Extended Bibliography: |
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Ref #: |
31741 |
Author(s): |
Misawa,N.;Kawashima,K.;Kondo,F.;Kushima,E.;Kushima,K.;Vandamme,P. |
Journal: |
Vet Microbiol |
Title: |
Isolation and characterization of Campylobacter, Helicobacter, and Anaerobiospirillum strains from a puppy with bloody diarrhea |
Volume: |
87 |
Page(s): |
353-64 |
Year: |
2002 |
Keyword(s): |
GENBANK/AF497805
GENBANK/AF497806
GENBANK/AF497807
GENBANK/AF497808
GENBANK/AF497809
GENBANK/AF497810
GENBANK/AF497811
Animals
Bacterial Proteins/metabolism
Base Sequence
DNA, Bacterial/chemistry/genetics
Diarrhea/microbiology/*veterinary
Dog Diseases/drug therapy/*microbiology
Dogs
Electrophoresis, Polyacrylamide Gel/veterinary
Feces/microbiology
Female
Gram-Negative Bacteria/genetics/*isolation & purification/ultrastructure
Gram-Positive Bacterial Infections/drug therapy/microbiology/*veterinary
Microscopy, Electron/veterinary
Molecular Sequence Data
Polymerase Chain Reaction/veterinary
RNA, Ribosomal, 16S/chemistry/genetics
|
Remarks: |
We carried out a microscopic examination of stools from a 2-month-old female puppy with bloody diarrhea, and this revealed large numbers of different spiral-shaped bacteria. To isolate these organisms, a rectal swab specimen was inoculated onto plates of Skirrow's agar and incubated at 37 degrees C for 6 days in a microaerobic atmosphere. Finally, a total of six different spiral-shaped bacteria (strains G1104, 94105, FR106, B0101, 3J102, and J2103) were isolated. Based on their morphology, biochemical traits, whole-cell protein profiles, and analysis of their 16S rDNA sequences, they were identified as Campylobacter upsaliensis, Helicobacter cinaedi, 'Flexispira rappini', two Anaerobiospirillum spp. with different morphologies, and Helicobacter sp., respectively. Analysis of 16S rRNA gene sequence data for strains 94150 (H. cinaedi) and FR106 (F. rappini) revealed that this approach has limitations when identifying isolates to the species level because of a high degree of sequence homology between these species (>99%) and considerable sequence variation among different isolates within these species. The dog was treated orally with amoxicillin for 3 days, which resolved the diarrhea. However, 1 day after the last dose the bloody diarrhea recurred but regarded to six more days amoxicillin treatment. This suggests a bacterial cause for the diarrhea. The approach to identification to microaerobic spiral-shaped bacteria in diarrheic dogs can be applied further to characterize their role in diarrhea illness. |
URL: |
12069772 |
|
Ref #: |
95450 |
Author(s): |
Korczak,B.M.;Stieber,R.;Emler,S.;Burnens,A.P.;Frey,J.;Kuhnert,P. |
Journal: |
Int J Syst Evol Microbiol |
Title: |
Genetic relatedness within the genus Campylobacter inferred from rpoB sequences |
Volume: |
56 |
Page(s): |
937-45 |
Year: |
2006 |
Keyword(s): |
Bacterial Proteins/*genetics
Base Sequence
Campylobacter/*classification/*genetics
DNA Primers
DNA, Bacterial/chemistry/genetics
DNA, Ribosomal/chemistry/genetics
DNA-Directed RNA Polymerases/*genetics
Molecular Sequence Data
*Phylogeny
Polymerase Chain Reaction
RNA, Ribosomal, 16S/genetics
Sequence Analysis, DNA
Statistics
|
Remarks: |
The genus Campylobacter comprises 17 species, some of which are important animal and human pathogens. To gain more insight into the genetic relatedness of this genus and to improve the molecular tools available for diagnosis, a universal sequencing approach was established for the gene encoding the beta-subunit of RNA polymerase (rpoB) for the genus Campylobacter. A total of 59 strains, including the type strains of currently recognized species as well as field isolates, were investigated in the study. A primer set specific for Campylobacter species enabled straightforward amplification and sequencing of a 530 bp fragment of the rpoB gene. The 16S rRNA gene sequences of all of the strains were determined in parallel. A good congruence was obtained between 16S rRNA and rpoB gene sequence-based trees within the genus Campylobacter. The branching of the rpoB tree was similar to that of the 16S rRNA gene tree, even though a few discrepancies were observed for certain species. The resolution of the rpoB gene within the genus Campylobacter was generally much higher than that of the 16S rRNA gene sequence, resulting in a clear separation of most species and even some subspecies. The universally applicable amplification and sequencing approach for partial rpoB gene sequence determination provides a powerful tool for DNA sequence-based discrimination of Campylobacter species. |
