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Bacteria Collection: Grimontia hollisae

NCTC Number: NCTC 11640
Current Name: Grimontia hollisae
Original Strain Reference: 0075-80
Other Collection No: ATCC 33564; CDC 0075-80; DSM 15132; IMET 12291
Previous Catalogue Name: Vibrio hollisae
Type Strain: Yes
Family: Vibrionaceae
Hazard Group (ACDP): 2
Release Restrictions: Terms & Conditions of Supply of Microbial Pathogens: Safety
Conditions for growth on solid media: Columbia blood agar, 24-48 hours, 37°C, aerobic
Conditions for growth on liquid media: nutrient broth,37, facultative anaerobe
Isolated From: human faeces
Whole Genome Sequence: http://www.ebi.ac.uk/ena/data/view/ERS1295688
16S rRNA Gene Sequence: >gb|X56583 S83393 S83427|ATCC 33564|V.hollisae 16S ribosomal RNA.| naaattgaagagttt... >gb|X74707|ATCC 33564T|V.hollisae(ATCC 33564T) gene for 16S ribosomal RNA.| gagtttgatcatggc... >gb|DQ068939|ATCC 33564|Grimontia hollisae 16S ribosomal RNA gene, partial sequence.| gtcgagcggcagcga... >gb|X56583 AH000276 S83393 S83427 S83429|ATCC 33564|V.hollisae 16S ribosomal RNA.| naaattgaagagttt...
Bibliography: HICKMAN F W ET AL 1982 J CLIN MICRO 15 395; VALIDATION LIST NO 9 1982 INT
Extended Bibliography: showhide Show bibliography
Ref #: 93196
Author(s): Ruimy,R.;Breittmayer,V.;Elbaze,P.;Lafay,B.;Boussemart,O.;Gauthier,M.;Christen,R.
Journal: Int J Syst Bacteriol
Title: Phylogenetic analysis and assessment of the genera Vibrio, Photobacterium, Aeromonas, and Plesiomonas deduced from small-subunit rRNA sequences
Volume: 44
Page(s): 416-26
Year: 1994
Keyword(s): GENBANK/X74674 GENBANK/X74675 GENBANK/X74676 GENBANK/X74677 GENBANK/X74678 GENBANK/X74679 GENBANK/X74680 GENBANK/X74681 GENBANK/X74682 GENBANK/X74683 GENBANK/X74684 GENBANK/X74685 GENBANK/X74686 GENBANK/X74687 GENBANK/X74688 GENBANK/X74689 GENBANK/X74690 GENBANK/X74691 GENBANK/X74692 GENBANK/X74693 GENBANK/X74694 GENBANK/X74695 GENBANK/X74696 GENBANK/X74697 GENBANK/X74698 GENBANK/X74699 GENBANK/X74700 GENBANK/X74701 GENBANK/X74702 GENBANK/X74703 Aeromonas/classification/genetics Photobacterium/classification/genetics *Phylogeny Plesiomonas/classification/genetics RNA, Bacterial/*genetics RNA, Ribosomal/*genetics Sequence Homology, Nucleic Acid Species Specificity Vibrio/classification/genetics Vibrionaceae/*classification/*genetics
Remarks: We sequenced nearly complete small-subunit rRNAs of 54 reference strains belonging to the genera Vibrio, Photobacterium, Aeromonas, and Plesiomonas. We then performed a phylogenetic analysis by comparing the sequences which we obtained with all other known sequences for bacteria belonging to the gamma subgroup of the Proteobacteria (thus providing a data base consisting of 70 sequences for the genera investigated), using methods such as neighbor joining, maximum likelihood, and maximum parsimony, as well as bootstrap, to assess the robustness of each topology. Our results confirmed that the family Vibrionaceae should include only Photobacterium and Vibrio species (but not Vibrio marinus); that Aeromonas species deserve family rank; and that Plesiomonas shigelloides is linked to the family Enterobacteriaceae. The genera Vibrio, Photobacterium, Aeromonas, and Plesiomonas, together with the family Enterobacteriaceae, the family Pasteurellaceae, and probably the genus Alteromonas, form a robust monophyletic unit within the gamma 3 subgroup of the Proteobacteria.
URL: 7520733
Ref #: 27372
Author(s): Dorsch,M.;Lane,D.;Stackebrandt,E.
