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Bacteria Collection: Bifidobacterium longum subsp. infantis

NCTC Number: NCTC 11817
Current Name: Bifidobacterium longum subsp. infantis
Original Strain Reference: S12
Other Collection No: ATCC 15697; DSM 20088; S12
Previous Catalogue Name: Bifidobacterium infantis
Type Strain: Yes
Family: Bifidobacteriaceae
Hazard Group (ACDP): 2
Release Restrictions: Terms & Conditions of Supply of Microbial Pathogens: Safety
Conditions for growth on solid media: Fastidious anaerobe agar, 48 hours, 37°C, anaerobic
Isolated From: human, intestine of infant
Whole Genome Sequence: http://www.ebi.ac.uk/ena/data/view/ERS1497488
16S rRNA Gene Sequence: >gb|U09792|ATCC 15697|Bifidobacterium infantis ATCC 15697 16S-23S intergenic spacer,partial sequence.| tcacctcctttctac... >gb|U09518|ATCC15698|Bifidobacterium breve ATCC15698 16S-23S intergenic spacer.| tcacctcctttctac... >gb|X70974|ATCC 15697|B.infantis (CIP 6469) DNA sequence for 16S rRNA.| tttgtggagggttcg... >gb|X89111|ATCC 15697|Bifidobacterium sp. 16S and 23S rRNA genes and ITS.| agggttcgattctgg... >gb|X70972|ATCC 15698|B.breve (ATCC 15698) DNA sequence for 16S rRNA.| tttgtggagggttcg...
23S rRNA Gene Sequence: >gb|U09792|ATCC 15697|Bifidobacterium infantis ATCC 15697 16S-23S intergenic spacer,partial sequence.| tcacctcctttctac... >gb|U09518|ATCC15698|Bifidobacterium breve ATCC15698 16S-23S intergenic spacer.| tcacctcctttctac... >gb|X89111|ATCC 15697|Bifidobacterium sp. 16S and 23S rRNA genes and ITS.| agggttcgattctgg...
Bibliography: REUTER G 1963 ZENTBL BAKT PARASIT ENKDE I ABT ORIG 486
Extended Bibliography: showhide Show bibliography
Ref #: 95531
Author(s): Kok,R.G.;de Waal,A.;Schut,F.;Welling,G.W.;Weenk,G.;Hellingwerf,K.J.
Journal: Appl Environ Microbiol
Title: Specific detection and analysis of a probiotic Bifidobacterium strain in infant feces
Volume: 62
Page(s): 3668-72
Year: 1996
Keyword(s): GENBANK/X89111 Animals Bifidobacterium/genetics/growth & development/*isolation & purification DNA Primers DNA, Ribosomal/genetics Double-Blind Method Feces/*microbiology Fermentation Humans Infant Food/*microbiology Infant, Newborn Milk Molecular Sequence Data Polymerase Chain Reaction/*methods RNA, Ribosomal, 16S/genetics Sensitivity and Specificity Sequence Analysis, DNA Sequence Homology, Nucleic Acid
Remarks: For specific detection of the probiotic Bifidobacterium sp. strain LW420 in infant feces and for rapid quality control of this strain in culture, three strain-specific 16S rRNA gene-targeted primers have been developed. These primers allow specific detection of the organism via PCR. Specificity of the primers was determined in DNA samples isolated from single-strain and mixed cultures of bifidobacteria and in heterogenous fecal samples. The feasibility of this method for use in specific detection of probiotic strains was investigated through addition of Bifidobacterium sp. strain LW420 to infant instant milk formula (IMF) and PCR analyses of bacterial DNA isolated from feces of 17 newborn IMF-fed infants. In feces of all nine babies that had been fed with the probiotic IMF, the strain-specific PCR signal could be detected. No signal was found in feces of the eight infants that had been fed with a nonprobiotic IMF, demonstrating the specificity of the PCR method. All 17 infants developed a major fecal Bifidobacterium population already after 3 days, as determined through genus-specific and strain-specific PCR. Phenotypical screening of Bifidobacterium sp. strain LW420 and analysis of homology of the 16S rRNA gene sequence of this strain with that of other bifidobacteria deposited in databases do not allow positive classification of LW420 among the currently known species of Bifidobacterium.
URL: 8837422
Ref #: 95526
Author(s): Leblond-Bourget,N.;Philippe,H.;Mangin,I.;Decaris,B.
