNA Gene Sequence: >gb|X84250|NCTC 11913|C.jeikeium 16S rRNA gene.| tactggctcaggacg... >gb|X82062|CIP 103337T|C.jeikeium 16S ribosomal DNA.| acgaacgctggcggc... >gb|U87823|ATCC 43734|Corynebacterium jeikeium 16S ribosomal RNA gene, complete sequence.| agagtttgacctggc... >gb|X84250|NCTC 11913|Corynebacterium jeikeium 16S rRNA gene, strain NCTC 11913.| tactggctcaggacg...
Bibliography: RILEY P S ET AL 1979 J CLIN MICRO 9 418; JACKMAN P J H ET AL 1986 J APPL
Extended Bibliography: showhide Show bibliography
Ref #: 95490
Author(s): Ruimy,R.;Riegel,P.;Boiron,P.;Monteil,H.;Christen,R.
Journal: Int J Syst Bacteriol
Title: Phylogeny of the genus Corynebacterium deduced from analyses of small-subunit ribosomal DNA sequences
Volume: 45
Page(s): 740-6
Year: 1995
Keyword(s): GENBANK/X81904 GENBANK/X81905 GENBANK/X81906 GENBANK/X81907 GENBANK/X81908 GENBANK/X81909 GENBANK/X81910 GENBANK/X81912 GENBANK/X81913 GENBANK/X81914 GENBANK/X81915 GENBANK/X81916 GENBANK/X81917 GENBANK/X81918 GENBANK/X81919 GENBANK/X81920 GENBANK/X81921 GENBANK/X81922 GENBANK/X81923 GENBANK/X81924 GENBANK/X81925 GENBANK/X81926 GENBANK/X81927 GENBANK/X81928 GENBANK/X81929 GENBANK/X81930 GENBANK/X81931 GENBANK/X81932 GENBANK/X81933 GENBANK/X81934 Base Sequence Corynebacterium/+ACo-classification/genetics DNA, Bacterial/+ACo-chemistry DNA, Ribosomal/+ACo-chemistry Molecular Sequence Data Phylogeny
Remarks: We determined almost complete small-subunit ribosomal DNA sequences of 50 reference strains belonging to the genera Corynebacterium, Rhodococcus, and Gordona and compared these sequences with previously published sequences. Three phylogenetic methods (the neighbor-joining, maximum-likelihood, and maximum-parsimony methods), as well as a bootstrap analysis, were used to assess the robustness of each topology which we obtained. The results of comparative phylogenetic analyses confirmed that the genera Corynebacterium, Dietzia, Gordona, Mycobacterium, Nocardia, Tsukamurella, and Turicella form a monophyletic taxon within the phylum containing the high-Gcontent gram-positive bacteria. The genus Corynebacterium appeared to be a monophyletic unit whose members could be divided into four major clusters. The validity of the genus Turicella is doubtful since members of this genus clearly belong to the genus Corynebacterium. The variability of chemotaxonomic characteristics within the genus Corynebacterium suggests that small-subunit ribosomal DNA sequence analysis is probably the most straightforward method for confirming that a bacterium belongs to this genus.
URL: 7547293
Ref #: 38920
Author(s): Pascual,C.;Lawson,P.A.;Farrow,J.A.;Gimenez,M.N.;Collins,M.D.
Journal: Int J Syst Bacteriol
Title: Phylogenetic analysis of the genus Corynebacterium based on 16S rRNA gene sequences
Volume: 45
Page(s): 724-8
Year: 1995
Keyword(s): GENBANK/X81871 GENBANK/X81872 GENBANK/X81874 GENBANK/X84240 GENBANK/X84241 GENBANK/X84244 GENBANK/X84245 GENBANK/X84246 GENBANK/X84247 GENBANK/X84248 GENBANK/X84249 GENBANK/X84250 GENBANK/X84251 GENBANK/X84252 GENBANK/X84253 GENBANK/X84255 GENBANK/X84256 GENBANK/X84257 GENBANK/X84258 GENBANK/X84437 GENBANK/X84438 GENBANK/X84439 GENBANK/X84440 GENBANK/X84441 GENBANK/X84442 GENBANK/X84443 GENBANK/X84444 GENBANK/X84446 GENBANK/X84678 GENBANK/X84679 Base Sequence Corynebacterium/*classification/genetics DNA, Ribosomal/*chemistry Molecular Sequence Data Phylogeny RNA, Ribosomal, 16S/*genetics
Remarks: The 16S rRNA gene sequences of 30 strains representing 23 validated Corynebacterium species and 7 currently non-valid Corynebacterium species were determined. These sequences were aligned with the sequences of other Corynebacterium species and related actinomycete taxa. A comparative sequence analysis revealed that there is considerable phylogenetic depth and internal structure in the genus Corynebacterium. Turicella otitidis and the amycolate species Corynebacterium amycolatum were located at the periphery of the genus Corynebacterium. It was evident that the species of the genus Corynebacterium form a monophyletic association and, together with other chemotype IV and mycolic acid-containing taxa (including the genera Dietzia, Gordona, Mycobacterium, Nocardia, Rhodococcus, and Tsukamurella), form a natural suprageneric group.
