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Bacteria Collection: Porphyromonas gingivalis

NCTC Number: NCTC 11834
Current Name: Porphyromonas gingivalis
Original Strain Reference: Slots 2561
Other Collection No: ATCC 33277; SLOTS 2561
Previous Catalogue Name: Bacteroides gingivalis
Type Strain: Yes
Family: Porphyromonadaceae
Hazard Group (ACDP): 2
Release Restrictions: Terms & Conditions of Supply of Microbial Pathogens: Safety
Conditions for growth on solid media: Fastidious anaerobe agar, 72 hours, 37°C, anaerobic
Isolated From: human, human gingival sulcus
16S rRNA Gene Sequence: >gb|AF414809|ATCC 33277|Porphyromonas gingivalis strain ATCC 33277 16S ribosomal RNA gene,partial sequence.| gatgaacgctagcga... >gb|AF285870|ATCC33277|Porphyromonas gingivalis 16S ribosomal RNA gene, partial sequence.| cgctagcgataggct... >gb|AF118635|ATCC 33277|Porphyromonas gingivalis isolate ATCC 33277 16S-23S intergenicspacer region, complete sequence.| gcccgaaaaattaag... >gb|AB035459|ATCC33277|Porphyromonas gingivalis gene for 16S rRNA, partial sequence,strain:ATCC33277.| atggagagtttgatc... >gb|AY546475|ATCC 33277|Porphyromonas gingivalis strain ATCC 33277 16S-23S ribosomal RNAintergenic spacer, partial sequence.| cgcaaggggcgcact...
23S rRNA Gene Sequence: >gb|AF118635|ATCC 33277|Porphyromonas gingivalis isolate ATCC 33277 16S-23S intergenicspacer region, complete sequence.| gcccgaaaaattaag... >gb|AY546475|ATCC 33277|Porphyromonas gingivalis strain ATCC 33277 16S-23S ribosomal RNAintergenic spacer, partial sequence.| cgcaaggggcgcact...
Miscellaneous Sequence Data: >gb|AB048190|ATCC 33277|Porphyromonas gingivalis gyrB gene for DNA gyrase B subunit,partial cds.| gacaagggttcatac...
Bibliography: COYKENDALL A L ET AL 1980 INTJ SYST BACT 30 559
Extended Bibliography: showhide Show bibliography
Ref #: 95510
Author(s): Rumpf,R.W.;Griffen,A.L.;Wen,B.G.;Leys,E.J.
Journal: J Clin Microbiol
Title: Sequencing of the ribosomal intergenic spacer region for strain identification of Porphyromonas gingivalis
Volume: 37
Page(s): 2723-5
Year: 1999
Keyword(s): GENBANK/AF118633 GENBANK/AF118634 GENBANK/AF118635 *Genes, Bacterial *Genes, rRNA Polymerase Chain Reaction Porphyromonas gingivalis/classification/*genetics Sequence Analysis, DNA
Remarks: The ribosomal intergenic spacer regions (ISRs) of 19 laboratory strains and 30 clinical samples of Porphyromonas gingivalis were amplified by PCR and sequenced to provide a strain identifier. The ISR is a variable region of DNA located between the conserved 16S and 23S rRNA genes. This makes it an ideal locus for differentiation of strains within a species: primers specific for the conserved flanking genes were used to amplify the ISR, which was then sequenced to identify the strain. We have constructed a P. gingivalis ISR sequence database to facilitate strain identification. ISR sequence analysis provides a strain identifier that can be easily reproduced among laboratories and catalogued for unambiguous comparison.
URL: 10405432
Ref #: 69271
Author(s): Fournier,D.;Mouton,C.;Lapierre,P.;Kato,T.;Okuda,K.;Menard,C.
