| Extended Bibliography: |
Show bibliography
| Ref #: |
95531 |
| Author(s): |
Kok,R.G.;de Waal,A.;Schut,F.;Welling,G.W.;Weenk,G.;Hellingwerf,K.J. |
| Journal: |
Appl Environ Microbiol |
| Title: |
Specific detection and analysis of a probiotic Bifidobacterium strain in infant feces |
| Volume: |
62 |
| Page(s): |
3668-72 |
| Year: |
1996 |
| Keyword(s): |
GENBANK/X89111
Animals
Bifidobacterium/genetics/growth & development/*isolation & purification
DNA Primers
DNA, Ribosomal/genetics
Double-Blind Method
Feces/*microbiology
Fermentation
Humans
Infant Food/*microbiology
Infant, Newborn
Milk
Molecular Sequence Data
Polymerase Chain Reaction/*methods
RNA, Ribosomal, 16S/genetics
Sensitivity and Specificity
Sequence Analysis, DNA
Sequence Homology, Nucleic Acid
|
| Remarks: |
For specific detection of the probiotic Bifidobacterium sp. strain LW420 in infant feces and for rapid quality control of this strain in culture, three strain-specific 16S rRNA gene-targeted primers have been developed. These primers allow specific detection of the organism via PCR. Specificity of the primers was determined in DNA samples isolated from single-strain and mixed cultures of bifidobacteria and in heterogenous fecal samples. The feasibility of this method for use in specific detection of probiotic strains was investigated through addition of Bifidobacterium sp. strain LW420 to infant instant milk formula (IMF) and PCR analyses of bacterial DNA isolated from feces of 17 newborn IMF-fed infants. In feces of all nine babies that had been fed with the probiotic IMF, the strain-specific PCR signal could be detected. No signal was found in feces of the eight infants that had been fed with a nonprobiotic IMF, demonstrating the specificity of the PCR method. All 17 infants developed a major fecal Bifidobacterium population already after 3 days, as determined through genus-specific and strain-specific PCR. Phenotypical screening of Bifidobacterium sp. strain LW420 and analysis of homology of the 16S rRNA gene sequence of this strain with that of other bifidobacteria deposited in databases do not allow positive classification of LW420 among the currently known species of Bifidobacterium. |
| URL: |
8837422 |
|
| Ref #: |
95526 |
| Author(s): |
Leblond-Bourget,N.;Philippe,H.;Mangin,I.;Decaris,B. |
| Journal: |
Int J Syst Bacteriol |
| Title: |
16S rRNA and 16S to 23S internal transcribed spacer sequence analyses reveal inter- and intraspecific Bifidobacterium phylogeny |
| Volume: |
46 |
| Page(s): |
102-11 |
| Year: |
1996 |
| Keyword(s): |
GENBANK/L36967
GENBANK/L36968
GENBANK/X70971
GENBANK/X70972
GENBANK/X70973
Base Sequence
Bifidobacterium/*classification/genetics
DNA, Bacterial
DNA, Ribosomal/*genetics
Molecular Sequence Data
Phylogeny
Polymerase Chain Reaction
Polymorphism, Genetic
RNA, Ribosomal, 16S/*genetics
RNA, Ribosomal, 23S/*genetics
|
| Remarks: |
In the last few years many attempts have been made to differentiate more than 20 Bifidobacterium species. It has been recognized that identification of bifidobacterial species is problematic because of phenetic and genetic heterogeneities. In order to contribute to our understanding of Bifidobacterium taxonomy, we studied Bifidobacterium phylogeny by performing both 16S rRNA and 16S to 23S (16S-23S) internally transcribed spacer (ITS) sequence analyses. In this study, we determined 16S rRNA sequences of five Bifidobacterium strains representing four species, and compared them with the sequences available in the GenBank database, and used them to construct a distance tree and for a bootstrap analysis. Moreover, we determined the ITS