Culture Collections

Bacteria and Mycoplasmas detail

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Bacteria Collection: Veillonella dispar

NCTC Number: NCTC 11831
Current Name: Veillonella dispar
Original Strain Reference: ERN
Other Collection No: ATCC 17748
Previous Catalogue Name: Veillonella dispar
Type Strain: Yes
Family: Veillonellaceae
Hazard Group (ACDP): 2
Release Restrictions: Terms & Conditions of Supply of Microbial Pathogens: Safety
Conditions for growth on solid media: Fastidious anaerobe agar, 48 hours, 37°C, anaerobic
Isolated From: human, human mouth 1950
Whole Genome Sequence: http://www.ebi.ac.uk/ena/data/view/ERS1353913
16S rRNA Gene Sequence: >gb|AF439639|ATCC 17748|Veillonella dispar 16S ribosomal RNA gene, partial sequence.| ggaagcttgcttcta... >gb|AY995770|ATCC 17748|Veillonella dispar strain ATCC 17748 16S ribosomal RNA gene,partial sequence.| gctggcggcgtgctt...
Extended Bibliography: showhide Show bibliography
Ref #: 92484
Author(s): Marchandin,H.;Teyssier,C.;Simeon De Buochberg,M.;Jean-Pierre,H.;Carriere,C.;Jumas-Bilak,E.
Journal: Microbiology
Title: Intra-chromosomal heterogeneity between the four 16S rRNA gene copies in the genus Veillonella: implications for phylogeny and taxonomy
Volume: 149
Page(s): 1493-501
Year: 2003
Keyword(s): Base Sequence Chromosomes, Bacterial/genetics DNA, Bacterial/genetics DNA, Ribosomal/genetics Gene Dosage *Genes, Bacterial Humans Molecular Sequence Data Nucleic Acid Conformation Phylogeny Polymorphism, Restriction Fragment Length RNA, Bacterial/chemistry/*genetics RNA, Ribosomal, 16S/chemistry/*genetics Species Specificity Variation (Genetics) Veillonella/*classification/*genetics
Remarks: Among the seven species characterized within the genus Veillonella, three (Veillonella dispar, Veillonella parvula and Veillonella atypica) have so far been isolated from human flora and during infectious processes. Sequencing and analysis of 16S rDNA (rrs) has been described as the best method for identification of Veillonella strains at the species level since phenotypic characteristics are unable to differentiate between species. rrs sequencing for the three species isolated from humans showed more than 98 % identity between them. Four rrs copies were found in the reference strains and in all the clinical isolates studied. The sequences of each rrs were determined for the clinical strain ADV 360.1, and they showed a relatively high level of heterogeneity (1.43 %). In the majority of cases, polymorphic positions corresponded to nucleotides allowing differentiation between the three species isolated from humans. Moreover, variability observed between rrs copies was higher than that between 16S rDNA sequences of V. parvula and V. dispar. Phylogenetic analysis showed that polymorphism between rrs copies affected the position of strain ADV 360.1 in the tree. Variable positions occurred in stems and loops belonging to variable and hypervariable regions of the 16S rRNA secondary structure but did not change the overall structure of the 16S rRNA. PCR-RFLP experiments performed on 27 clinical isolates of Veillonella sp. suggested that inter-rrs heterogeneity occurs widely among the members of the genus VEILLONELLA: These results, together with the lack of phenotypic criteria for species differentiation, give preliminary arguments for unification of V. dispar and V. parvula.
URL: 12777489
Data: (ATCC 17748) Type strain / ATCC in 1985 / Human mouth in 1950
Accession Date: 01/01/1985
Authority: (Rogosa 1965) Mays et al. 1982 (ALI)
Depositor: ATCC
Taxonomy: TaxLink: S3526 (Veillonella dispar (Rogosa 1965) Mays et al. 1982) - Date of change: 5/02/2003
Biosafety Responsibility: It is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country

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