Bacteria and Mycoplasmas detail

Conditions of Supply of Microbial Pathogens: Safety

Bacteria Collection: Legionella wadsworthii

NCTC Number:

NCTC 11532

Current Name:

Legionella wadsworthii

Original Strain Reference:

Wadsworth 81-716A

Other Collection No:

ATCC 33877; WADSWORTH 81-716A

Previous Catalogue Name:

Legionella wadsworthii

Type Strain:

Yes

Family:

Legionellaceae

Hazard Group (ACDP):

Release Restrictions:

Terms & Conditions of Supply of Microbial Pathogens: Safety

Conditions for growth on solid media:

Buffered Charcoal Yeast Extract agar (BCYE), 48 hours, 37°C, requires carbon dioxide

Isolated From:

human, sputum

Whole Genome Sequence:

http://www.ebi.ac.uk/ena/data/view/ERS956173

Annotated Genome:

ftp://ftp.sanger.ac.uk/pub/project/pathogens/NCTC3000/...

16S rRNA Gene Sequence:

>gb|X73401|ATCC 33877|L.wadsworthii (ATCC 33877) gene for 16S rRNA.| gattgaacgctggcg...

23S rRNA Gene Sequence:

>gb|Z30462|81-716A|ATCC 33877|L.wadsworthii 81-716A genes for 23S ribosomal RNA and ribosomalRNA.| gcctccctcaagatg... >gb|Z24720|81-716A|ATCC 33877|L.wadsworthii 23S and 5S rRNA genes, partial CDSs.| gcctccctcaagatg... >gb|Z24720|81-716A|ATCC 33877|L.wadsworthii 23S and 5S rRNA genes, partial CDS's.| gcctccctcaagatg...

Bibliography:

EDELSTEIN P H ET AL 1982 ANN INT MED 97 809 812

Extended Bibliography:

Show bibliography

Ref #:	54192
Author(s):	Ko,K.S.;Lee,H.K.;Park,M.Y.;Lee,K.H.;Yun,Y.J.;Woo,S.Y.;Miyamoto,H.;Kook,Y.H.
Journal:	J Clin Microbiol
Title:	Application of RNA polymerase beta-subunit gene (rpoB) sequences for the molecular differentiation of Legionella species
Volume:	40
Page(s):	2653-8
Year:	2002
Keyword(s):	Bacterial Proteins/genetics Bacterial Typing Techniques Base Sequence DNA, Bacterial/genetics DNA-Directed RNA Polymerases/genetics Genes, Bacterial Humans Immunophilins/genetics Legionella/classification/enzymology/genetics/isolation & purification Membrane Proteins/genetics *Peptidylprolyl Isomerase Phylogeny RNA, Bacterial/genetics RNA, Ribosomal, 16S/genetics Serotyping Species Specificity
Remarks:	The nucleotide sequences of the partial rpoB gene were determined from 38 Legionella species, including 15 serogroups of Legionella pneumophila. These sequences were then used to infer the phylogenetic relationships among the Legionella species in order to establish a molecular differentiation method appropriate for them. The sequences (300 bp) and the phylogenetic tree of rpoB were compared to those from analyses using 16S rRNA gene and mip sequences. The trees inferred from these three gene sequences revealed significant differences. This sequence incongruence between the rpoB tree and the other trees might have originated from the high frequency of synonymous base substitutions and/or from horizontal gene transfer among the Legionella species. The nucleotide variation of rpoB enabled more evident differentiation among the Legionella species than was achievable by the 16S rRNA gene and even by mip in some cases. Two subspecies of L. pneumophila (L. pneumophila subsp. pneumophila and subsp. fraseri) were clearly distinguished by rpoB but not by 16S rRNA gene and mip analysis. One hundred and five strains isolated from patient tissues and environments in Korea and Japan could be identified by comparison of rpoB sequence similarity and phylogenetic trees. These results suggest that the partial sequences of rpoB determined in this study might be applicable to the molecular differentiation of Legionella species.
URL:	12089300