sequences of 29 bifidobacterial strains representing 18 species and compared these sequences with each other. We constructed a phylogenetic tree based on these sequence data and compared this tree with the tree based on 16S rRNA sequence data. We found that the two trees were similar topologically, suggesting that the two types of molecules provided the same kind of phylogenetic information. However, while 16S rRNA sequences are a good tool to infer interspecific links, the 16S-23S rDNA spacer data allowed us to determine intraspecific relationships. Each of the strains was characterized by its own ITS sequence; hence, 16S-23S rRNA sequences are a good tool for strain identification. Moreover, a comparison of the ITS sequences allowed us to estimate that the maximum level of ITS divergence between strains belonging to the same species was 13%. Our data allowed us to confirm the validity of most of the Bifidobacterium species which we studied and to identify some classification errors. Finally, our results showed that Bifidobacterium strains have no tRNA genes in the 16S-23S spacer region. |
| URL: |
8573484 |
|
| Ref #: |
13723 |
| Author(s): |
Kok,R.G.;de Waal,A.;Schut,F.;Welling,G.W.;Weenk,G.;Hellingwerf,K.J. |
| Journal: |
Appl Environ Microbiol |
| Title: |
Specific detection and analysis of a probiotic Bifidobacterium strain in infant feces |
| Volume: |
62 |
| Page(s): |
3668-72 |
| Year: |
1996 |
| Keyword(s): |
GENBANK/X89111
Animal
Bifidobacterium/genetics/growth & development/*isolation & purification
DNA Primers
DNA, Ribosomal/genetics
Double-Blind Method
Feces/*microbiology
Fermentation
Human
Infant Food/*microbiology
Infant, Newborn
Milk
Molecular Sequence Data
Polymerase Chain Reaction/*methods
RNA, Ribosomal, 16S/genetics
Sensitivity and Specificity
Sequence Analysis, DNA
Sequence Homology, Nucleic Acid
|
| Remarks: |
For specific detection of the probiotic Bifidobacterium sp. strain LW420 in infant feces and for rapid quality control of this strain in culture, three strain-specific 16S rRNA gene-targeted primers have been developed. These primers allow specific detection of the organism via PCR. Specificity of the primers was determined in DNA samples isolated from single-strain and mixed cultures of bifidobacteria and in heterogenous fecal samples. The feasibility of this method for use in specific detection of probiotic strains was investigated through addition of Bifidobacterium sp. strain LW420 to infant instant milk formula (IMF) and PCR analyses of bacterial DNA isolated from feces of 17 newborn IMF-fed infants. In feces of all nine babies that had been fed with the probiotic IMF, the strain-specific PCR signal could be detected. No signal was found in feces of the eight infants that had been fed with a nonprobiotic IMF, demonstrating the specificity of the PCR method. All 17 infants developed a major fecal Bifidobacterium population already after 3 days, as determined through genus-specific and strain-specific PCR. Phenotypical screening of Bifidobacterium sp. strain LW420 and analysis of homology of the 16S rRNA gene sequence of this strain with that of other bifidobacteria deposited in databases do not allow positive classification of LW420 among the currently known species of Bifidobacterium. |
| URL: |
96434455 |
|
| Ref #: |
12251 |
| Author(s): |
Leblond-Bourget,N.;Philippe,H.;Mangin,I.;Decaris,B. |
| Journal: |
Int J Syst Bacteriol |
| Title: |
16S rRNA and 16S to 23S internal transcribed spacer sequence analyses reveal inter- and intraspecific Bifidobacterium phylogeny |
| Volume: |
46 |
| Page(s): |
102-11 |
| Year: |
1996 |