Journal: Int J Syst Evol Microbiol
Title: Porphyromonas gulae sp. nov., an anaerobic, gram-negative coccobacillus from the gingival sulcus of various animal hosts
Volume: 51
Page(s): 1179-89
Year: 2001
Keyword(s): GENBANK/AF208290 GENBANK/AF285871 GENBANK/AF285872 GENBANK/AF285873 GENBANK/AF285874 GENBANK/AF287986 Animals Base Sequence Carnivora Cats DNA, Bacterial/genetics Dogs Geography Gingiva/*microbiology Haplorhini Humans Molecular Sequence Data Nucleic Acid Hybridization *Phylogeny Porphyromonas/*classification/genetics/isolation & purification Porphyromonas gingivalis/classification/genetics Random Amplified Polymorphic DNA Technique Sequence Homology, Nucleic Acid Ursidae
Remarks: A new species, Porphyromonas gulae sp. nov., is proposed to include strains isolated from the gingival sulcus of various animal hosts which are distinct from related strains of Porphyromonas gingivalis of human origin. This bacterium exhibits the following characteristics: black-pigmented colonies; asaccharolytic, obligate anaerobic growth; and Gram-negative, non-motile and non-spore-forming, rod-shaped cells. Colonies do not fluoresce under UV light. Vitamin K1 and haemin are required for growth. Cells haemagglutinate sheep erythrocytes. Major fatty acid end products are butyric acid, isovaleric acid, succinic acid and phenylacetic acid. Strains are catalase-positive and indole is produced. Alkaline phosphatase, trypsin-like and N-acetyl-beta-glucosaminidase activities are strong. A beta-galactosidase and a glutamylglutamic acid arylamidase are also present. The G+C content of the chromosomal DNA is 51 mol%. DNA-DNA homology data and 16S rRNA gene sequence analysis provide strong evidence that strains from the animal biotype of P. gingivalis represent a Porphyromonas species that is distinct from P. gingivalis. The type strain of P. gulae is Loup 1T (= ATCC 51700T = NCTC 13180T).
URL: 11411686
Ref #: 25447
Author(s): Conrads,G.;Citron,D.M.;Tyrrell,K.L.;Horz,H.P.;Goldstein,E.J.
Journal: Int J Syst Evol Microbiol
Title: 16S-23S rRNA gene internal transcribed spacer sequences for analysis of the phylogenetic relationships among species of the genus Porphyromonas
Volume: 55
Page(s): 607-13
Year: 2005
Keyword(s): GENBANK/AB034799 GENBANK/AB035460 GENBANK/AF208290 GENBANK/AY546472 GENBANK/AY546473 GENBANK/AY546474 GENBANK/AY546475 GENBANK/AY546476 GENBANK/AY546477 GENBANK/AY546478 GENBANK/AY546479 GENBANK/AY546480 GENBANK/AY546481 GENBANK/AY546482 GENBANK/AY546483 GENBANK/AY546484 GENBANK/AY546485 GENBANK/AY546486 GENBANK/AY546487 GENBANK/AY546488 GENBANK/AY546489 GENBANK/AY546490 Bacterial Typing Techniques DNA, Ribosomal Spacer/*analysis Genes, rRNA Humans Molecular Sequence Data *Phylogeny Porphyromonas/*classification/genetics RNA, Ribosomal, 16S/*genetics RNA, Ribosomal, 23S/*genetics Sequence Analysis, DNA Species Specificity
Remarks: The 16S-23S rRNA gene internal transcribed spacer (ITS) regions of 11 reference strains of Porphyromonas species, together with Bacteroides distasonis and Tannerella forsythensis, were analysed to examine interspecies relationships. Compared with the phylogenetic tree generated using 16S rRNA gene sequences, the resolution of the ITS sequence-based tree was higher, but species positioning and clustering were similar with both approaches. The recent separation of Porphyromonas gulae and Porphyromonas gingivalis into distinct species was confirmed by the ITS data. In addition, analysis of the ITS sequences of 24 clinical isolates of Porphyromonas asaccharolytica plus the type strain ATCC 25260(T) divided the sequences into two clusters, of which one was alpha-fucosidase-positive (like the type strain) while the other was alpha-fucosidase-negative. The latter resembled the previously studied unusual extra-oral isolates of 'Porphyromonas endodontalis-like organisms' (PELOs) which could therefore be called 'Porphyromonas asaccharolytica-like organisms' (PALOs), based on the genetic identification. Moreover, the proposal of alpha-fucosidase-negative P. asaccharolytica strains as a new species should also be considered.
