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Bacteria Collection: Corynebacterium xerosis

NCTC Number: NCTC 11861
Current Name: Corynebacterium xerosis
Original Strain Reference: 11861
Other Collection No: ATCC 373
Previous Catalogue Name: Corynebacterium xerosis
Type Strain: Yes
Family: Corynebacteriaceae
Hazard Group (ACDP): 2
Release Restrictions: Terms & Conditions of Supply of Microbial Pathogens: Safety
Conditions for growth on solid media: Fastidious anaerobe agar, 24-48 hours, 37°C, anaerobic
Conditions for growth on liquid media: nutrient broth,37, facultative anaerobe
Isolated From: human, ear discharge of child 1924
Whole Genome Sequence: http://www.ebi.ac.uk/ena/data/view/ERS1275650
16S rRNA Gene Sequence: >gb|X81914|ATCC 373T|C.xerosis 16S rRNA gene (ATCC 373T).| acgaacgctggcggc... >gb|U02895|ATCC 373|Corynebacterium xerosis ATCC 373 16S rRNA gene, partial sequence.| atacgtcaaatcatc...
Extended Bibliography: showhide Show bibliography
Ref #: 19216
Author(s): Greisen,K.;Loeffelholz,M.;Purohit,A.;Leong,D.
Journal: J Clin Microbiol
Title: PCR primers and probes for the 16S rRNA gene of most species of pathogenic bacteria, including bacteria found in cerebrospinal fluid
Volume: 32
Page(s): 335-51
Year: 1994
Keyword(s): GENBANK/U02893 GENBANK/U02894 GENBANK/U02895 GENBANK/U02896 GENBANK/U02897 GENBANK/U02898 GENBANK/U02899 GENBANK/U02900 GENBANK/U02901 GENBANK/U02902 GENBANK/U02903 GENBANK/U02904 GENBANK/U02905 GENBANK/U02906 GENBANK/U02907 GENBANK/U02908 GENBANK/U02909 GENBANK/U02910 GENBANK/U02911 GENBANK/U02912 GENBANK/U02913 GENBANK/U02914 GENBANK/U02915 GENBANK/U02916 GENBANK/U02917 GENBANK/U02918 GENBANK/U02919 GENBANK/U02920 GENBANK/U02921 GENBANK/U02922 Bacteremia/diagnosis/microbiology Bacteria/*genetics/isolation & purification/pathogenicity Bacterial Infections/diagnosis/microbiology Base Sequence Cerebrospinal Fluid/microbiology DNA Primers/genetics DNA Probes/genetics Genes, Bacterial Gram-Negative Bacteria/genetics Gram-Positive Bacteria/genetics Humans Meningitis, Bacterial/diagnosis/microbiology Molecular Sequence Data Nucleic Acid Hybridization *Polymerase Chain Reaction/statistics & numerical data RNA, Bacterial/*genetics RNA, Ribosomal, 16S/*genetics Sensitivity and Specificity Sequence Homology, Nucleic Acid Species Specificity
Remarks: A set of broad-range PCR primers for the 16S rRNA gene in bacteria were tested, along with three series of oligonucleotide probes to detect the PCR product. The first series of probes is broad in range and consists of a universal bacterial probe, a gram-positive probe, a Bacteroides-Flavobacterium probe, and two probes for other gram-negative species. The second series was designed to detect PCR products from seven major bacterial species or groups frequently causing meningitis: Neisseria meningitidis, Haemophilus influenzae, Streptococcus pneumoniae, S. agalactiae, Escherichia coli and other enteric bacteria, Listeria monocytogenes, and Staphylococcus aureus. The third series was designed for the detection of DNA from species or genera commonly considered potential contaminants of clinical samples, including cerebrospinal fluid (CSF): Bacillus, Corynebacterium, Propionibacterium, and coagulase-negative Staphylococcus spp. The primers amplified DNA from all 124 different species of bacteria tested. Southern hybridization testing of the broad-range probes with washes containing 3 M tetramethylammonium chloride indicated that this set of probes correctly identified all but two of the 102 bacterial species tested, the exceptions being Deinococcus radiopugnans and Gardnerella vaginalis. The gram-negative and gram-positive probes hybridized to isolates of two newly characterized bacteria, Alloiococcus otitis and Rochalimaea henselii, as predicted by Gram stain characteristics. The CSF pathogen and contaminant probe sequences were compared with available sequence information and with sequencing data for 32 different species. Testing of the CSF pathogen and contaminant probes against DNA from over 60 different strains indicated that, with the exception of the coagulase-negative Staphylococcus probes, these probes provided the correct identification of bacterial species known to be found in CSF.
URL: 7512093
Ref #: 95490
Author(s): Ruimy,R.;Riegel,P.;Boiron,P.;Monteil,H.;Christen,R.