URL: 7547291
Ref #: 95474
Author(s): Khamis,A.;Raoult,D.;La Scola,B.
Journal: J Clin Microbiol
Title: rpoB gene sequencing for identification of Corynebacterium species
Volume: 42
Page(s): 3925-31
Year: 2004
Keyword(s): Base Sequence Corynebacterium/*classification/*genetics/isolation & purification DNA Primers DNA, Ribosomal/genetics DNA-Directed RNA Polymerases/*genetics Humans Phylogeny RNA, Ribosomal, 16S/genetics
Remarks: The genus Corynebacterium is a heterogeneous group of species comprising human and animal pathogens and environmental bacteria. It is defined on the basis of several phenotypic characters and the results of DNA-DNA relatedness and, more recently, 16S rRNA gene sequencing. However, the 16S rRNA gene is not polymorphic enough to ensure reliable phylogenetic studies and needs to be completely sequenced for accurate identification. The almost complete rpoB sequences of 56 Corynebacterium species were determined by both PCR and genome walking methods. In all cases the percent similarities between different species were lower than those observed by 16S rRNA gene sequencing, even for those species with degrees of high similarity. Several clusters supported by high bootstrap values were identified. In order to propose a method for strain identification which does not require sequencing of the complete rpoB sequence (approximately 3,500 bp), we identified an area with a high degree of polymorphism, bordered by conserved sequences that can be used as universal primers for PCR amplification and sequencing. The sequence of this fragment (434 to 452 bp) allows accurate species identification and may be used in the future for routine sequence-based identification of Corynebacterium species.
URL: 15364970
Ref #: 12499
Author(s): Ruimy,R.;Riegel,P.;Boiron,P.;Monteil,H.;Christen,R.
Journal: Int J Syst Bacteriol
Title: Phylogeny of the genus Corynebacterium deduced from analyses of small-subunit ribosomal DNA sequences
Volume: 45
Page(s): 740-6
Year: 1995
Keyword(s): GENBANK/X81904 GENBANK/X81905 GENBANK/X81906 GENBANK/X81907 GENBANK/X81908 GENBANK/X81909 GENBANK/X81910 GENBANK/X81912 GENBANK/X81913 GENBANK/X81914 GENBANK/X81915 GENBANK/X81916 GENBANK/X81917 GENBANK/X81918 GENBANK/X81919 GENBANK/X81920 GENBANK/X81921 GENBANK/X81922 GENBANK/X81923 GENBANK/X81924 GENBANK/X81925 GENBANK/X81926 GENBANK/X81927 GENBANK/X81928 GENBANK/X81929 GENBANK/X81930 GENBANK/X81931 GENBANK/X81932 GENBANK/X81933 GENBANK/X81934 Base Sequence Corynebacterium/+ACo-classification/genetics DNA, Bacterial/+ACo-chemistry DNA, Ribosomal/+ACo-chemistry Molecular Sequence Data Phylogeny
Remarks: We determined almost complete small-subunit ribosomal DNA sequences of 50 reference strains belonging to the genera Corynebacterium, Rhodococcus, and Gordona and compared these sequences with previously published sequences. Three phylogenetic methods (the neighbor-joining, maximum-likelihood, and maximum-parsimony methods), as well as a bootstrap analysis, were used to assess the robustness of each topology which we obtained. The results of comparative phylogenetic analyses confirmed that the genera Corynebacterium, Dietzia, Gordona, Mycobacterium, Nocardia, Tsukamurella, and Turicella form a monophyletic taxon within the phylum containing the high-Gcontent gram-positive bacteria. The genus Corynebacterium appeared to be a monophyletic unit whose members could be divided into four major clusters. The validity of the genus Turicella is doubtful since members of this genus clearly belong to the genus Corynebacterium. The variability of chemotaxonomic characteristics within the genus Corynebacterium suggests that small-subunit ribosomal DNA sequence analysis is probably the most straightforward method for confirming that a bacterium belongs to this genus.
