| NCTC Number: |
NCTC 11800
|
| Current Name: |
Providencia stuartii
|
| Original Strain Reference: |
CDC 2896-68
|
| Other Collection No: |
ATCC 29914; CDC 2896-68; DSM 4539
|
| Previous Catalogue Name: |
Providencia stuartii
|
| Type Strain: |
Yes
|
| Family: |
Enterobacteriaceae
|
| Hazard Group (ACDP): |
2
|
| Release Restrictions: |
Terms & Conditions of Supply of Microbial Pathogens: Safety
|
| Conditions for growth on solid media: |
Nutrient agar, 24 hours, 37°C, aerobic
|
| Conditions for growth on liquid media: |
nutrient broth,37, facultative anaerobe
|
| Isolated From: |
human
|
| Whole Genome Sequence: |
http://www.ebi.ac.uk/ena/data/view/ERS513146
|
| Annotated Genome: |
ftp://ftp.sanger.ac.uk/pub/project/pathogens/NCTC3000/d...
|
| 16S rRNA Gene Sequence: |
>gb|AF008581|ATCC 29914T|Providencia stuartii 16S ribosomal RNA gene, partial sequence.| tgatcctggctcaga...
|
| Miscellaneous Sequence Data: |
>gb|AY370859|ATCC 29914|Providencia stuartii strain ATCC 29914 gyrase B (gyrB) gene,partial cds.| aaagtctccggcggt...
|
| Extended Bibliography: |
Show bibliography
| Ref #: |
95511 |
| Author(s): |
Mollet,C.;Drancourt,M.;Raoult,D. |
| Journal: |
Mol Microbiol |
| Title: |
rpoB sequence analysis as a novel basis for bacterial identification |
| Volume: |
26 |
| Page(s): |
1005-11 |
| Year: |
1998 |
| Keyword(s): |
GENBANK/AF008577
GENBANK/AF008579
GENBANK/AF008581
GENBANK/AF008582
GENBANK/U77434
GENBANK/U77435
GENBANK/U77436
GENBANK/U77437
GENBANK/U77438
GENBANK/U77439
GENBANK/U77440
GENBANK/U77441
GENBANK/U77443
GENBANK/U77445
GENBANK/U77446
GENBANK/U77447
GENBANK/U77448
GENBANK/U77449
GENBANK/U77450
GENBANK/U77451
GENBANK/U77452
GENBANK/U77453
GENBANK/U78182
GENBANK/U78183
GENBANK/X13854
DNA-Directed RNA Polymerases/*genetics
Databases, Factual
Enterobacteriaceae/genetics
Evolution, Molecular
RNA, Ribosomal, 16S
*Sequence Analysis, DNA
|
| Remarks: |
Comparison of the sequences of conserved genes, most commonly those encoding 16S rRNA, is used for bacterial genotypic identification. Among some taxa, such as the Enterobacteriaceae, variation within this gene does not allow confident species identification. We investigated the usefulness of RNA polymerase beta-subunit encoding gene (rpoB) sequences as an alternative tool for universal bacterial genotypic identification. We generated a database of partial rpoB for 14 Enterobacteriaceae species and then assessed the intra- and interspecies divergence between the rpoB and the 16S rRNA genes by pairwise comparisons. We found that levels of divergence between the rpoB sequences of different strains were markedly higher than those between their 16S rRNA genes. This higher discriminatory power was further confirmed by assigning 20 blindly selected clinical isolates to the correct enteric species on the basis of rpoB sequence comparison. Comparison of rpoB sequences from Enterobacteriaceae was also used as the basis for their phylogenetic analysis and demonstrated the genus Klebsiella to be polyphyletic. The trees obtained with rpoB were more compatible with the currently accepted classification of Enterobacteriaceae than those obtained with 16S rRNA. These data indicate that rpoB is a powerful identification tool, which may be useful for universal bacterial identification. |
| URL: |
9426137 |
|
| Ref #: |
43181 |
| Author(s): |
Delmas,J.;Breysse,F.;Devulder,G.;Flandrois,J.P.;Chomarat,M. |
| Journal: |
Diagn Microbiol Infect Dis |
| Title: |
Rapid identification of Enterobacteriaceae by sequencing DNA gyrase subunit B encoding gene |
| Volume: |
55 |
| Page(s): |
263-8 |
| Year: |
2006 |
| Keyword(s): |
Bacterial Typing Techniques/*methods
DNA Gyrase/*genetics
Enterobacteriaceae/enzymology/genetics/*isolation & purification
Genotype
Humans
Sequence Analysis, DNA/methods
|
| Remarks: |