Journal: Int J Syst Bacteriol
Title: Phylogeny of the genus Corynebacterium deduced from analyses of small-subunit ribosomal DNA sequences
Volume: 45
Page(s): 740-6
Year: 1995
Keyword(s): GENBANK/X81904 GENBANK/X81905 GENBANK/X81906 GENBANK/X81907 GENBANK/X81908 GENBANK/X81909 GENBANK/X81910 GENBANK/X81912 GENBANK/X81913 GENBANK/X81914 GENBANK/X81915 GENBANK/X81916 GENBANK/X81917 GENBANK/X81918 GENBANK/X81919 GENBANK/X81920 GENBANK/X81921 GENBANK/X81922 GENBANK/X81923 GENBANK/X81924 GENBANK/X81925 GENBANK/X81926 GENBANK/X81927 GENBANK/X81928 GENBANK/X81929 GENBANK/X81930 GENBANK/X81931 GENBANK/X81932 GENBANK/X81933 GENBANK/X81934 Base Sequence Corynebacterium/+ACo-classification/genetics DNA, Bacterial/+ACo-chemistry DNA, Ribosomal/+ACo-chemistry Molecular Sequence Data Phylogeny
Remarks: We determined almost complete small-subunit ribosomal DNA sequences of 50 reference strains belonging to the genera Corynebacterium, Rhodococcus, and Gordona and compared these sequences with previously published sequences. Three phylogenetic methods (the neighbor-joining, maximum-likelihood, and maximum-parsimony methods), as well as a bootstrap analysis, were used to assess the robustness of each topology which we obtained. The results of comparative phylogenetic analyses confirmed that the genera Corynebacterium, Dietzia, Gordona, Mycobacterium, Nocardia, Tsukamurella, and Turicella form a monophyletic taxon within the phylum containing the high-Gcontent gram-positive bacteria. The genus Corynebacterium appeared to be a monophyletic unit whose members could be divided into four major clusters. The validity of the genus Turicella is doubtful since members of this genus clearly belong to the genus Corynebacterium. The variability of chemotaxonomic characteristics within the genus Corynebacterium suggests that small-subunit ribosomal DNA sequence analysis is probably the most straightforward method for confirming that a bacterium belongs to this genus.
URL: 7547293
Ref #: 13687
Author(s): Greisen,K.;Loeffelholz,M.;Purohit,A.;Leong,D.
Journal: J Clin Microbiol
Title: PCR primers and probes for the 16S rRNA gene of most species of pathogenic
Volume: 32
Page(s): 335-351
Year: 1994
Keyword(s): 0 (DNA Primers) 0 (DNA Probes) 0 (RNA, Bacterial) 0 (RNA, Ribosomal, 16S) Bacteremia/diagnosis/microbiology Bacteria/*genetics/isolation & purification/pathogenicity Bacterial Infections/diagnosis/microbiology Base Sequence Cerebrospinal Fluid/microbiology DNA Primers/genetics DNA Probes/genetics Genes, Bacterial Gram-Negative Bacteria/genetics Gram-Positive Bacteria/genetics Human Meningitis, Bacterial/diagnosis/microbiology Molecular Sequence Data Nucleic Acid Hybridization *Polymerase Chain Reaction/statistics & numerical data RNA, Bacterial/*genetics RNA, Ribosomal, 16S/*genetics Sensitivity and Specificity Sequence Homology, Nucleic Acid Species Specificity
Remarks: A set of broad-range PCR primers for the 16S rRNA gene in bacteria were
URL: 94201356
Ref #: 12499
Author(s): Ruimy,R.;Riegel,P.;Boiron,P.;Monteil,H.;Christen,R.
Journal: Int J Syst Bacteriol
Title: Phylogeny of the genus Corynebacterium deduced from analyses of small-subunit ribosomal DNA sequences
Volume: 45
Page(s): 740-6
Year: 1995
Keyword(s): GENBANK/X81904 GENBANK/X81905 GENBANK/X81906 GENBANK/X81907 GENBANK/X81908 GENBANK/X81909 GENBANK/X81910 GENBANK/X81912 GENBANK/X81913 GENBANK/X81914 GENBANK/X81915 GENBANK/X81916 GENBANK/X81917 GENBANK/X81918 GENBANK/X81919 GENBANK/X81920 GENBANK/X81921 GENBANK/X81922 GENBANK/X81923 GENBANK/X81924 GENBANK/X81925 GENBANK/X81926 GENBANK/X81927 GENBANK/X81928 GENBANK/X81929 GENBANK/X81930 GENBANK/X81931 GENBANK/X81932 GENBANK/X81933 GENBANK/X81934 Base Sequence Corynebacterium/+ACo-classification/genetics DNA, Bacterial/+ACo-chemistry DNA, Ribosomal/+ACo-chemistry Molecular Sequence Data Phylogeny
Remarks: We determined almost complete small-subunit ribosomal DNA sequences of 50 reference strains belonging to the genera Corynebacterium, Rhodococcus, and Gordona and compared these sequences with previously published sequences. Three phylogenetic methods (the neighbor-joining, maximum-likelihood, and maximum-parsimony methods), as well as a bootstrap analysis, were used to assess the robustness of each topology which we obtained. The results of comparative phylogenetic analyses confirmed that the genera Corynebacterium, Dietzia, Gordona, Mycobacterium, Nocardia, Tsukamurella, and Turicella form a monophyletic taxon within the phylum containing the high-Gcontent gram-positive bacteria. The genus Corynebacterium appeared to be a monophyletic unit whose members could be divided into four major clusters. The validity of the genus Turicella is doubtful since members of this genus clearly belong to the genus Corynebacterium. The variability of chemotaxonomic characteristics within the genus Corynebacterium suggests that small-subunit ribosomal DNA sequence analysis is probably the most straightforward method for confirming that a bacterium belongs to this genus.
URL: 96016727
Data: (ATCC 373) Type strain / ATCC in 1984 / J. C. Fitzgerald / Ear discharge of child, 1924
Accession Date: 01/01/1984
History: FITZGERALD J C - ATCC
Authority: Lehmann and Neumann 1899 (AL)
Depositor: ATCC
Taxonomy: TaxLink: S982 (Corynebacterium xerosis Lehmann and Neumann 1899) - Date of change: 5/02/2003
Biosafety Responsibility: It is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country

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