URL: 96016727
Ref #: 12500
Author(s): Pascual,C.;Lawson,P.A.;Farrow,J.A.;Gimenez,M.N.;Collins,M.D.
Journal: Int J Syst Bacteriol
Title: Phylogenetic analysis of the genus Corynebacterium based on 16S rRNA gene sequences
Volume: 45
Page(s): 724-8
Year: 1995
Keyword(s): GENBANK/X81871 GENBANK/X81872 GENBANK/X81874 GENBANK/X84240 GENBANK/X84241 GENBANK/X84244 GENBANK/X84245 GENBANK/X84246 GENBANK/X84247 GENBANK/X84248 GENBANK/X84249 GENBANK/X84250 GENBANK/X84251 GENBANK/X84252 GENBANK/X84253 GENBANK/X84255 GENBANK/X84256 GENBANK/X84257 GENBANK/X84258 GENBANK/X84437 GENBANK/X84438 GENBANK/X84439 GENBANK/X84440 GENBANK/X84441 GENBANK/X84442 GENBANK/X84443 GENBANK/X84444 GENBANK/X84446 GENBANK/X84678 GENBANK/X84679 Base Sequence Corynebacterium/*classification/genetics DNA, Ribosomal/*chemistry Molecular Sequence Data Phylogeny RNA, Ribosomal, 16S/*genetics Support, Non-U.S. Gov't
Remarks: The 16S rRNA gene sequences of 30 strains representing 23 validated Corynebacterium species and 7 currently non-valid Corynebacterium species were determined. These sequences were aligned with the sequences of other Corynebacterium species and related actinomycete taxa. A comparative sequence analysis revealed that there is considerable phylogenetic depth and internal structure in the genus Corynebacterium. Turicella otitidis and the amycolate species Corynebacterium amycolatum were located at the periphery of the genus Corynebacterium. It was evident that the species of the genus Corynebacterium form a monophyletic association and, together with other chemotype IV and mycolic acid-containing taxa (including the genera Dietzia, Gordona, Mycobacterium, Nocardia, Rhodococcus, and Tsukamurella), form a natural suprageneric group.
URL: 96016725
Ref #: 3022
Journal: Int. J. Syst. Bacteriol.
Title: Validation of the publication of new names and new combinations previously effectively published outside the IJSB. List No. 24.
Volume: 38
Page(s): 136-137
Year: 1988
Ref #: 4617
Author(s): Jackman,P.J.H.;Pitcher,D.G.;Pelczynska,S.;Borman,P.
Journal: System. Appl. Microbiol.
Title: Classification of corynebacteria associated with endocarditis (group JK) as Corynebacterium jeikeium sp. nov.
Volume: 9
Page(s): 83-90
Year: 1987
Data: Type strain / D. Pitcher, NCTC in 1985 / R. E. Weaver CDC Atlanta USA / Blood / Riley, P. S. et al. (1979) J. clin. Micro. 9, 418 / Jackman, P. J. H. et al. (1987) Syst. Appl. Microbiol. 9, 83 / Validation List No. 24 Int. J. syst. Bact. 38, 136
Accession Date: 01/01/1985
History: WEAVER R E CDC ATLANTA - PITCHER D NCTC - PROPOSED TYPE STRN PRE:FR
Authority: Jackman et al. 1988
Depositor: PITCHER D
Taxonomy: TaxLink: S960 (Corynebacterium jeikeium Jackman et al. 1988) - Date of change: 5/02/2003
Biosafety Responsibility: It is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country

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The Culture Collections hold cell cultures, bacteria, fungi and virus strains from worldwide sources. Our scientists ensure that the identification of the cultures is correct and they remain unchanged from when they are first deposited with the Collection. Nevertheless, some of the data we provide about the cultures is supplied by the person depositing the strains and, although we have multiple checking procedures in place, we cannot always verify all their data. Please note that the Culture Collections cannot be held responsible for any inaccuracies in the data provided by the depositors.

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