Journal: Int J Syst Bacteriol
Title: 16S rRNA and 16S to 23S internal transcribed spacer sequence analyses reveal inter- and intraspecific Bifidobacterium phylogeny
Volume: 46
Page(s): 102-11
Year: 1996
Keyword(s): GENBANK/L36967 GENBANK/L36968 GENBANK/X70971 GENBANK/X70972 GENBANK/X70973 Base Sequence Bifidobacterium/*classification/genetics DNA, Bacterial DNA, Ribosomal/*genetics Molecular Sequence Data Phylogeny Polymerase Chain Reaction Polymorphism, Genetic RNA, Ribosomal, 16S/*genetics RNA, Ribosomal, 23S/*genetics
Remarks: In the last few years many attempts have been made to differentiate more than 20 Bifidobacterium species. It has been recognized that identification of bifidobacterial species is problematic because of phenetic and genetic heterogeneities. In order to contribute to our understanding of Bifidobacterium taxonomy, we studied Bifidobacterium phylogeny by performing both 16S rRNA and 16S to 23S (16S-23S) internally transcribed spacer (ITS) sequence analyses. In this study, we determined 16S rRNA sequences of five Bifidobacterium strains representing four species, and compared them with the sequences available in the GenBank database, and used them to construct a distance tree and for a bootstrap analysis. Moreover, we determined the ITS sequences of 29 bifidobacterial strains representing 18 species and compared these sequences with each other. We constructed a phylogenetic tree based on these sequence data and compared this tree with the tree based on 16S rRNA sequence data. We found that the two trees were similar topologically, suggesting that the two types of molecules provided the same kind of phylogenetic information. However, while 16S rRNA sequences are a good tool to infer interspecific links, the 16S-23S rDNA spacer data allowed us to determine intraspecific relationships. Each of the strains was characterized by its own ITS sequence; hence, 16S-23S rRNA sequences are a good tool for strain identification. Moreover, a comparison of the ITS sequences allowed us to estimate that the maximum level of ITS divergence between strains belonging to the same species was 13%. Our data allowed us to confirm the validity of most of the Bifidobacterium species which we studied and to identify some classification errors. Finally, our results showed that Bifidobacterium strains have no tRNA genes in the 16S-23S spacer region.
URL: 8573484
Ref #: 13723
Author(s): Kok,R.G.;de Waal,A.;Schut,F.;Welling,G.W.;Weenk,G.;Hellingwerf,K.J.
Journal: Appl Environ Microbiol
Title: Specific detection and analysis of a probiotic Bifidobacterium strain in infant feces
Volume: 62
Page(s): 3668-72
Year: 1996
Keyword(s): GENBANK/X89111 Animal Bifidobacterium/genetics/growth & development/*isolation & purification DNA Primers DNA, Ribosomal/genetics Double-Blind Method Feces/*microbiology Fermentation Human Infant Food/*microbiology Infant, Newborn Milk Molecular Sequence Data Polymerase Chain Reaction/*methods RNA, Ribosomal, 16S/genetics Sensitivity and Specificity Sequence Analysis, DNA Sequence Homology, Nucleic Acid
Remarks: For specific detection of the probiotic Bifidobacterium sp. strain LW420 in infant feces and for rapid quality control of this strain in culture, three strain-specific 16S rRNA gene-targeted primers have been developed. These primers allow specific detection of the organism via PCR. Specificity of the primers was determined in DNA samples isolated from single-strain and mixed cultures of bifidobacteria and in heterogenous fecal samples. The feasibility of this method for use in specific detection of probiotic strains was investigated through addition of Bifidobacterium sp. strain LW420 to infant instant milk formula (IMF) and PCR analyses of bacterial DNA isolated from feces of 17 newborn IMF-fed infants. In feces of all nine babies that had been fed with the probiotic IMF, the strain-specific PCR signal could be detected. No signal was found in feces of the eight infants that had been fed with a nonprobiotic IMF, demonstrating the specificity of the PCR method. All 17 infants developed a major fecal Bifidobacterium population already after 3 days, as determined through genus-specific and strain-specific PCR. Phenotypical screening of Bifidobacterium sp. strain LW420 and analysis of homology of the 16S rRNA gene sequence of this strain with that of other bifidobacteria deposited in databases do not allow positive classification of LW420 among the currently known species of Bifidobacterium.
URL: 96434455
Ref #: 12251
Author(s): Leblond-Bourget,N.;Philippe,H.;Mangin,I.;Decaris,B.