Ref #:	95439
Author(s):	Robinson,P.N.;Heidrich,B.;Tiecke,F.;Fehrenbach,F.J.;Rolfs,A.
Journal:	FEMS Microbiol Lett
Title:	Species-specific detection of Legionella using polymerase chain reaction and reverse dot-blotting
Volume:	140
Page(s):	111-9
Year:	1996
Keyword(s):	GENBANK/Z30431 GENBANK/Z30432 GENBANK/Z30433 GENBANK/Z30434 GENBANK/Z30435 GENBANK/Z30436 GENBANK/Z30437 GENBANK/Z30438 GENBANK/Z30439 GENBANK/Z30440 GENBANK/Z30441 GENBANK/Z30442 GENBANK/Z30443 GENBANK/Z30444 GENBANK/Z30445 GENBANK/Z30446 GENBANK/Z30447 GENBANK/Z30448 GENBANK/Z30449 GENBANK/Z30450 GENBANK/Z30451 GENBANK/Z30452 GENBANK/Z30453 GENBANK/Z30454 GENBANK/Z30455 GENBANK/Z30534 GENBANK/Z30535 GENBANK/Z30536 GENBANK/Z30537 GENBANK/Z30538 etc. Base Sequence DNA Primers/genetics DNA Probes/genetics DNA, Bacterial/genetics/isolation & purification DNA, Ribosomal/genetics Humans Legionella/classification/genetics/isolation & purification Legionnaires' Disease/diagnosis/microbiology Molecular Sequence Data Polymerase Chain Reaction/*methods RNA, Bacterial/genetics RNA, Ribosomal, 23S/genetics RNA, Ribosomal, 5S/genetics Sequence Homology, Nucleic Acid Species Specificity
Remarks:	Legionella pneumophila and some other Legionella species are capable of causing Legionnaire's disease, a potentially fatal pneumonia. The identification of legionellae by standard laboratory techniques such as culture is difficult and time consuming. In the present work, the DNA sequence of the 23S-5S spacer region was determined for 43 Legionella isolates, and the sequence information was used to develop a species-specific detection system using PCR and reverse dot-blotting which employs just one PCR amplicon to perform genus- and species-specific detection. L. pneumophila serogroups 1-16 as well as 21 non-pneumophila isolates could be identified and differentiated at the species level using this system.
URL:	8764471

Ref #:	13685
Author(s):	Robinson,P.N.;Heidrich,B.;Tiecke,F.;Fehrenbach,F.J.;Rolfs,A.
Journal:	FEMS Microbiol Lett
Title:	Species-specific detection of Legionella using polymerase chain reaction
Volume:	140
Page(s):	111-119
Year:	1996
Keyword(s):	0 (DNA Primers) 0 (DNA Probes) 0 (DNA, Bacterial) 0 (DNA, Ribosomal) 0 (RNA, Bacterial) 0 (RNA, Ribosomal, 23S) 0 (RNA, Ribosomal, 5S) Base Sequence Comparative Study DNA Primers/genetics DNA Probes/genetics DNA, Bacterial/genetics/isolation & purification DNA, Ribosomal/genetics Human Legionella/classification/genetics/isolation & purification Legionnaires' Disease/diagnosis/microbiology Molecular Sequence Data Polymerase Chain Reaction/*methods RNA, Bacterial/genetics RNA, Ribosomal, 23S/genetics RNA, Ribosomal, 5S/genetics Sequence Homology, Nucleic Acid Species Specificity
Remarks:	Legionella pneumophila and some other Legionella species are capable of
URL:	96291654

Data:

(ATCC 33877) Type strain / ATCC in 1983 / P. H. Edelstein / Sputum in 1981 / Edelstein, P.H. et al. (1982) Ann. Int. Med. 97, 809

Accession Date:

01/01/1983

Authority:

Edelstein et al. 1983

Depositor:

PIENTA P, ATCC

Taxonomy:

TaxLink: S1652 (Legionella wadsworthii Edelstein et al. 1983) - Date of change: 5/02/2003

Biosafety Responsibility:

It is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country

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Note:

The Culture Collections hold cell cultures, bacteria, fungi and virus strains from worldwide sources. Our scientists ensure that the identification of the cultures is correct and they remain unchanged from when they are first deposited with the Collection. Nevertheless, some of the data we provide about the cultures is supplied by the person depositing the strains and, although we have multiple checking procedures in place, we cannot always verify all their data. Please note that the Culture Collections cannot be held responsible for any inaccuracies in the data provided by the depositors.

Cultures supplied by Culture Collections are to be used as controls for microbiology testing and for research purposes only. Please view the Terms & Conditions of Supply for more information.