Real-time polymerase chain reaction and sequencing were used to characterize a 506-bp-long DNA fragment internal to the gyrB gene (gyrBint). The sequences obtained from 32 Enterobacteriaceae-type strains and those available in the Genbank nucleotide sequence database (n = 24) were used as a database to identify 240 clinical enterobacteria isolates. Sequence analysis of the gyrBint fragment of 240 strains showed that gyrBint constitutes a discriminative target sequence to differentiate between Enterobacteriaceae species. Comparison of these identifications with those obtained by phenotypic methods (Vitek 1 system and/or Rapid ID 32E; bioMerieux, Marcy l'Etoile, France) revealed discrepancies essentially with genera Citrobacter and Enterobacter. Most of the strains identified as Enterobacter cloacae by phenotypic methods were identified as Enterobacter hormaechei strains by gyrBint sequencing. The direct sequencing of gyrBint would be useful as a complementary tool in the identification of clinical Enterobacteriaceae isolates. |
| URL: |
16626902 |
|
| Ref #: |
12921 |
| Author(s): |
Mollet,C.;Drancourt,M.;Raoult,D. |
| Journal: |
Mol Microbiol |
| Title: |
rpoB sequence analysis as a novel basis for bacterial identification |
| Volume: |
26 |
| Page(s): |
1005-11 |
| Year: |
1998 |
| Keyword(s): |
GENBANK/AF008577
GENBANK/AF008579
GENBANK/AF008581
GENBANK/AF008582
GENBANK/U77434
GENBANK/U77435
GENBANK/U77436
GENBANK/U77437
GENBANK/U77438
GENBANK/U77439
GENBANK/U77440
GENBANK/U77441
GENBANK/U77443
GENBANK/U77445
GENBANK/U77446
GENBANK/U77447
GENBANK/U77448
GENBANK/U77449
GENBANK/U77450
GENBANK/U77451
GENBANK/U77452
GENBANK/U77453
GENBANK/U78182
GENBANK/U78183
GENBANK/X13854
DNA-Directed RNA Polymerases/*genetics
Databases, Factual
Enterobacteriaceae/genetics
Evolution, Molecular
RNA, Ribosomal, 16S
*Sequence Analysis, DNA
Support, Non-U.S. Gov't
|
| Remarks: |
Comparison of the sequences of conserved genes, most commonly those encoding 16S rRNA, is used for bacterial genotypic identification. Among some taxa, such as the Enterobacteriaceae, variation within this gene does not allow confident species identification. We investigated the usefulness of RNA polymerase beta-subunit encoding gene (rpoB) sequences as an alternative tool for universal bacterial genotypic identification. We generated a database of partial rpoB for 14 Enterobacteriaceae species and then assessed the intra- and interspecies divergence between the rpoB and the 16S rRNA genes by pairwise comparisons. We found that levels of divergence between the rpoB sequences of different strains were markedly higher than those between their 16S rRNA genes. This higher discriminatory power was further confirmed by assigning 20 blindly selected clinical isolates to the correct enteric species on the basis of rpoB sequence comparison. Comparison of rpoB sequences from Enterobacteriaceae was also used as the basis for their phylogenetic analysis and demonstrated the genus Klebsiella to be polyphyletic. The trees obtained with rpoB were more compatible with the currently accepted classification of Enterobacteriaceae than those obtained with 16S rRNA. These data indicate that rpoB is a powerful identification tool, which may be useful for universal bacterial identification. |
| URL: |
98086106 |
|
|
| Data: |
(ATCC 29914) Type strain / ATCC in 1984 / CDC Atlanta / Colorado State Health Department / Human, 1968 Biogroup 5
|
| Accession Date: |
01/01/1984
|
| History: |
COLORADO STATE HEALTH DEPT - CDC - ATCC
|
| Authority: |
(Buttiaux et al. 1954) Ewing 1962 (AL)
|
| Depositor: |
ATCC
|
| Taxonomy: |
TaxLink: S2395 (Providencia stuartii (Buttiaux et al. 1954) Ewing 1962) - Date of change: 5/02/2003
|
| Biosafety Responsibility: |
It is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country
|
The Culture Collections hold cell cultures, bacteria, fungi and virus strains from worldwide sources. Our scientists ensure that the identification of the cultures is correct and they remain unchanged from when they are first deposited with the Collection. Nevertheless, some of the data we provide about the cultures is supplied by the person depositing the strains and, although we have multiple checking procedures in place, we cannot always verify all their data. Please note that the Culture Collections cannot be held responsible for any inaccuracies in the data provided by the depositors.
Cultures supplied by Culture Collections are to be used as controls for microbiology testing and for research purposes only. Please view the Terms & Conditions of Supply for more information.
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