URL: 15774632
Ref #: 13708
Author(s): Rumpf,R.W.;Griffen,A.L.;Wen,B.G.;Leys,E.J.
Journal: J Clin Microbiol
Title: Sequencing of the ribosomal intergenic spacer region for strain identification of Porphyromonas gingivalis
Volume: 37
Page(s): 2723-5
Year: 1999
Keyword(s): GENBANK/AF118633 GENBANK/AF118634 GENBANK/AF118635 *Genes, Bacterial *Genes, rRNA Polymerase Chain Reaction Porphyromonas gingivalis/classification/*genetics Sequence Analysis, DNA Support, U.S. Gov't, P.H.S.
Remarks: The ribosomal intergenic spacer regions (ISRs) of 19 laboratory strains and 30 clinical samples of Porphyromonas gingivalis were amplified by PCR and sequenced to provide a strain identifier. The ISR is a variable region of DNA located between the conserved 16S and 23S rRNA genes. This makes it an ideal locus for differentiation of strains within a species: primers specific for the conserved flanking genes were used to amplify the ISR, which was then sequenced to identify the strain. We have constructed a P. gingivalis ISR sequence database to facilitate strain identification. ISR sequence analysis provides a strain identifier that can be easily reproduced among laboratories and catalogued for unambiguous comparison.
URL: 99335567
Ref #: 13701
Author(s): Fournier,D.;Mouton,C.;Lapierre,P.;Kato,T.;Okuda,K.;Menard,C.
Journal: Int J Syst Evol Microbiol
Title: Porphyromonas gulae sp. nov., an anaerobic, gram-negative coccobacillus from the gingival sulcus of various animal hosts
Volume: 51
Page(s): 1179-89
Year: 2001
Keyword(s): GENBANK/AF208290 GENBANK/AF285871 GENBANK/AF285872 GENBANK/AF285873 GENBANK/AF285874 GENBANK/AF287986 Animal Base Sequence Bears Carnivora Cats DNA, Bacterial/genetics Dogs Geography Gingiva/*microbiology Haplorhini Human Molecular Sequence Data Nucleic Acid Hybridization *Phylogeny Porphyromonas/*classification/genetics/isolation & purification Porphyromonas gingivalis/classification/genetics Random Amplified Polymorphic DNA Technique Sequence Homology, Nucleic Acid Support, Non-U.S. Gov't
Remarks: A new species, Porphyromonas gulae sp. nov., is proposed to include strains isolated from the gingival sulcus of various animal hosts which are distinct from related strains of Porphyromonas gingivalis of human origin. This bacterium exhibits the following characteristics: black-pigmented colonies; asaccharolytic, obligate anaerobic growth; and Gram-negative, non-motile and non-spore-forming, rod-shaped cells. Colonies do not fluoresce under UV light. Vitamin K1 and haemin are required for growth. Cells haemagglutinate sheep erythrocytes. Major fatty acid end products are butyric acid, isovaleric acid, succinic acid and phenylacetic acid. Strains are catalase-positive and indole is produced. Alkaline phosphatase, trypsin-like and N-acetyl-beta-glucosaminidase activities are strong. A beta-galactosidase and a glutamylglutamic acid arylamidase are also present. The G+C content of the chromosomal DNA is 51 mol%. DNA-DNA homology data and 16S rRNA gene sequence analysis provide strong evidence that strains from the animal biotype of P. gingivalis represent a Porphyromonas species that is distinct from P. gingivalis. The type strain of P. gulae is Loup 1T (= ATCC 51700T = NCTC 13180T).
URL: 21304468
Data: (ATCC 33277) / Type strain / A. L. Coykendall, University of Connecticut / ATCC in 1985 / J. Slots / Human gingival sulcus / Coykendall, A. L. et al. (1980) Int. J. syst. Bact. 30, 559
Accession Date: 01/01/0
History: SLOTS J - ATCC 1985 - COYKENDALL A L UNIV OF CONNECTICUT RESTR:WHO CLASS 2
Authority: (Coykendall et al. 1980) Shah and Collins 1988
Depositor: COYKENDALL A L
Taxonomy: TaxLink: S2344 (Bacteroides gingivalis) - Date of change: 5/02/2003
Biosafety Responsibility: It is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country

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