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Bacteria and Mycoplasmas detail

Conditions of Supply of Microbial Pathogens: Safety





Bacteria Collection: Bifidobacterium dentium

NCTC Number: NCTC 11816
Current Name: Bifidobacterium dentium
Original Strain Reference: B764
Other Collection No: ATCC 27534; B764; DSM 20436
Previous Catalogue Name: Bifidobacterium dentium
Type Strain: Yes
Family: Bifidobacteriaceae
Hazard Group (ACDP): 2
Release Restrictions: Terms & Conditions of Supply of Microbial Pathogens: Safety
Conditions for growth on solid media: Columbia blood agar, 48 hours, 37°C, anaerobic
Isolated From: human, dental caries
Whole Genome Sequence: http://www.ebi.ac.uk/ena/data/view/ERS1247832
16S rRNA Gene Sequence: >gb|U10434|ATCC 27534|Bifidobacterium dentium ATCC 27534 16S-23S rRNA intergenic spacer.| tcacctcctttctac...
23S rRNA Gene Sequence: >gb|U10434|ATCC 27534|Bifidobacterium dentium ATCC 27534 16S-23S rRNA intergenic spacer.| tcacctcctttctac...
Bibliography: SCARDOVI V & CROCIANI F 1974 INT J SYST BACT 24 6
Extended Bibliography: showhide Show bibliography
Ref #: 95526
Author(s): Leblond-Bourget,N.;Philippe,H.;Mangin,I.;Decaris,B.
Journal: Int J Syst Bacteriol
Title: 16S rRNA and 16S to 23S internal transcribed spacer sequence analyses reveal inter- and intraspecific Bifidobacterium phylogeny
Volume: 46
Page(s): 102-11
Year: 1996
Keyword(s): GENBANK/L36967 GENBANK/L36968 GENBANK/X70971 GENBANK/X70972 GENBANK/X70973 Base Sequence Bifidobacterium/*classification/genetics DNA, Bacterial DNA, Ribosomal/*genetics Molecular Sequence Data Phylogeny Polymerase Chain Reaction Polymorphism, Genetic RNA, Ribosomal, 16S/*genetics RNA, Ribosomal, 23S/*genetics
Remarks: In the last few years many attempts have been made to differentiate more than 20 Bifidobacterium species. It has been recognized that identification of bifidobacterial species is problematic because of phenetic and genetic heterogeneities. In order to contribute to our understanding of Bifidobacterium taxonomy, we studied Bifidobacterium phylogeny by performing both 16S rRNA and 16S to 23S (16S-23S) internally transcribed spacer (ITS) sequence analyses. In this study, we determined 16S rRNA sequences of five Bifidobacterium strains representing four species, and compared them with the sequences available in the GenBank database, and used them to construct a distance tree and for a bootstrap analysis. Moreover, we determined the ITS sequences of 29 bifidobacterial strains representing 18 species and compared these sequences with each other. We constructed a phylogenetic tree based on these sequence data and compared this tree with the tree based on 16S rRNA sequence data. We found that the two trees were similar topologically, suggesting that the two types of molecules provided the same kind of phylogenetic information. However, while 16S rRNA sequences are a good tool to infer interspecific links, the 16S-23S rDNA spacer data allowed us to determine intraspecific relationships. Each of the strains was characterized by its own ITS sequence; hence, 16S-23S rRNA sequences are a good tool for strain identification. Moreover, a comparison of the ITS sequences allowed us to estimate that the maximum level of ITS divergence between strains belonging to the same species was 13%. Our data allowed us to confirm the validity of most of the Bifidobacterium species which we studied and to identify some classification errors. Finally, our results showed that Bifidobacterium strains have no tRNA genes in the 16S-23S spacer region.
URL: 8573484
Ref #: 12251
Author(s): Leblond-Bourget,N.;Philippe,H.;Mangin,I.;Decaris,B.
Journal: Int J Syst Bacteriol
Title: 16S rRNA and 16S to 23S internal transcribed spacer sequence analyses reveal inter- and intraspecific Bifidobacterium phylogeny
Volume: 46
Page(s): 102-11
Year: 1996
Keyword(s): GENBANK/L36967 GENBANK/L36968 GENBANK/X70971 GENBANK/X70972 GENBANK/X70973 Base Sequence Bifidobacterium/*classification/genetics DNA, Bacterial DNA, Ribosomal/*genetics Molecular Sequence Data Phylogeny Polymerase Chain Reaction Polymorphism (Genetics) RNA, Ribosomal, 16S/*genetics RNA, Ribosomal, 23S/*genetics Support, Non-U.S. Gov't
Remarks: In the last few years many attempts have been made to differentiate more than 20 Bifidobacterium species. It has been recognized that identification of bifidobacterial species is problematic because of phenetic and genetic heterogeneities. In order to contribute to our understanding of Bifidobacterium taxonomy, we studied Bifidobacterium phylogeny by performing both 16S rRNA and 16S to 23S (16S-23S) internally transcribed spacer (ITS) sequence analyses. In this study, we determined 16S rRNA sequences of five Bifidobacterium strains representing four species, and compared them with the sequences available in the GenBank database, and used them to construct a distance tree and for a bootstrap analysis. Moreover, we determined the ITS sequences of 29 bifidobacterial strains representing 18 species and compared these sequences with each other. We constructed a phylogenetic tree based on these sequence data and compared this tree with the tree based on 16S rRNA sequence data. We found that the two trees were similar topologically, suggesting that the two types of molecules provided the same kind of phylogenetic information. However, while 16S rRNA sequences are a good tool to infer interspecific links, the 16S-23S rDNA spacer data allowed us to determine intraspecific relationships. Each of the strains was characterized by its own ITS sequence; hence, 16S-23S rRNA sequences are a good tool for strain identification. Moreover, a comparison of the ITS sequences allowed us to estimate that the maximum level of ITS divergence between strains belonging to the same species was 13%. Our data allowed us to confirm the validity of most of the Bifidobacterium species which we studied and to identify some classification errors. Finally, our results showed that Bifidobacterium strains have no tRNA genes in the 16S-23S spacer region.
URL: 96138969
Data: (DSM 20436, ATCC 27534) Type strain / DSM in 1985 / ATCC / V. Scardovi / Dental caries / Scardovi, V. & Crociani, F. (1974) Int. J. syst. Bact. 24, 6
Accession Date: 01/01/1985
History: SCARDOVI V - ATCC - DSM
Authority: Scardovi and Crociani 1974 (AL)
Depositor: DSM
Taxonomy: TaxLink: S553 (Bifidobacterium dentium Scardovi and Crociani 1974) - Date of change: 5/02/2003
Biosafety Responsibility: It is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country

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