| Keyword(s): |
GENBANK/L36967
GENBANK/L36968
GENBANK/X70971
GENBANK/X70972
GENBANK/X70973
Base Sequence
Bifidobacterium/*classification/genetics
DNA, Bacterial
DNA, Ribosomal/*genetics
Molecular Sequence Data
Phylogeny
Polymerase Chain Reaction
Polymorphism (Genetics)
RNA, Ribosomal, 16S/*genetics
RNA, Ribosomal, 23S/*genetics
Support, Non-U.S. Gov't
|
| Remarks: |
In the last few years many attempts have been made to differentiate more than 20 Bifidobacterium species. It has been recognized that identification of bifidobacterial species is problematic because of phenetic and genetic heterogeneities. In order to contribute to our understanding of Bifidobacterium taxonomy, we studied Bifidobacterium phylogeny by performing both 16S rRNA and 16S to 23S (16S-23S) internally transcribed spacer (ITS) sequence analyses. In this study, we determined 16S rRNA sequences of five Bifidobacterium strains representing four species, and compared them with the sequences available in the GenBank database, and used them to construct a distance tree and for a bootstrap analysis. Moreover, we determined the ITS sequences of 29 bifidobacterial strains representing 18 species and compared these sequences with each other. We constructed a phylogenetic tree based on these sequence data and compared this tree with the tree based on 16S rRNA sequence data. We found that the two trees were similar topologically, suggesting that the two types of molecules provided the same kind of phylogenetic information. However, while 16S rRNA sequences are a good tool to infer interspecific links, the 16S-23S rDNA spacer data allowed us to determine intraspecific relationships. Each of the strains was characterized by its own ITS sequence; hence, 16S-23S rRNA sequences are a good tool for strain identification. Moreover, a comparison of the ITS sequences allowed us to estimate that the maximum level of ITS divergence between strains belonging to the same species was 13%. Our data allowed us to confirm the validity of most of the Bifidobacterium species which we studied and to identify some classification errors. Finally, our results showed that Bifidobacterium strains have no tRNA genes in the 16S-23S spacer region. |
| URL: |
96138969 |
|
| Ref #: |
1300 |
| Author(s): |
Skerman,V.B.D.;McGowan,V.;Sneath,P.H.A.(ed) |
| Journal: |
Int. J. Syst. Bacteriol. |
| Title: |
Approved Lists of Bacterial Names. |
| Volume: |
30 |
| Page(s): |
225-420 |
| Year: |
1980 |
|
| Ref #: |
1379 |
| Author(s): |
Lauer,E.;Kandler,O. |
| Journal: |
System. Appl. Microbiol. |
| Title: |
DNA-DNA homology, murein types and enzyme patterns in the type strains of the genus Bifidobacterium. |
| Volume: |
4 |
| Page(s): |
42-64 |
| Year: |
1983 |
|
| Ref #: |
335 |
| Author(s): |
Reuter,G. |
| Journal: |
Zentralbl. Bakteriol. Parasitenkd. Orig. Abt. I |
| Title: |
Vergleichende Untersuchungen über die Bifidus-Flora des Säuglings- und Erwachsenenstuhl. |
| Volume: |
191 |
| Page(s): |
486-507 |
| Year: |
1963 |
|
| Ref #: |
418 |
| Author(s): |
Werner,H.;Gasser,F.;Sebald,M. |
| Journal: |
Zentralbl. Bakteriol. Parasitenkd. Orig. Abt. I |
| Title: |
DNS-Basenbestimmungen an 28 Bifidus-Stämmen und an Stämmen morphologisch ähnlicher Gattungen. |
| Volume: |
198 |
| Page(s): |
504-516 |
| Year: |
1965 |
|
| Ref #: |
490 |
| Author(s): |
Scardovi,V.;Trovatelli,L.D.;Zani,G.;Crociani,F.;Matteuzzi,D. |
| Journal: |
Int. J. Syst. Bacteriol. |
| Title: |
Deoxyribonucleic acid homology relationships among species of the genus Bifidobacterium. |
| Volume: |
21 |
| Page(s): |
276-294 |
| Year: |
1971 |
|
|
Product Documents
Print datasheet