Journal: Int J Syst Bacteriol
Title: Towards a phylogeny of the genus Vibrio based on 16S rRNA sequences
Volume: 42
Page(s): 58-63
Year: 1992
Keyword(s): GENBANK/L05178 GENBANK/X54744 GENBANK/X54745 GENBANK/X56575 GENBANK/X56576 GENBANK/X56577 GENBANK/X56578 GENBANK/X56579 GENBANK/X56580 GENBANK/X56581 GENBANK/X56582 GENBANK/X56583 Base Sequence Molecular Sequence Data Phylogeny RNA Probes RNA, Bacterial/*chemistry RNA, Ribosomal, 16S/*chemistry Vibrio/*classification/genetics/isolation & purification
Remarks: The inter- and intrageneric relationships of the genus Vibrio were investigated by performing a comparative analysis of the 16S rRNAs of 10 species, including four pathogenic representatives. The results of immunological and 5S rRNA studies were confirmed in that the genus is a neighboring taxon of the family Enterobacteriaceae. With regard to the intrageneric structure, Vibrio alginolyticus, Vibrio campbellii, Vibrio natriegens, Vibrio harveyi, Vibrio proteolyticus, Vibrio parahaemolyticus, and Vibrio vulnificus form the core of the genus, while Vibrio (Listonella) anguillarum, Vibrio diazotrophicus, and Vibrio hollisae are placed on the outskirts of the genus. Variable regions around positions 80, 180, and 450 could be used as target sites for genus- and species-specific oligonucleotide probes and polymerase chain reaction primers to be used in molecular identification.
URL: 1371064
Ref #: 95445
Author(s): Eiler,A.;Bertilsson,S.
Journal: J Microbiol Methods
Title: Detection and quantification of Vibrio populations using denaturant gradient gel electrophoresis
Volume: 67
Page(s): 339-48
Year: 2006
Keyword(s): DNA, Bacterial/chemistry/genetics Electrophoresis, Polyacrylamide Gel/*methods Formamides/chemistry Oceans and Seas Organic Chemicals/chemistry Phylogeny Polymerase Chain Reaction RNA, Ribosomal, 16S/chemistry/genetics Sequence Analysis, DNA Sweden Urea/chemistry Vibrio/genetics/*isolation & purification *Water Microbiology
Remarks: Bacteria affiliated with the genus Vibrio are endemic in marine and estuarine ecosystems and are also found in many freshwater environments. Vibrios can enter viable but non-culturable states and since many species are pathogenic, there is a great need for culture-independent methods that identify and quantify multiple Vibrio populations. We adopted Vibrio-specific 16S rRNA-directed primers and a competitive PCR protocol (QC-PCR; [Thompson, J.R., Randa, M.A., Marcelino, L.A., Tomita-Mitchell, A., Lim, E., Polz, M.F., 2004b. Diversity and dynamics of a North Atlantic coastal Vibrio community. Appl. Environ. Microbiol. 70, 4103-4110]) for separation and quantification of Vibrio populations using denaturant gradient gel electrophoresis (DGGE). Sixteen Vibrio isolates and eight environmental samples were used to assess the precision and resolution of the method. A 45-70% gradient of Urea and formamide enabled separation of Vibrio populations with single nucleotide differences in the amplified fragment. A titration curve for the QC-PCR-DGGE, verified by amending surface water bacterioplankton samples with up to 3 x 10(5)Vibrio cholerae cells, could be approximated by a linear regression of log-transformed values (R(2)=0.96). The limit of detection for single populations was 180 cells per extracted sample or about 4 cells per PCR reaction. Environmental samples from the southern Stockholm archipelago in the Baltic Sea and the more saline coastal waters of Skagerrak each carried between 2 and 6 Vibrio populations, and there were major differences between the locations. Notably, multiple Vibrio populations could be detected and quantified against a background of native bacterioplankton exceeding Vibrio population abundance by more than 6 orders of magnitude. Putative identification based on migration in the DGGE gel was verified by parallel cloning and sequencing of PCR products, and representative clones were also characterized by DGGE. This general approach could also be useful for targeting other phylogenetically constrained bacterial groups and assess their abundance and distribution in complex environmental settings.
URL: 16730823
Ref #: 12044
Author(s): Ruimy,R.;Breittmayer,V.;Elbaze,P.;Lafay,B.;Boussemart,O.;Gauthier,M.;Christen,R.
