Extended Bibliography: |
Show bibliography
Ref #: |
58580 |
Author(s): |
Tiveljung,A.;Backstrom,J.;Forsum,U.;Monstein,H.J. |
Journal: |
APMIS |
Title: |
Broad-range PCR amplification and DNA sequence analysis reveals variable motifs in 16S rRNA genes of Mobiluncus species |
Volume: |
103 |
Page(s): |
755-63 |
Year: |
1996 |
Keyword(s): |
GENBANK/X82602
GENBANK/X82603
GENBANK/X82604
GENBANK/X82605
GENBANK/X82606
GENBANK/X82607
GENBANK/X82608
GENBANK/X82609
GENBANK/X82610
GENBANK/X82611
GENBANK/X86004
GENBANK/X86005
GENBANK/X86006
Base Sequence
DNA, Ribosomal/*genetics
Mobiluncus/*genetics
Molecular Sequence Data
RNA, Ribosomal, 16S/*genetics
Sequence Alignment
Sequence Analysis
|
Remarks: |
Using DNA primers based on highly conserved regions of bacterial 16S ribosomal RNA genes, a technique was established for detection of Mobiluncus species by polymerase chain reaction (PCR) and hybridization analysis. Part of the 16S rRNA genes of Mobiluncus mulieris, Mobiluncus curtisii and uncharacterized Mobiluncus strains were analyzed by broad-range PCR amplification and direct DNA sequencing analysis. Sequence comparison of the partial 16S rRNA genes of Mobiluncus curtisii, Mobiluncus mulieris and atypical Mobiluncus strains studied indicated genus and species-specific motifs within the variable regions V3, V4 and V9 of 16S ribosomal DNAs. A Mobiluncus curtisii-specific primer, located within the variable region V3 of the 16S rRNA gene, was designed for Southern blot hybridization analysis of broad-range PCR products. Broad-range amplification combined with a M. curtisii-specific hybridization probe, Mob V3, distinguished between Mobiluncus curtisii, Mobiluncus mulieris, and atypical Mobiluncus strains. |
URL: |
8534436 |
|
Ref #: |
58579 |
Author(s): |
Tiveljung,A.;Forsum,U.;Monstein,H.J. |
Journal: |
Int J Syst Bacteriol |
Title: |
Classification of the genus Mobiluncus based on comparative partial 16S rRNA gene analysis |
Volume: |
46 |
Page(s): |
332-6 |
Year: |
1996 |
Keyword(s): |
GENBANK/X82602
GENBANK/X82603
GENBANK/X82604
GENBANK/X82605
GENBANK/X82606
GENBANK/X82607
GENBANK/X82608
GENBANK/X86004
GENBANK/X86005
GENBANK/X86006
Base Sequence
Blotting, Southern
DNA, Bacterial/genetics
Genes, Bacterial
Mobiluncus/*classification/genetics
Molecular Sequence Data
RNA, Bacterial/genetics
RNA, Ribosomal, 16S/*genetics
Sequence Homology, Nucleic Acid
|
Remarks: |
On the basis of partial 16S rRNA gene sequences and the results of Southern blot analyses, we confirmed the division of the genus Mobiluncus into the species Mobiluncus curtisii and Mobiluncus mulieris. Division of M. curtisii into M. curtisii subsp. curtisii and M. curtisii subsp. holmesii was not supported by our data. |
URL: |
8573515 |
|
Ref #: |
13726 |
Author(s): |
Tiveljung,A.;Backstrom,J.;Forsum,U.;Monstein,H.J. |
Journal: |
APMIS |
Title: |
Broad-range PCR amplification and DNA sequence analysis reveals variable motifs in 16S rRNA genes of Mobiluncus species |
Volume: |
103 |
Page(s): |
755-63 |
Year: |
1996 |
Keyword(s): |
GENBANK/X82602
GENBANK/X82603
GENBANK/X82604
GENBANK/X82605
GENBANK/X82606
GENBANK/X82607
GENBANK/X82608
GENBANK/X82609
GENBANK/X82610
GENBANK/X82611
GENBANK/X86004
GENBANK/X86005
GENBANK/X86006
Base Sequence
DNA, Ribosomal/*genetics
Mobiluncus/*genetics
Molecular Sequence Data
RNA, Ribosomal, 16S/*genetics
Sequence Alignment
Sequence Analysis
Support, Non-U.S. Gov't
|
Remarks: |
Using DNA primers based on highly conserved regions of bacterial 16S ribosomal RNA genes, a technique was established for detection of Mobiluncus species by polymerase chain reaction (PCR) and hybridization analysis. Part of the 16S rRNA genes of Mobiluncus mulieris, Mobiluncus curtisii and uncharacterized Mobiluncus strains were analyzed by broad-range PCR amplification and direct DNA sequencing analysis. Sequence comparison of the partial 16S rRNA genes of Mobiluncus curtisii, Mobiluncus mulieris and atypical Mobiluncus strains studied indicated genus and species-specific motifs within the variable regions V3, V4 and V9 of 16S ribosomal DNAs. A Mobiluncus curtisii-specific primer, located within the variable region V3 of the 16S rRNA gene, was designed for Southern blot hybridization analysis of broad-range PCR products. Broad-range amplification combined with a M. curtisii-specific hybridization probe, Mob V3, distinguished between Mobiluncus curtisii, Mobiluncus mulieris, and atypical Mobiluncus strains. |
URL: |
96125706 |
|
Ref #: |
13725 |
Author(s): |
Tiveljung,A.;Forsum,U.;Monstein,H.J. |
Journal: |
Int J Syst Bacteriol |
Title: |
Classification of the genus Mobiluncus based on comparative partial 16S rRNA gene analysis |
Volume: |
46 |
Page(s): |
332-6 |
Year: |
1996 |
Keyword(s): |
GENBANK/X82602
GENBANK/X82603
GENBANK/X82604
GENBANK/X82605
GENBANK/X82606
GENBANK/X82607
GENBANK/X82608
GENBANK/X86004
GENBANK/X86005
GENBANK/X86006
Base Sequence
Blotting, Southern
Comparative Study
DNA, Bacterial/genetics
Genes, Bacterial
Mobiluncus/*classification/genetics
Molecular Sequence Data
RNA, Bacterial/genetics
RNA, Ribosomal, 16S/*genetics
Sequence Homology, Nucleic Acid
Support, Non-U.S. Gov't
|
Remarks: |
On the basis of partial 16S rRNA gene sequences and the results of Southern blot analyses, we confirmed the division of the genus Mobiluncus into the species Mobiluncus curtisii and Mobiluncus mulieris. Division of M. curtisii into M. curtisii subsp. curtisii and M. curtisii subsp. holmesii was not supported by our data. |
URL: |
96138996 |
|
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