Journal: Int J Syst Bacteriol
Title: 16S rRNA and 16S to 23S internal transcribed spacer sequence analyses reveal inter- and intraspecific Bifidobacterium phylogeny
Volume: 46
Page(s): 102-11
Year: 1996
Keyword(s): GENBANK/L36967 GENBANK/L36968 GENBANK/X70971 GENBANK/X70972 GENBANK/X70973 Base Sequence Bifidobacterium/*classification/genetics DNA, Bacterial DNA, Ribosomal/*genetics Molecular Sequence Data Phylogeny Polymerase Chain Reaction Polymorphism (Genetics) RNA, Ribosomal, 16S/*genetics RNA, Ribosomal, 23S/*genetics Support, Non-U.S. Gov't
Remarks: In the last few years many attempts have been made to differentiate more than 20 Bifidobacterium species. It has been recognized that identification of bifidobacterial species is problematic because of phenetic and genetic heterogeneities. In order to contribute to our understanding of Bifidobacterium taxonomy, we studied Bifidobacterium phylogeny by performing both 16S rRNA and 16S to 23S (16S-23S) internally transcribed spacer (ITS) sequence analyses. In this study, we determined 16S rRNA sequences of five Bifidobacterium strains representing four species, and compared them with the sequences available in the GenBank database, and used them to construct a distance tree and for a bootstrap analysis. Moreover, we determined the ITS sequences of 29 bifidobacterial strains representing 18 species and compared these sequences with each other. We constructed a phylogenetic tree based on these sequence data and compared this tree with the tree based on 16S rRNA sequence data. We found that the two trees were similar topologically, suggesting that the two types of molecules provided the same kind of phylogenetic information. However, while 16S rRNA sequences are a good tool to infer interspecific links, the 16S-23S rDNA spacer data allowed us to determine intraspecific relationships. Each of the strains was characterized by its own ITS sequence; hence, 16S-23S rRNA sequences are a good tool for strain identification. Moreover, a comparison of the ITS sequences allowed us to estimate that the maximum level of ITS divergence between strains belonging to the same species was 13%. Our data allowed us to confirm the validity of most of the Bifidobacterium species which we studied and to identify some classification errors. Finally, our results showed that Bifidobacterium strains have no tRNA genes in the 16S-23S spacer region.
URL: 96138969
Ref #: 1300
Author(s): Skerman,V.B.D.;McGowan,V.;Sneath,P.H.A.(ed)
Journal: Int. J. Syst. Bacteriol.
Title: Approved Lists of Bacterial Names.
Volume: 30
Page(s): 225-420
Year: 1980
Ref #: 1379
Author(s): Lauer,E.;Kandler,O.
Journal: System. Appl. Microbiol.
Title: DNA-DNA homology, murein types and enzyme patterns in the type strains of the genus Bifidobacterium.
Volume: 4
Page(s): 42-64
Year: 1983
Ref #: 335
Author(s): Reuter,G.
Journal: Zentralbl. Bakteriol. Parasitenkd. Orig. Abt. I
Title: Vergleichende Untersuchungen über die Bifidus-Flora des Säuglings- und Erwachsenenstuhl.
Volume: 191
Page(s): 486-507
Year: 1963
Ref #: 418
Author(s): Werner,H.;Gasser,F.;Sebald,M.
Journal: Zentralbl. Bakteriol. Parasitenkd. Orig. Abt. I
Title: DNS-Basenbestimmungen an 28 Bifidus-Stämmen und an Stämmen morphologisch ähnlicher Gattungen.
Volume: 198
Page(s): 504-516
Year: 1965
Ref #: 490
Author(s): Scardovi,V.;Trovatelli,L.D.;Zani,G.;Crociani,F.;Matteuzzi,D.
Journal: Int. J. Syst. Bacteriol.
Title: Deoxyribonucleic acid homology relationships among species of the genus Bifidobacterium.
Volume: 21
Page(s): 276-294
Year: 1971
Data: (DSM 20088, ATCC 15698) Type strain / DSM in 1985 / G. Reuter / Intestine of infant / Reuter, G. (1963) Zentbl. Bakt. ParasitenKde. I. Abt. Orig. 191, 486
Accession Date: 01/01/1985
History: REUTER G - DSM 1985
Authority: Reuter 1963 (AL)
Depositor: DSM
Taxonomy: TaxLink: S558 (Bifidobacterium infantis) - Date of change: 5/02/2003
Biosafety Responsibility: It is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country

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