Journal: Int J Syst Bacteriol
Title: Phylogenetic analysis and assessment of the genera Vibrio, Photobacterium, Aeromonas, and Plesiomonas deduced from small-subunit rRNA sequences
Volume: 44
Page(s): 416-26
Year: 1994
Keyword(s): GENBANK/X74674 GENBANK/X74675 GENBANK/X74676 GENBANK/X74677 GENBANK/X74678 GENBANK/X74679 GENBANK/X74680 GENBANK/X74681 GENBANK/X74682 GENBANK/X74683 GENBANK/X74684 GENBANK/X74685 GENBANK/X74686 GENBANK/X74687 GENBANK/X74688 GENBANK/X74689 GENBANK/X74690 GENBANK/X74691 GENBANK/X74692 GENBANK/X74693 GENBANK/X74694 GENBANK/X74695 GENBANK/X74696 GENBANK/X74697 GENBANK/X74698 GENBANK/X74699 GENBANK/X74700 GENBANK/X74701 GENBANK/X74702 GENBANK/X74703 Aeromonas/classification/genetics Comparative Study Photobacterium/classification/genetics *Phylogeny Plesiomonas/classification/genetics RNA, Bacterial/*genetics RNA, Ribosomal/*genetics Sequence Homology, Nucleic Acid Species Specificity Support, Non-U.S. Gov't Vibrio/classification/genetics Vibrionaceae/*classification/*genetics
Remarks: We sequenced nearly complete small-subunit rRNAs of 54 reference strains belonging to the genera Vibrio, Photobacterium, Aeromonas, and Plesiomonas. We then performed a phylogenetic analysis by comparing the sequences which we obtained with all other known sequences for bacteria belonging to the gamma subgroup of the Proteobacteria (thus providing a data base consisting of 70 sequences for the genera investigated), using methods such as neighbor joining, maximum likelihood, and maximum parsimony, as well as bootstrap, to assess the robustness of each topology. Our results confirmed that the family Vibrionaceae should include only Photobacterium and Vibrio species (but not Vibrio marinus); that Aeromonas species deserve family rank; and that Plesiomonas shigelloides is linked to the family Enterobacteriaceae. The genera Vibrio, Photobacterium, Aeromonas, and Plesiomonas, together with the family Enterobacteriaceae, the family Pasteurellaceae, and probably the genus Alteromonas, form a robust monophyletic unit within the gamma 3 subgroup of the Proteobacteria.
URL: 94347604
Ref #: 13615
Author(s): Dorsch,M.;Lane,D.;Stackebrandt,E.
Journal: Int J Syst Bacteriol
Title: Towards a phylogeny of the genus Vibrio based on 16S rRNA sequences
Volume: 42
Page(s): 58-63
Year: 1992
Keyword(s): GENBANK/L05178 GENBANK/X54744 GENBANK/X54745 GENBANK/X56575 GENBANK/X56576 GENBANK/X56577 GENBANK/X56578 GENBANK/X56579 GENBANK/X56580 GENBANK/X56581 GENBANK/X56582 GENBANK/X56583 Base Sequence Molecular Sequence Data Phylogeny RNA Probes RNA, Bacterial/*chemistry RNA, Ribosomal, 16S/*chemistry Support, Non-U.S. Gov't Vibrio/*classification/genetics/isolation & purification
Remarks: The inter- and intrageneric relationships of the genus Vibrio were investigated by performing a comparative analysis of the 16S rRNAs of 10 species, including four pathogenic representatives. The results of immunological and 5S rRNA studies were confirmed in that the genus is a neighboring taxon of the family Enterobacteriaceae. With regard to the intrageneric structure, Vibrio alginolyticus, Vibrio campbellii, Vibrio natriegens, Vibrio harveyi, Vibrio proteolyticus, Vibrio parahaemolyticus, and Vibrio vulnificus form the core of the genus, while Vibrio (Listonella) anguillarum, Vibrio diazotrophicus, and Vibrio hollisae are placed on the outskirts of the genus. Variable regions around positions 80, 180, and 450 could be used as target sites for genus- and species-specific oligonucleotide probes and polymerase chain reaction primers to be used in molecular identification.
URL: 92144377
Data: (ATCC 33564) Type strain / ATCC, USA / Anne Arundel General Hospital, Annapolis, USA / Stool / Hickman, F. W. et al. (1982) J. clin. Micro. 15, 395 / Validation List No. 9 (1982) Int. J. syst. Bact. 15, 384
Accession Date: 01/01/0
History: ARUNDEL A GENERAL HOSP ANNAPOLIS - ATCC
Authority: Hickman et al. 1982 (ALI)
Depositor: ATCC
Taxonomy: TaxLink: S3551 (Vibrio hollisae Hickman et al. 1982) - Date of change: 5/02/2003
Biosafety Responsibility: It is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country

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The Culture Collections hold cell cultures, bacteria, fungi and virus strains from worldwide sources. Our scientists ensure that the identification of the cultures is correct and they remain unchanged from when they are first deposited with the Collection. Nevertheless, some of the data we provide about the cultures is supplied by the person depositing the strains and, although we have multiple checking procedures in place, we cannot always verify all their data. Please note that the Culture Collections cannot be held responsible for any inaccuracies in the data provided by the depositors.

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