URL: |
16627635 |
|
Ref #: |
12371 |
Author(s): |
Misawa,N.;Kawashima,K.;Kondo,F.;Kushima,E.;Kushima,K.;Vandamme,P. |
Journal: |
Vet Microbiol |
Title: |
Isolation and characterization of Campylobacter, Helicobacter, and Anaerobiospirillum strains from a puppy with bloody diarrhea |
Volume: |
87 |
Page(s): |
353-64 |
Year: |
2002 |
Keyword(s): |
GENBANK/AF497805
GENBANK/AF497806
GENBANK/AF497807
GENBANK/AF497808
GENBANK/AF497809
GENBANK/AF497810
GENBANK/AF497811
Animal
Bacterial Proteins/metabolism
Base Sequence
Case Report
DNA, Bacterial/chemistry/genetics
Diarrhea/microbiology/*veterinary
Dog Diseases/drug therapy/*microbiology
Dogs
Electrophoresis, Polyacrylamide Gel/veterinary
Feces/microbiology
Female
Gram-Negative Bacteria/genetics/*isolation & purification/ultrastructure
Gram-Positive Bacterial Infections/drug therapy/microbiology/*veterinary
Microscopy, Electron/veterinary
Molecular Sequence Data
Polymerase Chain Reaction/veterinary
RNA, Ribosomal, 16S/chemistry/genetics
|
Remarks: |
We carried out a microscopic examination of stools from a 2-month-old female puppy with bloody diarrhea, and this revealed large numbers of different spiral-shaped bacteria. To isolate these organisms, a rectal swab specimen was inoculated onto plates of Skirrow's agar and incubated at 37 degrees C for 6 days in a microaerobic atmosphere. Finally, a total of six different spiral-shaped bacteria (strains G1104, 94105, FR106, B0101, 3J102, and J2103) were isolated. Based on their morphology, biochemical traits, whole-cell protein profiles, and analysis of their 16S rDNA sequences, they were identified as Campylobacter upsaliensis, Helicobacter cinaedi, 'Flexispira rappini', two Anaerobiospirillum spp. with different morphologies, and Helicobacter sp., respectively. Analysis of 16S rRNA gene sequence data for strains 94150 (H. cinaedi) and FR106 (F. rappini) revealed that this approach has limitations when identifying isolates to the species level because of a high degree of sequence homology between these species (>99%) and considerable sequence variation among different isolates within these species. The dog was treated orally with amoxicillin for 3 days, which resolved the diarrhea. However, 1 day after the last dose the bloody diarrhea recurred but regarded to six more days amoxicillin treatment. This suggests a bacterial cause for the diarrhea. The approach to identification to microaerobic spiral-shaped bacteria in diarrheic dogs can be applied further to characterize their role in diarrhea illness. |
URL: |
22066012 |
|
Ref #: |
3940 |
Journal: |
Int. J. Syst. Bacteriol. |
Title: |
Validation of the publication of new names and new combinations previously effectively published outside the IJSB. List No. 37. |
Volume: |
41 |
Page(s): |
331 |
Year: |
1991 |
|
Ref #: |
4220 |
Author(s): |
Vandamme,P.;Falsen,E.;Rossau,R.;Hoste,B.;Segers,P.;Tytgat,R.;DeLey,J. |
Journal: |
Int. J. Syst. Bacteriol. |
Title: |
Revision of Campylobacter, Helicobacter, and Wolinella taxonomy: emendation of generic descriptions and proposal of Arcobacter gen. nov. |
Volume: |
41 |
Page(s): |
88-103 |
Year: |
1991 |
|
Ref #: |
4332 |
Author(s): |
Moss,C.W.;Lambert-Fair,M.A.;Nicholson,M.A.;Guerrant,G. |
Journal: |
J. Clin. Microbiol. |
Title: |
Isoprenoid quinones of Campylobacter cryaerophila, C. cinaedi, C. fennelliae, C. hyointestinalis, C. pylori, and "C. upsaliensis". |
Volume: |
28 |
Page(s): |
395-397 |
Year: |
1990 |
|
Ref #: |
4739 |
Author(s): |
Goossens,H.;Pot,B.;Vlaes,L.;vandenBorre,C.;vandenAbbeele,R.;vanNaelten,C.;Levy,J.;Cogniau,H.;Marbehant,P.;Verhoef,J.;Kersters |
Journal: |
J. Clin. Microbiol. |
Title: |
Characterization and description of "Campylobacter upsaliensis" isolated from human feces. |
Volume: |
28 |
Page(s): |
1039-1046 |
Year: |
1990 |
|
Ref #: |
5020 |
Author(s): |
Sandstedt,K.;Ursing,J. |
Journal: |
System. Appl. Microbiol. |
Title: |
Description of Campylobacter upsaliensis sp. nov. previously known as the CNW group. |
Volume: |
14 |
Page(s): |
39-45 |
Year: |
1991 |
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